Olu Odeyemi

A Chemically Defined Medium for Growth of Rhizobium

A new synthetic medium was developed for growth of rhizobia. The medium consisted of mineral salts amended with 0.5% mannitol and one or two growth factors. The vitamins calcium pantothenate, thiamine mononitrate, inositol, and biotin stimulated rhizobial growth. In particular, calcium pantothenate was found to be almost as effective as a combinati...