Bioethanol Production from Bitter Yam (Dioscorea dumetorum) and Water Yam (Dioscorea alata) Peels
1 Department of Biological sciences, Crawford University, PMB 2001, Igbesa, Ogun State, Nigeria.
2 Department of Biological sciences, Wellspring University, PMB 1230, Benin City, Edo State, Nigeria
3 Institute for Human Resources Development, Federal University of Agriculture, P.M.B 2240, Abeokuta, Ogun State, Nigeria.
* Corresponding author: topebanjo4rever@gmail.com
2 Department of Biological sciences, Wellspring University, PMB 1230, Benin City, Edo State, Nigeria
3 Institute for Human Resources Development, Federal University of Agriculture, P.M.B 2240, Abeokuta, Ogun State, Nigeria.
* Corresponding author: topebanjo4rever@gmail.com
Abstract
Bioethanol which is an alternative source of fuel to fossil fuels can be produced from renewable crops. However, some of these renewable feedstocks are food competitive. Hence, this study therefore investigated the production of bioethanol from bitter yam and water yam peels. Strains of Aspergillus spp and Saccharomyces cerevisiae were obtained from the Microbiology laboratory of the University and characterized using morphological characteristics. The spores of Aspergillus tamarii and colonies of Saccharomyces cerevisiae were cultured in bitter yam and water yam medium using the bitter and water yam peels as substrates for ethanol production at substrate concentrations of 5 – 30 %, temperature range of 25 - 45 oC, agitation speed of 60-160 rev/min and pH range of 4.0 - 8.0. Optimum bioethanol yield of 13 % was obtained with bitter yam peels at substrate concentration of 20 %, temperature of 35 oC, agitation of 100 rev/min and pH of 7.0. Similarly, optimum bioethanol yield of 11 % was obtained with water yam peels at substrate concentration of 20 %, temperature of 35 oC, agitation of 100 rev/min and pH of 5.0. Bioethanol yield from bitter yam peels was observed to be higher than that of water yam peels at substrate concentration of 20 %, temperature of 35 oC, agitation of 100 rev/min and pH of 7.0. This study shows the potential of bitter yam and water yam peels as substrates for the biosynthesis of ethanol which can serve as alternative source of fuel
Keywords
Aspergillus tamarii; Bioethanol; Bitter yam peels; Saccharomyces cerevisiae; Water yam peels
References
- Aliberti, A., Ventorino, V., Robertiello, A., Galasso, M., Blaiotta, G., Comite, E., Vonzeca, F. and Pepe, O. (2017). Configuration processions on cellulosic ethanol production from pretreated Arundo donax. Bioresources 12 (3): 5321-5342.
- Aloxie, Y., Akpanabiatu, M.I., Eyong, E.U., Umoh, I.B. and Alaxie, G. (2009). Amino acid composition of Dioscorea dumetorum varieties. Pak. J. Nutr. 8:103-105.
- Alvira, P., Tomás-Pejó, E., Ballestros, M. and Negro, M. J. (2010). Pretreatment technologies for an efficient bioethanol production process based on enzymatic hydrolysis: A review. Bioresource Technology 101(13)4851-4861.
- Caldwell, T., Publicover, K. and Harcum, S.W. (2010). Biofuel ethanol from using Saccharomyces bayanus, the champagne yeast. In Proceedings of the 32nd Symposium on Biotechnology for Fuels and Chemicals, Clearwater Beach. Pp. 11-34.
- Darvishi, F. and Moghaddami, N.A. (2019). Optimization of an Industrial Medium for Bioethanol Production Using the Taguchi Statistical Experimental-Design Method. Fermentation 5(1): 14; https://doi.org/10.3390/fermentation5010014
- Degras, L. and Coste, R. (1993). The Yam: A Tropical Root Crop. Macmillan, Netherlands. P.408.
- Dike, I.P., Obembe, O.O. and Adebiyi, E.F. (2012). Ethnobotanical survey of potential anti-malarial plants in South-western Nigeria. J. Ethnopharmacol. 144: 618-626.
- Fasaanu, O. P., Oziegbe, M. and Oyedapo, O. O. (2013). Investigations of activities of alkaloid of trifoliate yam (Dioscorea dumetorum, (kunth) pax. Ife Journal of Science 15(2): 251-262.
- Hashem, M., Zohri, A.N.A. and Maysa, M.A.A. (2013). Optimization of the fermentation conditions for ethanol production by new thermotolerant yeast strains of Kluyveromyces sp. African Journal of Microbiology Research 7(37): 4550-4561.
- Ibeto, C.N, Ofoefule, A.U. and Agbo, K.E. (2011). A Global Overview of Biomass Potentials for Bioethanol Production: A Renewable Alternative Fuel. Trends in Applied Sciences Research 6: 410-425.
- Iqbal, H.M.N., Asgher, M., Ahmed, I. and Hussain, S. (2010). Media optimization for hyper-production of carboxymethyl cellulase using proximally analyzed agro-industrial residues with Trichoderma harzianum under SSF. International Journal for Agro Veterinary and Medical Sciences (2): 47-55.
- James, C.S. (1995). Analytical chemistry of foods. Blackie Academic and Professional, London. P. 178.
- Jimenez, R.P., Pena, C., Ramirez, O.T. and Anandapandian, K.T.K. (2005). Specific growth rate determines the molecule mass of the alginate produced by Azotobacter vinelandii. Biochem. Eng. J. 25: 187-193.
- Kareem, S.O., Akpan, I and Oduntan, S.B. (2009). Cow pea waste: A novel substrate for solid waste production of amylase by Aspergillus oryzae. African Journal of Microbiological Research 3(12): 974-977.
- Kempf, M., Theobald, U. and Fiedler, H. P. (1997). Influence of dissolved O2 on the fermentative production of gallidermin by Staphylococcus gallinarum. Biotechnol. Lett. 19: 1063-1065.
- Kim, S. and Dele, E. (2005). Global potential Biothanol production from wasted crop and crop residue. Biomass Bioenergy 26: 361-367.
- Limtong, S., Sringview, C. and Yongmanitchai, W. (2007). Production of bioethanol at high temperature from sugar cane juice by a newly isolated Kluveromyces marxianus. Bioresource Technology Techniques 1(2): 123-126.
- Mahaseth, A.M. and Stewart, D.J. (1980). A medium for detecting beta-1,3-glucanase activity in bacteria. Journal of Applied Bacteriology 48: 457-458.
- Mazmanci, M.A. (2011). Ethanol production from Washingtonia robusta fruits by using commercial yeast. African Journal of Biotechnology 10(1): 48-53.
- Medoua, G.N, Mbome, I.L., Agbor-Egbe, T. and Mbofung, C.M.F. (2005). Study of the hard-to-cook property of stored yam tubers (Dioscorea dumetorum) and some determining biochemical factors. Food Research International 38:143-149.
- Narendranath NV (2001). Acetic and Lactic acid inhibition of growth by Saccharomyces cerevisiae
- Obianwa, C., Uyoh, E. and Igile, G. O. (2016). Bioethanol production from cassava peels using different microbial inoculants. African Journal of Biotechnology 15 (30):1608-1612.
- Ofoefule, A.U., Ibeto, C.N., Uzoma, C.C. and Oparaku, O.U. (2009). A key driver for improving climate change and socio-economic life in Nigeria. Int. J. Environ. Sci. 5: 54-58.
- Owuamanam, C.I, Iwuoha, C.I., Onuegbu, N.C., Ogueke, C.C. and Nwosu, J.N. (2011). Quality characteristics of processed flours from trifoliate yam (Dioscorea dumetorum) as influenced by steeping and boiling in varying concentration of trona solution over time. American Journal of Food Technology 8:162-172.
- Oyeleke, S.B. and Jibrin, N.M. (2009). Production of bioethanol from guinea cornhusk and millet husk. African Journal of Microbiology Research 3(4): 147-152.
- Rai, P., Tiwari, S. and Guar, R. (2012). Optimization of process parameters for cellulase production by novel thermotolerant yeast. Bioresources 7(4):5401-5414.
- Rodmui, A., Kongkiattikajorn, J. and Dandustapun, Y. (2008). Optimization of Agitation Conditions for Maximum Ethanol Production by Coculture. Kasetsart J. Nat. Sci. 42: 285 – 293.
- Saklani, S., Kothiyal, S.C. and Mishra, A. (2013). Nutritional profile, antinutritional profile and phytochemical screening of Garhwal Himalaya medicinal plant Dioscorea Alata tuber. Int. J. of Pharm. Sci. Rev. and Res. 23(2):42-46.
- Shankar, T., Kumar, R.S. and Ramirez, O.T. and Anandapandian, K.T.K. (2015). Statistical optimization for ethanol production by Saccharomyces cerevisiae 170) using response surface methodology. J. Adv. Med. Life Sci. 2: 1-5.
- Techapun, C., Poosaran, N., Watanabe, M. and Sasaki, K. (2003) Optimization of aeration and agitation rates to improve cellulose-free xylanase production by Streptomyces Spp. Ab106 and repeated fed batch cultivation using agricultural waste. J. Biosci. Bioeng. 95: 298-301.
- Tofighi, A., Azin, M., Mazaheri, A.M., Assadi, M.H.A., Nejadsattari, T. and Fallahian, M.R. (2011). Inhibitory effect of high concentrations of Furfural on industrial strain of Saccharomyces cerevisiae. Int J. Environ Res. 4(1):137–142.
- Wanasundera, J.P., Ravindran, G. (1994). Nutritional assessment of yam (Dioscorea alata) tubers. Plant Foods Human Nutrition 46(1): 33-39.
- Yan, S., Chen, X., Wu J. and Wang, P. (2012). Ethanol production from concentrated food waste hydrolysates with yeast cells immobilized on corn stalk. Applied Microbiology and Biotechnology. 94(3): 829-838.
How to Cite
T.T., B., C.B., O., T.O., B., & O.I, E. (2019). Bioethanol Production from Bitter Yam (Dioscorea dumetorum) and Water Yam (Dioscorea alata) Peels. Nigerian Journal of Microbiology, 33(2), 4687-4696.
B. T.T., O. C.B., B. T.O., and E. O.I, "Bioethanol Production from Bitter Yam (Dioscorea dumetorum) and Water Yam (Dioscorea alata) Peels," Nigerian Journal of Microbiology, vol. 33, no. 2, pp. 4687-4696, December 2019.