Research Article

Antibiotics Resistance in Haemolytic Bacterial pathogens from Hospital Wastewaters

Obuekwe, I. S1*, Osariemen. P. O.1
1 Department of Microbiology, Faculty of Life Sciences, University of Benin, Benin City, Edo State, Nigeria
* Corresponding author: ifeyinwa.obuekwe@uniben.edu
Published: Jun, 2020
DOI: 10.67614/njm.2020.kt47ahjo
Pages: 4945-4953

Abstract

Burden of morbidities and mortalities originating from bacterial pathogens and antibiotics resistance is on the rise, and hospital wastewaters are possible reservoirs of these pathogens. Infectious diseases and antibiotic resistance from Hospital Wastewaters could be spread into the environment. This study aimed at isolation, identification and antibiotic susceptibility characterization of hemolytic bacterial pathogens from hospitals wastewaters in Benin City, Edo State, Nigeria. Hospital wastewaters (HWWs) were aseptically collected from four major Hospitals in Benin City, and selective media were used for bacterial isolation while identification was based on standard procedures. Sheep blood agar was used for hemolytic bacterial isolation, and isolates were subsequently assessed for Antibiotic resistance to common antibiotics using Kirby-Bauer disc diffusion method. Hemolytic bacterial pathogens recovered were Pseudomonas aeruginosa (23 %), Staphylococcus aureus (19.2 %), Salmonella spp (30.7 %), Escherichia coli (19.2 %) and Escherichia coli O157: H7 (7.6 %). High resistance against Augumentin (25 µg) was observed in Salmonella spp (90.6 %), Pseudomonas aeruginosa (79.2 %), Escherichia coli (50 %) and Escherichia coli O157: H7 (37.5 %). Conversely, Staphylococcus aureus (55 %) isolates were resistant to Amoxicillin (30 µg). All Gram negative hemolytic pathogens showed low resistance to Sparfloxacin (10 µg) and Ofloxacin (30 µg). Multiple antibiotics resistance index (MARI) greater than 0.2 was observed in 50 % of the hemolytic pathogens. Antibiotics resistance in hemolytic bacterial pathogens obtained in this study suggests their potential as sources of environmental and public health risks.

Keywords

Antibiotics Bacterial pathogens Hemolytic bacteria Hospital wastewaters

References

  1. Abakpa, G.O., Umoh, V.J., Ameh, J.B., Yakubu, S.E., Kwaga, J.K.P and Kamaruzaman, S. (2015). Diversity and antimicrobial resistance of Salmonella enterica isolated from fresh produce and environmental samples. Environmental Nanotechnology, Monitoring and Management 3: 38-46.
  2. Adekunle, O.A., Mary, A.B., Oyinade, A.A., Bridget, O.F. and Adebanke, O.A. (2011). Antibiotics sensitivity patterns of Escherichia coli and Aerobacter aerogenes isolated from waste water in Ile- Ife, Nigeria. International Journal of Medicine and Medical Sciences 3: 155-160.
  3. Adenaike, O., Olonitola, O.S., Ameh, J.B and Whong, C.M.Z. (2016). Multidrug Resistance and Multiple Antibiotics Resistance Index of Escherichia coli strains Isolated from Retailed smoked Fish. Journal of Natural Science Research 6: 7-10.
  4. Akubuenyi, F.C., G.E. Arikpo, G.E., Ogugbue, C.J., Mfongeh, J.F. and Akpanumun, E.V. (2011). Antibiotic resistance profile of Waterwater isolates obtained from University of Calabar Teaching Hospital and General Hospital Calabar, Nigeria. Nigerian Journal of Microbiology 25: 2243-2255.
  5. Amechi, S.N., Chima, V.N., Nwanneka, C.N. and Herbert, C.E. (2016). Evaluation and antimicrobial susceptibility pattern of pathogenic bacteria in hospital wastewater. International Journal of Microbiology and Biotechnology 1: 10-15.
  6. Amores, M.J, Meneses, M, Pasqualino, J, Antón, A. and Castells, F. (2013). Environmental assessment of urban water cycle on mediterranean conditions by LCA approach. Journal of Cleaner Production 43: 84-92.
  7. Bauer, A.W, Kirby, W. M, Sheris, J.C. and Turck, M. (1966). Antibiotic susceptibility by Standardized single disk method. American Journal of clinical Pathology 45: 493-496.
  8. Beyene, H. and Redaie, G. (2011). Assessment of Waste Stabilization Ponds for the Treatment of Hawassa University Referral Hospital. World Applied Sciences 15:142-150.
  9. Bolaji, A.S., Akande, I.O., Iromini, F.A., Adewoye, S.O. and Opasola, O.A. (2012). Antibiotic resistance pattern of bacteria spp isolated from the sewage of the University of Cocody, Abidjan, Côte d'Ivoire. International Journal of Tropical Disease and Health 4: 608-620.
  10. Carraro, E., Bonetta, S., Bertino, C., Lorenzi, E., Bonetta, S. and Gilli, G. (2016). Hospital effluents management: Chemical, physical, microbiological risks and legislation in different countries. Journal of Environmental Management 168:185-199.
  11. Cheesebrough, M. (2006). Disraica laboratory Practice in Tropical Countries Part 2, 2nd edition, Cambridge University Press. P. 70-78.
  12. Clinical Laboratory Standard Institute (CLSI) (2017). Performance Standards for Antimicrobial Susceptibility Testing, 27th edition. CLSI supplement M100S. Wayne, Pennsylvania P.250.
  13. Diab, A.M., Al-Turk, I.M, Ibrahim, M.K. and Al-delmarzhrany, D.K. (2008). Tracing of Gram-negative antibiotic-resistant bacteria in hospital wastewater at Al-Madinah Al-Mounwwarah. Journal of Taibah University for Science 1: 24-34.
  14. Divyakolu, K., Rosy Chikkala, R., Ratnakar, K.S, Sritharan, V. (2019). Hemolysins of Saphylococcus aureus - An Update on Their Biology, Role in Pathogenesis and as Targets for Anti-Virulence Therapy. Advances in Infectious Diseases 9: 80-104.
  15. Fair, R.J and Tor, Y. (2014). Antibiotics in the 21st Century. Perspectives in medicinal chemistry 6: 25-64.
  16. Imanah, E.O., Abeni, B. and Igbinosa, E.O. (2017). Antibiogram profile of Pseudomonas aeruginosa isolated from some selected hospital environmental drains. Asian Pacific Journal of Tropical Disease 7: 604-609.
  17. Julien, C.K., Gabazé, G.A.A., Kernard, K., Bernard, Y. O. and Mireille, D. (2014). The antibiotic resisting profile of Salmonella spp.
  18. Keen, P.L. and Patrick, D.M. (2013). Tracking the Ecological Footprint of Antibiotics and Antimicrobial Resistance. Antibiotics Review 2:191-205.
  19. Korzeniewska, E., Korzeniewska, A. and Harmisz, M. (2013). Antibiotic resistant Escherichia coli in hospital and municipal sewage and their emission to the environment. Ecotoxicology and Environmental Safety 91:96-102.
  20. Leclercq, S. Y., Sullivan, M.J., Ipe, D.S., Smith, J.P., Cripps, A.W. and Ulett, G.C. (2016). Pathogenesis of Streptococcus urinary tract infection depends on bacterial strain and β-hemolysin/cytolysin that mediates cytotoxicity, cytokine synthesis, inflammation and virulence. Scientific Reports 6: 29000 | DOI: 10.1038/srep29000.
  21. Mandal, M.D, Mandal, S. and Pal, N.K. (2011). Antibiotic resistance prevalence and pattern in environmental bacterial isolates. The Open Antimicrobial Agents Journal 3: 45-52.
  22. Moges, F., Mengistu, E., Yeshambel B. and Walelegn, W. (2014). Isolation and characterization of multiple drug resistance bacterial pathogens from wastewater in hospital and non-hospital environments, Northwest Ethiopia. BioMed Central (BMC) Research Notes 7:215-221.
  23. Nunez, L. and Moretton, J. (2007). Disinfectant-Resistant Bacteria in Buenos Aires City Hospital Wastewater. Brazilian Journal of Microbiology 38:644-648.
  24. Onuoha, S.C (2017). Isolation and Characterization of Multi-drug Resistant Bacteria spp isolated from hospital waste water in Ede South Western, Nigeria.
  25. Pavlov, D., De-Wet, C.M.E., Grabow, W. O.K and Ehlers, M.M. (2004). Potentially pathogenic features of heterotrophic plate count bacteria isolated from treated and untreated drinking water. International Journal of Food Microbiology 92: 275-287.
  26. Singh, B.R., Singh, V.P., Agarwal, M., Sharma, G., Chandra, M. (2004). Haemolysins of Salmonella, their role in pathogenesis and subtyping of Salmonella serovars. Indian Journal of Experimental Biology 42 (3):303-313.
  27. Stalder, T., Barraud, O., Jove, T., Casellas, M., Gaschet, M., Dagot, C. and Ploy, M.C. (2014). Quantitative impact of hospital effluent on qualitative and dissemination of the integron pool. ISME Journal 8:768-777.
  28. Tsegahun, A., Letemichael, N., Amlsha, K. and Yemane, W. (2017). Antibiotic Resistant Bacteria from treated and untreated Hospital Wastewater at Ayder Referral Hospital, Mekelle, North Ethiopia. Advances in Microbiology 7: 871-886.
  29. Williams, M.R., Stedtfeld, R.D., Guo, X. and Hashsham, S.A. (2016). Antimicrobial resistance in the environment. Water Environmental Research 88:1951-1967.
  30. Youngho, A. and Choi, J. (2016). Bacterial Communities and Antibiotic Resistance Communities in a Full-Scale Hospital Wastewater Treatment Plant by High-Throughput Pyrosequencing. Water 7: 580-591.
How to Cite

S, O. I., & O., O. P. (2020). Antibiotics Resistance in Haemolytic Bacterial pathogens from Hospital Wastewaters. Nigerian Journal of Microbiology, 34(1), 4945-4953. https://doi.org/10.67614/njm.2020.kt47ahjo

O. I. S, and O. P. O., "Antibiotics Resistance in Haemolytic Bacterial pathogens from Hospital Wastewaters," Nigerian Journal of Microbiology, vol. 34, no. 1, pp. 4945-4953, June 2020. doi: 10.67614/njm.2020.kt47ahjo

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