Evaluation of Antibacterial Activity of Stem Bark Extracts of Diospyros mespiliformis and Boswellia serrata against Some Clinical Bacterial Isolates Obtained from Haemorrhoid Lesions
1 Department of Microbiology, Bayero University, Kano, Nigeria.
2 Department of Microbiology, Bayero University Kano, BUK, P.M.B 3011, Nigeria
* Corresponding author: bshchemiron@yahoo.com
2 Department of Microbiology, Bayero University Kano, BUK, P.M.B 3011, Nigeria
* Corresponding author: bshchemiron@yahoo.com
Abstract
Bacterial resistance to antibiotics is one of the major problems encountered in the field of chemotherapy worldwide. Some pathogenic bacteria are becoming resistant to most potent antibiotics used in hospitals and clinics; this necessitate for the search of new and promising antibacterial agents. In view of that, the antibacterial activity of stem bark extracts of D. mespiliformis and B. serrata locally used for the treatment of haemorrhoids against bacterial isolates sourced from haemorrhoid lesions in patients attending Aminu Kano Teaching and Murtala Muhammad Specialist Hospitals, Kano was investigated. Aqueous and ethanolic extracts of the stem bark were obtained and screened for phytochemical constituents using cold maceration technique. Swabs were aseptically collected using sterile swabsticks and screened for bacterial pathogens. Isolates were identified using cultural, biochemical and molecular techniques. Antibacterial activity of low and high concentrations of the extracts was determined using agar well diffusion technique. The results of the phytochemical screening of the extracts revealed the presence of phenols, cardiac glycosides, steroids, flavonoids, tannins and anthraquinones in the extracts. The bacterial species identified were Enteropathogenic Escherichia coli, Proteus vulgaris, Proteus mirabilis, Enterobacter agglomerans, Enterobacter aerogenes, Salmonella arizonae, Salmonella enterica, Salmonella Typhi, Shigellla dysentriae and Citrobacter freundii. The results of the antibacterial activity of the extracts revealed low activity of both the low and high concentrations of the aqueous and ethanolic extracts of the two plants against the test organisms; zones diameter of inhibition ranging from 6.00-12.45mm were recorded; the well diameter was 6mm. In conclusion, the crude stem bark extracts of the two plants possessed weak antibacterial activity. It is recommended that other plants’ parts should be tested for antibacterial activity.
Keywords
Phytochemical screening
Antibacterial activity
Clinical isolates
Agar well diffusion technique
References
- Abdullahi, A. E., Dahiru, S., Mohammed, M. S., Abubakar, A. and Adamu, Z. H. (2019). Phytochemical and Antimicrobial Screening of the Methanolic Extracts of D. mespiliformis Hochst. Pharmacognosy Journal, 11 (2):362-368.
- Abdulrahman, B., Mammam, M., Suleiman, M. M., Ahmed, A. and Bello, A. (2014). Preliminary Phytochemical Screening of D. mespiliformis. The Journal of Agriculture and Natural Resources Sciences, 1 (4):12-8.
- Adeoye, A. (2007). A Textbook for Medical Laboratory Practice. First Edition, Distributed by Edoson Publishers a Division of Argus Edition (Nig.) Ltd. Pp 117-156.
- Ahmed, A. H. and Mahmud, A. F. (2017). Pharmacological Activities of D. mespiliformis. A Review. Int. Journal of Pharmacy and Biological Sciences, 7(4):93-96.
- Burkill, H. M. (1995). The Useful Plants of West Tropical Africa. Volume 1. Pp 200 – 209.
- Cheesbrough, M. (2006). Disraica laboratory practice in tropical countries. Part 1, second edition, Pp. 12 – 17.
- Clinical and Laboratory Standards Institute (CLSI) (2012). Performance Standards for Antimicrobial Susceptibility Testing: Approved standard- 4. Edition. M02-A11. Vol 32(1): 1 – 200.
- Dangogo, S. M., Hassan, L. G., Sadiq, T. T. and Manga, S. B. (2016). Phytochemical Analyses and Antibacterial Screening of Leaf of D. mespiliformis and Ziziphus spin-Christi. Journal of Biochemistry Research and Review, 12 (4):1-9.
- Lee, C. R., Lee, J. H., Park, K. S., Kim, Y. B., Jeong, B. C and Lee, S. H. (2016). Global Dissemination of Carbapenemase Producing K. pneumoniae Epidemiology, Genetic Context Treatment Option and Detection Methods. Microbiol,7:895-900.
- Mansur, I. S, Sudheer, A, Sambasivarao, K., and Matcha, B. (2014). Antimicrobial Activity of Frankincense of B. serraia. International Journal of Current Microbiology and Applied Sciences, 3 (19): 1095 -1101.
- Sara, N. J.W. and Ikram, M. E. (2018). A Comparative Study of Anti-inflammatoryand Chemical Analysis of Ziziphus spina-Christi Leaves and B. serraia Gum Dry Distillates. 6th Edition of Int. Conference on Pharmacognosy and Medicinal Plants Amsterdam, Netherlands.
- Shagal, M. H., Kubmarawa, D. and Alim, H. (2011). Preliminary Phytochemical Investigation and Antimicrobial Evaluation of Roots, Stem bark and Leaves Extracts of D. mespiliformis. International Research Journal of Biochemistry and Bioinformatics, 2 (5):011-015.
- Siddiqui, M. Z. (2011). Antiinflammatory Activity Resins from B. serraia. Indian Journal of Pharm. Sciences, 73 (3):255-261.
- Sofowora, A. (1993): Medicinal plants and Traditional medicine in Africa, Spectrum Book LTD, Ibadan. Nigeria: 289.
- Yadima, S. G., Murtala, M and Bello, A. U. (2017). Phytochemical Screening and Antibacterial Activities of Aqueous Extracts of D. mespiliformis, Miiragyna reticulaum and Tamarindus indica in Northern Nigeria. Arid Journal of Engineering, Technology and Environment, 13 (3): 420-427.
How to Cite
B., M., & U.I., D. (2020). Evaluation of Antibacterial Activity of Stem Bark Extracts of Diospyros mespiliformis and Boswellia serrata against Some Clinical Bacterial Isolates Obtained from Haemorrhoid Lesions. Nigerian Journal of Microbiology, 34(1), 5080-5086. https://doi.org/10.67614/njm.2020.m9wrraez
M. B., and D. U.I., "Evaluation of Antibacterial Activity of Stem Bark Extracts of Diospyros mespiliformis and Boswellia serrata against Some Clinical Bacterial Isolates Obtained from Haemorrhoid Lesions," Nigerian Journal of Microbiology, vol. 34, no. 1, pp. 5080-5086, June 2020. doi: 10.67614/njm.2020.m9wrraez
