Research Article

Determination of the Growth Rate and Susceptibility Pattern of Fungi Using Agro Waste Formulated Media

Okoye E. L.1*, Uba B. O.2, Ugwuoke C. J2
1 Department of Applied Microbiology and Brewing, Nnamdi Azikiwe University, P.M.B. 5025 Awka, Anambra State, Nigeria
2 Department of Microbiology, Chukwuemeka Odumegwu Ojukwu University, P.M.B.02 Uli, Anambra State, Nigeria.
* Corresponding author: ebyemma2006@gmail.com
Published: Dec, 2020
DOI: 10.67614/njm.2020.9xgpeepy
Pages: 5258-5268

Abstract

Management of wastes especially agro wastes has been a major problem experienced in  developed and developing countries. There is a serious need to convert these agro-wastes into useful  forms. This study was undertaken to determine the growth rate and susceptibility pattern of fungi using  agro-waste formulated media. Sweet Potato and Watermelon peel wastes were used to formulate SPDA  and WDA media respectively and their performance compared with Potato Dextrose agar (PDA). The  proximate analysis of the peel wastes was done using standard methods. The methods used for fungi  isolation, cultivation and susceptibility testing were spread plate, agar plug diffusion and agar well  diffusion techniques respectively. Solvent extraction of scent leaf and Ginger was done using ethanol and  the extracts were used for the susceptibility testing. The result of the proximate analysis of the agro wastes  revealed the presence of carbohydrate, protein, crude fibre, moisture and ash. Nine fungal species were  isolated and used for growth rate and susceptibility testing. The result showed that PDA (0.14- 0.44h-1)  performed better than the formulated media (0.14- 0.18h-1 and 0.13-0.22h-1)in growth rate of the isolates  with a significant p value of 0.0020, while the formulated media(14- 41mm and 14-34mm; 0-25mm and 0 25mm) performed far better than the PDA(0-15mm and 0-16mm)in susceptibility testing with p values of  0.0028 and 0.0007 for Scent leaf ethanol extract and Ginger ethanol extract respectively. Thus the  formulated media can be utilized as alternative cheap sources of culture media for antifungal screening and  other mycological assays.

Keywords

Agro-waste Susceptibility testing Sweet potato peeldextrose agar Waste management and Watermelon peel dextrose agar

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How to Cite

L., O. E., O., U. B., & J, U. C. (2020). Determination of the Growth Rate and Susceptibility Pattern of Fungi Using Agro Waste Formulated Media. Nigerian Journal of Microbiology, 34(2), 5258-5268. https://doi.org/10.67614/njm.2020.9xgpeepy

O. E. L., U. B. O., and U. C. J, "Determination of the Growth Rate and Susceptibility Pattern of Fungi Using Agro Waste Formulated Media," Nigerian Journal of Microbiology, vol. 34, no. 2, pp. 5258-5268, December 2020. doi: 10.67614/njm.2020.9xgpeepy

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