Research Article

Escherichia coli O157:H7 Contamination of Cattle Carcass at Slaughter from Abattoirs in Lagos, Nigeria

Uzeh Roseline Ekiomado1*, Agunlanna Stephanie Chineye1
1 Department of Microbiology, Faculty of Science, University of Lagos, Lagos, Nigeria
* Corresponding author: roseline.uzeh@gmail.com
Published: Dec, 2019
Pages: 4803-4810

Abstract

Meat obtained from cattle serve as a major source of protein in Nigeria, ensuring its safety will therefore be of great importance. This study was carried out to investigate the contamination of cattle carcass with E. coli O157:H7 and determine the antimicrobial susceptibility of the organism. Twenty meat samples from bowel, aitch bone, hide, rib and hunch back were collected from abattoirs in Lagos, Nigeria. MacConkey agar and Eosine methylene blue agar were used for isolation of E. coli. Isolates were sub-cultured on sorbitol MacConkey agar and E. coli O157:H7 were differentiated from other strains due to its inability to ferment sorbitol. Identification was based on cultural, morphological and biochemical characteristics. Susceptibility of the isolates to amoxicillin, ceftazidime and cefuroxime was also investigated. All E. coli isolates were catalase and indole positive, oxidase, citrate and urease negative. The mean total coliform count varied from 4.45x107 cfu/g (hunch back) to 9.20x107 cfu/g (bowel). E. coli O157:H7 occurred in 37% of the meat samples while other E. coli strains occurred in 63%. They both occurred in meat samples from the different parts of the carcass investigated. Among the fourteen E. coli O157:H7 isolates 57.14% were resistant to ceftazidime, 42.86% to cefuroxime and 78.57% to amoxicillin. E. coli, although an enteric bacterium, was found to contaminate different parts of cattle carcass as a result of slaughtering. Of greater concern was the antibiotic resistance and isolation of the pathogenic strain, E. coli O157:H7.

Keywords

Escherichia coli O157:H7 cattle carcass antibiotic resistance

References

  1. Ajayi, I.A., Ajibade, O. and Oderinde, R.A. (2011). Preliminaryphytochemical analysis of some plant seeds. Research Journal of Chemical and Pharmaceutical Sciences, 4(2):58-62.
  2. Ameen, O.M., Olatunji, G.A., Atata, R.F., Usman, L.A. (2010). Antimicrobial activity, cytotoxic test and phytochemical screening of extracts of the stem of Fadogiaagrestis. Nigerian Journal of Pharmaceutical Sciences, 15(2): 25-30.
  3. Anowi, C.F., Cardinal, N.C., Ezugwu, C.O. and Utoh-Nedosa, U.A. (2012). Antimicrobial properties of the chloroform extract of the stem bark of Nauclea latifolia. International Journal of Pharmacy and Pharmaceutical Sciences, 4(2): 744-750.
  4. Atukpawu, C.P. and Ozoh, P.T.E. (2014). Antimicrobial studies of aqueous and ethanolic extracts of Enantia chlorantha leave and stem bark and their combined effect on selected bacteria and fungi. European Journal of Medicinal Plants, 4(9): 1036-1045.
  5. Babayi, H., Kolo, I., Okogun, J. and Ijah, U. (2004). The antimicrobial activities of methanolic extracts of Eucalyptus camaldulensis and Terminalia catappa against some pathogenic microorganism.Biochemistry, 16(2): 106- 111.
  6. Clinical and Laboratory Standards Institute, (2007).
  7. Ejikeugwu, C., Umeokoli, B., Iroha, I., Ugwu,M. and Esimone, C. (2014). Phytochemical Screening of Crude Extracts from Leaves of Wonderful Kola. American Journal of Life sciences, 2(6-3): 9-12.
  8. Esimone, C.O., Adikwu, M.U.andOkonta, J.M. (1998). Preliminary antimicrobial screening of the ethanolic extract from the lichen Usneasub floridans. Journal of Pharmaceutical Development, 3(2): 99-101.
  9. Esimone, C.O., Akah, P.A. and Nworu, C.S. (2009). Efficacy and Safety Assessment of T. angelica Herbal Tonic, a Phytomedicinal product popularly used in Nigeria. Evidence – Based Complementary and Alternative Medicine.1-6.
  10. Ezeja, M.I., Ezeigbo, I.I. and Madubuike, K.G. (2011) Analgesic activity of the methanolic seed extract of Buchholzia coriacea. Research Journal of Pharmaceutical, Biological and chemical sciences, 2:187-193.
  11. Falodun, A., Nworgu, Z.A.M. and Ikponmwosa, M.O. (2006) Phytochemical components of Hunteria umbellate (K.schum) and its effect on isolated non-pregnant rat uterus in oestrus. Pakistan Journal of Pharmaceutical Sciences, 19(3): 256-258.
  12. Ibrahim, T.A. and Fagbohum, E.D. (2014). Phytochemical and Nutritive Qualities of dried seeds of Buchholzia coriacea. Research and Review: Journal of food and Dairy Technology 2(2): 2.
  13. Junaid, S.A., Olabode, A.O., Onwuliri, F.C., Okowsi, A.E.J. and Agina, S.E. (2006). The antimicrobial properties of Ocimum gratisimum extracts on some elected bacterial gastrointestinal isolate. African Journal of Biotechnology, 5 (22): 2315-2321
  14. Kareem, K. T., Kareem, S.O., Adeyemo, O. J and Egbewande, R. K. (2010). In vitro antimicrobial properties of Bridelia ferruginea on some clinical isolates.Agriculture and Biology Journal of North America, 1(3):416-420.
  15. Lemmens, J.S. (2015). The internet gaming disorder scale. Journal of behavioral addiction, 2(2): 567-582.
  16. Mbata, T.I., Duru,C.M.and Onwumelu, H.A.(2009). Antibacterial activity of crude seed extracts of Burkholzia coriacea on some pathogenic bacteria. Journal of Developmental Biology and Tissue Engineering, 2(1): 1-5.
  17. Nwachukwu, M.I., Ozo, M.K.C, Amadi, B.A. and Nwachukwu, I.O. (2011). Comparative evaluation of phytoconstituent, antibacterial activities and proximate contents of fresh, oven dried uncooked and cooked samples of Buchholzia coriacea seed and their effects on Hepatocellular integrity. International Journal of Pharmaceutical Sciences Invention. 3(61):41-49.
  18. Nweze, N.E. (2011). Studies on the antioxidant and antimicrobialactivities of the seed extracts of Buchholzia coriacea (Capparaceae). Nigerian Veterinary Journal, 32(2):143-147.
  19. Olusey, E.O. and Onyeozori, N.F. (2009).Preliminary studies on theantimicrobial properties of Buchholzia coriacea (wonderfulkola).African Journal of Biotechnology, 8(3):472-474.
  20. Osadebe, P.O. and Ukwueze, S.E. (2004). Comparative study of the phytochemical and antimicrobial properties of the Eastern Nigerian species of African Mistletoe (Loranthusmicranthus) sourced from different host trees. Journal of Biological Research Biotechnology, 5 (2): 18-23
  21. Shagal, M.H, Modibbo, U.U. and Liman, A.B. (2012). Pharmacological justification for the ethnomedical use of Daturastramonium stem-bark in treatment of diseases caused by some pathogenic bacteria. International Journal of Research and Pharmacology, 2(1): 16-19.
  22. Silver, O., Duarte, A., Cabrita, J., Pimentel, M., Diniz, A. and Gomes, E. (1997) Antimicrobial activity of Guinea Bissau traditional remedies. Journal of Ethnopharmacology, 2:55-59.
  23. Trease, G.E. and Evans, W.C. (1999) Pharmacology, 13th edition Baciliere Tindal, London, 60-75.
How to Cite

Ekiomado, U. R., & Chineye, A. S. (2019). Escherichia coli O157:H7 Contamination of Cattle Carcass at Slaughter from Abattoirs in Lagos, Nigeria. Nigerian Journal of Microbiology, 33(2), 4803-4810.

U. R. Ekiomado, and A. S. Chineye, "Escherichia coli O157:H7 Contamination of Cattle Carcass at Slaughter from Abattoirs in Lagos, Nigeria," Nigerian Journal of Microbiology, vol. 33, no. 2, pp. 4803-4810, December 2019.

Share this article:
Facebook X / Twitter LinkedIn
Download PDF View Full Text (PDF) Back to Articles