Research Article

ERIC-PCR and Protein Patterns of Staphylococcus aureus isolated from Nigerian HIV-Positive Patients Admitted in different Hospitals in Imo State Nigeria

Ijeoma Emeka-Nwabunnia1*, Francisca O. Nwaokorie2, Viviane Nakano3, Mario J. Avila-Campos3, Ikechukwu N. E. Onwurah4
1 Department of Biotechnology, Federal University of Technology, Owerri, Nigeria
2 Division of Molecular Biology and Biotechnology, Nigerian Institute of Medical Research, Yaba, Lagos
3 Department of Microbiology, Institute of Biomedical Science, University of Sao Paulo, SP, Brazil
4 Department of Biochemistry, University of Nigeria, Nsukka
* Corresponding author: olaedo_k@yahoo.com
Published: Jun, 2018
DOI: 10.67614/njm.2018.dcfrdf8k
Pages: 4207-4305

Abstract

Opportunistic infections due to endogenous microorganisms have been a global problem in immune compromised Human Immunodeficiency Virus (HIV) positive patients. Staphylococcus aureus is a significant opportunistic pathogen among HIV patients in both nosocomial and community settings. There is scarce information on the relatedness of S. aureus strains isolated from HIV patients in Nigeria. The goal of the present study was to determine the genetic relatedness of S. aureus isolated from HIV-positive patients from three different hospitals in Imo State, Nigeria. Fifty nine S.. aureus strains obtained from HIV.positive patients attending Heajt to Heart centres at Owerri General Hospital, Okigwe General Hospital and Awo-Omama General Hospital all in Imo State, Nigeria, and presenting symptoms of pneumonia, oral thrush, skin and urinary tract infections, were subjected to enterobacterial repetitive intergenic consensus (ERIC)-PCR analysis and protein profiling to assess their genetic variability. Distinct 12 clusters with a clone circulating in two different hospitals were obtained. The strains in few clusters represent a clone because they are 100% related and therefore could be said to be of the same origin The protein profile showed different band sizes ranging from 26.5 to 300 kDa of which few strains from the three different hospitals were related. Inherent variations among S. aureus were observed and it could be due to the selective pressure produced by antibiotics used as part the treatment regime for these patients. The methods used afforded a valuable contribution in defining inherent variations among strains which would be useful in understanding the epidemiology and in designing prevention and control measures against the infections caused by this pathogen in HIV patients.

Keywords

ERIC-PCR protein profiles HIV Staphylococcus aureus

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How to Cite

Emeka-Nwabunnia, I., Nwaokorie, F. O., Nakano, V., Avila-Campos, M. J., & Onwurah, I. N. E. (2018). ERIC-PCR and Protein Patterns of Staphylococcus aureus isolated from Nigerian HIV-Positive Patients Admitted in different Hospitals in Imo State Nigeria. Nigerian Journal of Microbiology, 32(1), 4207-4305. https://doi.org/10.67614/njm.2018.dcfrdf8k

I. Emeka-Nwabunnia, F. O. Nwaokorie, V. Nakano, M. J. Avila-Campos, and I. N. E. Onwurah, "ERIC-PCR and Protein Patterns of Staphylococcus aureus isolated from Nigerian HIV-Positive Patients Admitted in different Hospitals in Imo State Nigeria," Nigerian Journal of Microbiology, vol. 32, no. 1, pp. 4207-4305, June 2018. doi: 10.67614/njm.2018.dcfrdf8k

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