Research Article

Antibiotic Susceptibility Profile of Microbial Isolates From Open Wounds To Common Antibiotics

Adigun, M.O.1*, Afolabi, O.O.1, Agbaje-Daniels, F.V1, Okonofua, C.C.1, Lateef, S.A.1
1 Biological Sciences Department, Crawford University, Faith City, Igbesa, Ogun State. Nigeria
* Corresponding author: micadigun@crawforduniversity.edu.ng
Published: Jun, 2018
Pages: 4148-4157

Abstract

Wound healing is a complex and dynamic process with the wound environment changing with the changing health status of the individual. This is a resultant effect of antibiotic resistance which the World Health Organization (WHO) has declared as pandemic since 2012. To consolidate this fact, forty-one (41) open wound swab samples were collected from Lagos State University Teaching Hospital (LASUTH)and analyzed microbiologically to identify to species level. The use of the microbact 12a/12b and 24 eidentification kits was employed. Fifty-four (54) bacterial isolates were isolated comprising of ten different bacteria organisms with their percentage prevalence, videlicet; Staphylococcus aureus (33.33%), Pseudomonas aeruginosa (20.37%), Klebsiella pneumoniae (14.81%), Escherichia coli(9.25%), Acinetobacta iwojfi (7.41%), Klebsiella oxytoca (5.56%), Proteus mirabilis(3.10%), Proteus vulgaris (1.85%), Acinetobacta baumani (1.85%) and Escherichia coli-inactive (1.85%). The isolates were cultured on MacConkey, mannitol salt, Eosin methylene blue and nutrient agar. Gram staining technique was used to determine the Gram- positive and Gram- negative bacteria, after culturing, sensitivity test was done on all the isolated bacteria with Mueller-Hilton agar using Kirby-bauer technique, and the following antibiotics ceftazidime (CAZ) 30ug, cefuroxime (CRX) 30ug, gentamicin (GEN) lOug, cefriaxone (CTR) 30ug, erytheomycin (ERY) 5ug, cloxacilin (CXC) 5ug, ofloxacin (OFL) 5ug, augmentin (AUG) 30u.g. Only ofloxaxin was found to be very effective followed by gentamicin, the rest of the antibiotics were ineffective against the microorganisms as they were multiple drug resistant

Keywords

antibiotics drug resistant microbes open wounds sensitivity test

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How to Cite

M.O., A., O.O., A., F.V, A., C.C., O., & S.A., L. (2018). Antibiotic Susceptibility Profile of Microbial Isolates From Open Wounds To Common Antibiotics. Nigerian Journal of Microbiology, 32(1), 4148-4157.

A. M.O., A. O.O., A. F.V, O. C.C., and L. S.A., "Antibiotic Susceptibility Profile of Microbial Isolates From Open Wounds To Common Antibiotics," Nigerian Journal of Microbiology, vol. 32, no. 1, pp. 4148-4157, June 2018.

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