In Vitro DIGESTIBILITY EVALUATION OF RICE BRAN MEAL INCORPORATED WITH PRECIPITATE p-D MANNANASE FROM Penicillium italicum

The effect of addition of crude and ammonium sulphate ((NH^SQi) precipitation of P-D-mannanase produced by Penicillium italicum on in vitro dry matter digestibility (IVDMD) and in vitro protein digestibility (IVPD) of rice bran meal intended for animal feed formulation was studied. Firstly, the crude enzyme (non-precipitated), and (NH-1)SO4 precipitated enzyme were incorporated in the series of enzymes activities. Secondly, the effect of p-D-mannanase preparation and incubation time was evaluated on IVDMD and IVPD. Crude enzyme produced in basal medium supplemented with rice bran meal as the carbon source was assayed by dinitrosalicylic acid method and precipitated from culture supernatant with 70% ammonium sulphate concentration. Measurement of IVDMD and IVPD were carried out using pepsin pancreatine assay. Results from statistical analysis showed significant interaction between the kind of enzyme preparations and activities on IVPD and IVDMD. In vitro protein digestibility (IVPD) of rice bran meal prepared with different enzyme preparations increased significantly (P