Research Article

Antibiotic Susceptibility and Phenotypic Plasmid Screening Among Escherichia coli Isolated from Abattoir Wastewater in Bauchi State, Nigeria

Hassan, K. S.1*, Bukar, A.2, Mohammed, B.3, Ishaq, S. A4, Abubakar, A. I.4, Yakubu, H.1
1 Department of Microbiology, Faculty of Sciences, Bauchi State University, Gadau
2 Department of Microbiology, Faculty of Life Sciences, College of Natural and Pharmaceutical Sciences, Bayero University, PMB 3011, Kano state, Nigeria
3 Department of Microbiology, Bayero University, Kano, Nigeria.
4 Department of Microbiology, Faculty of Sciences Federal University Gusau, Zamfara State Nigeria
* Corresponding author: khsambo@basug.edu.ng
Published: Jun, 2020
DOI: 10.67614/njm.2020.vs81as66
Pages: 5153-5161

Abstract

This study highlighted the antibiotic susceptibility and the emergence of multidrug resistance plasmids among Escherichia coli in abattoir wastewater in Bauchi state Nigeria. Isolation and characterization of E. coli was conducted from 150 samples using standard procedures. Antibiotic susceptibility testing and plasmid curing were done on the strains. Of these samples screened only 18 (12%) E. coli were recovered. Antibiotic susceptibility testing showed high resistance to Augmentin (77.7%) with a clear diameter of inhibition ranging between 8.0±0.0 – 17.3±1.4 mm, followed by amoxicillin (8.0±0.0 – 17.3±1.4) mm, streptomycin (8.0±00 – 10.0±2.6) mm and septrin (8.0±0.0 – 10.3±0.3) mm, with 61.1%, each, and gentamicin (8.0±0.0 – 17.3±0.0) mm and chloramphenicol (8.0±0.0 – 11.0±2.0) mm, each with 55.5% respectively. Ciprofloxacin (18.0±0.0 - 40.0±0.5) mm, was the most potent with 83.3% activity. Multiple antibiotic resistance was examined in 12 (66.6%) of the isolates. After curing, antibiotic susceptibility testing most of the isolates were observed to harbor plasmid-mediated resistance. This study has revealed the emergence of multidrug plasmids mediated resistance among Escherichia coli in abattoir wastewater in Bauchi State Nigeria.

Keywords

Abattoir Escherichia coli Plasmid Resistance Waste water

References

  1. Abiade-Paul, C., Kene, I. and Chah, K. (2005). Occurrence and Antibiogram of Salmonellae in Effluent from Nsukka Municipal Abattoir; Nigerian Veterinary Journal, 27(1): 48-53.
  2. Adeyemo, I.G. and Adeyemo, O.K. (2007). Waste Management Practices at the Bodija Abattoir, Nigeria. International Journal of Environmental Studies, 64(1): 71–82.
  3. Adeyemo, O. (2002). Unhygienic Operation of a City Abattoir in South Western Nigeria: Environmental Implication.
  4. African Journal of Environmental Assessment and Management, 4(1): 23-28.
  5. Ash, R.J.; Mauck, B.; Morgan, M. (2002) Antibiotic Resistance of Gram-Negative Bacteria in Rivers, United States. Emergence Infectious Disease: 8:713-716.
  6. Atieno, N. R., and Owuor, O. P. (2012). Isolation of High Antibiotic Resistant Fecal Bacteria Indicators, Salmonella and Vibrio Species from Raw Abattoirs Sewage in Peri-Urban Locations of by. http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=265781&tool=pmcentrez&rendertype=abstract.
  7. Benka-Coker, M. and Ojior, O., 1995. Effect of Slaughterhouse Wastes on the Water Quality of Ikpoba River, Nigeria. Bioresource Technology, 52, pp.5–12.
  8. Centers for Disease Control and Prevention (CDC), 2014 United State's National Action Plan for Combating Antibiotic-Resistant Bacteria. Executive summary.
  9. Cheesbrough M (2000). District Laboratory Practice in Tropical Countries (Part 11). Cambridge, University Press UK. pp. 134-143
  10. Chukwu, O., 2008. Analysis of Groundwater Pollution from Abattoir Waste in Minna, Nigeria. Research Journal of Dairy Sciences: 2(4) Pp:74–77
  11. Cowan, S. T. and Steel, K. J. (1993). "Manual for Identification of Medical Bacteria". Cambridge University Press.3rd Edition. Pp. 199 - 241
  12. CLSI (2008). Clinical and Laboratory Standards Institute. Performance Standards for Antimicrobial Susceptibility Testing. Eighteenth Informational Supplement. M100-S18 vol. 28 no.1
  13. Graf, Fabrice E, Martin Palm, Marie Abadikhah, and Martin Zackrisson. 2018. "Escherichia Coli Causing Urinary Tract Infections: A Case Study From Nigeria." British Journal of Applied Science & Technology.204-210 4(1)
  14. Hadley, H. R. (2002). Vesicovaginal fistula. Current Urology, 3(5):301-7.
  15. Hughes, C.; Bauer, E., Roberts, A. P. (1981) Spread of r-plasmids among E. coli Causing Urinary Tract Infection. Antimicrobial Agents' chemotherapy: 20, 496-502.
  16. Idika, N. (1999) the Efficacy of Water Purification Methods in the Control of Diarrhoeal Pathogens in Rural Communities of Lagos State; Ph.D. Thesis; University of Lagos; Nigeria, 53.
  17. Igwe, J. C. Omaolapo, J. A, Dauda, E O and Olaidipo, H. O (2013) Plasmid Conjugation in E. coli and Drug Resistance. Nigerian Journal of Biotechnology Vol. 26 (2013) 41 – 45.
  18. Jorge, Gabriela, Da Silva, and Sara Domingues. 2016. "Insights on the Horizontal Gene Transfer of Carbapenemase Determinants in the Opportunistic Pathogen Acinetobacter Baumannii." 1–23. https://doi.org/10.3390/microorganisms4030029.
  19. Marquez, C., Lasbate, M., Raymondo, C., Fernandez, J., Gestol, A. M., Holley, M., Borthgaray, G., Stokes, H. W. (2008). Urinary Tract Infections in a South American Population: Dynamic Spread of Class 1 Integrons and Multidrug Resistance by Homologous and Site-specific Recombination. Journal of Clinical Microbiology: 46, 3417-3425.
  20. Nwanta, J.A., Onunkwo, J. and Ezenduka, E., 2010. Analysis of Nsukka Metropolitan Abattoir Solid Waste and Its Bacterial Contents in South Eastern Nigeria: Public Health Implication. Archives of environmental & occupational health, 65(1), pp.21–6.
  21. Oluwole Adebayo Daini, Helen Opeoluwa Adegboyega, Kubura Temitope Odufuwa and David Olusoga Ogbolu. (2011) Incidence of Multidrug Resistance R-Plasmids Among Escherichia Coli Causing Urinary Tract Infections: A Case Study From Nigeria. British Journal of Applied Science & Technology 204-210 4(1)
  22. Pignato, S, M A Coniglio, G Faro, F X Weill, and G Giammanco. (2009). "Plasmid-Mediated Multiple Antibiotic Resistance of Escherichia Coli in Crude and Treated Wastewater Used in Agriculture," 251–58. https://doi.org/10.1016/wh.2009.019.
  23. Prescott, M.L., Harley, J.P. and Klein, D.A. (1999).Microbiology.4th Edn. The Tata McGraw Hill, New York. 792-802.
  24. Rasool, S A; Ashmad, S, Skhan And A. Wahab,(2003) Plasmi Borne Antibiotics Resistance Factors Among Indigenous Kleibsehilla. Pakistan Journal of Botany: 35:243-248
  25. Sherley, M., Gordon, D.M and Collignon, P.J. (2004). Evolution of Multi-Resistance Plasmids in Australian Clinical Isolates of Escherichia Coli. Microbiology 150:1539-1546.
  26. Ojo O. S. K. S, B. O. Sargin and F.I. Esunwh (2014)Plasmid Curing Analysis of Antibiotic Resistance in B-Lactamase Producing Stahylococci
  27. Svanström, p. (2014). Pathogens and Antibiotic Resistant Bacteria in Abattoir Waste and Animals – A Study Involving Abattoir Wastewater, Earthworms and Marabou Storks. Swedish University of Agricultural Sciences.
  28. Taylor, L.H., Latham, S.M. and Woolhouse, M.E., (2001). Risk Factors for Human Disease Emergence. Philosophical Transactions of the Royal Society of London. Series B, Biological sciences: 356(1411), pp.983–9.
  29. Verheijen, L.A.H.M., Wiersema, D. and Hulshoff Pol, L.W. (1996). Management of Waste from Animal Product Processing. Available at: http://www.fao.org/WAIRDOCS/LEAD/X6114E/X6114E00.HTM (2013-08-22)
  30. Yah S.C, N. O Eghafona, S. Oranusi and A. M Abouo (2014). Widespread Plasmid Resistance Genes among Proteus Species in Diabetic Wounds of Patients in the Ahmadu Bello University Teaching Hospital (ABUTH) Zaria. African Journal of Biotechnology: 6:1757-1762.
How to Cite

S., H. K., A., B., B., M., A, I. S., I., A. A., & H., Y. (2020). Antibiotic Susceptibility and Phenotypic Plasmid Screening Among Escherichia coli Isolated from Abattoir Wastewater in Bauchi State, Nigeria. Nigerian Journal of Microbiology, 34(1), 5153-5161. https://doi.org/10.67614/njm.2020.vs81as66

H. K. S., B. A., M. B., I. S. A, A. A. I., and Y. H., "Antibiotic Susceptibility and Phenotypic Plasmid Screening Among Escherichia coli Isolated from Abattoir Wastewater in Bauchi State, Nigeria," Nigerian Journal of Microbiology, vol. 34, no. 1, pp. 5153-5161, June 2020. doi: 10.67614/njm.2020.vs81as66

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