Research Article

Isolation and Identification of Coliform Bacteria from Selected Well Water in Lapai Metropolis, Niger State, Nigeria

Baba, J.1*, Majiya, H. M1, Muhammad, I. L2, Abdullahi, M3, Oladoke, A.B.3
1 Department of Microbiology, Ibrahim Badamasi Babangida University, Lapai.
2 Department of Microbiology, Ibrahim Badamasi Babangida University, Lapai
3 Department of Science Lab. Tech., Niger State Polytechnic, Nigeria..
* Corresponding author: babajohn200133@yahoo.co.uk
Published: Jun, 2018
Pages: 4087-4091

Abstract

The study was carried out to isolate and identify Coliform bacteria from well water situated in Lapai metropolis. Fifteen (15)samples of well water, used for domestic purposes were collected from different well water points within Lapai metropolis. Most Probable Number was used for enumeration of Coliform, Cultured on Nutrient agar and Eosin methylene blue (EMB) Agar. The isolates were subjected to series of biochemical tests for possible identification. The physicochemical parameters analyzed were pH, Temperature, and Turbidity. The MPN coliform index per 100ml of the water samples ranged from 0-1100+. The Bacteria isolated from well water samples included, Escherichia coli (26.7 %,) Citrobacter sp (13.3%), Shigella sp (13.3%), Salmonella sp(6.7%), Klebsiella sp (20.0%), Serratia sp (13.3%), and Enterobacter sp(6.7%). The percentage occurrence of the isolates indicated that E. coli was predominant with 26.7%, the least occurrence of the isolates were Salmonella sp, and Enterobacter . sp with 6.7% each. The highest temperature recorded for the samples was 25.8°C, and the least was 20.7°C. The pH of the water samples ranged from 6.48-8.15. The turbidity values of the well water samples ranged from 0-4 Nephelometric Turbidity Units (NTU). From the results obtained, most of the water samples analyzed met World Health Organization standard of 0 MPN index/100ml. However, the few samples observed to harbour contaminations calls for treatment before consumption.

Keywords

Isolation Coliform bacteria Well water

References

  1. Abera Solomon, Zeyinudin Ahmed, Birukrawit Kebede, Deribew Amare, Ali Solomon and Zemene Endalew (2011). Bacteriological analysis of Drinking water sources. African Journal of Microbiology research, Vol. 5(18): pp2638- 2641.
  2. Amira, A.A and Yassir, M. E. (2011). Bacteriological quality of drinking water in Nyala, South Darfur, Sudan. Environ. Monit. Assess, 175: 37-43,
  3. APHA (American Public Health Association) (2005). Standard methods for the examination of water and wastewater,21th edition. Washington DC. pp 114-117
  4. Chan, C. L., Zalifah, M. K. and Norrakiah, A. S. (2007) Microbiological and Physicochemical Quality of Drinking Water. The Malaysian Journal of Analytical Science,11(2): 414-420.
  5. Cheesborough, M (2006). District Laboratory Practice in Tropical Countries. Part 2. Second Edition, Cambridge University press, London. pp 132-194.
  6. Gerald, T, H., Pruss, A. (1992). "Review of epidemiological studies on health effects from exposure to recreational water". International Journal of Epidemiology,27 (1): 1-9.
  7. Hanna R., Ejobi, F and Kabagambe, E.K. (2005). The quality of water from protected springs in Katwe and Kisenyi parishes, Kampala City, Uganda. Afri. Health Sci., 5(1) : 14-20.
  8. Kravitz, J. D., Nyaphisi , M., Mandel, R and Petersen E (1999). Quantitative bacterial examination of domestic water supplies in the Lesotho Highlands: Water quality. Sanitation and Village Health. World Health Organ., 77: 829-836.
  9. Michael, J., Pelczar, J. E, Chan, G., Noel R and Krieg, F. (1993).Microbiology, 5th Edition,pp.596-601.
  10. Medera, P.A., Levesque, B., Simard, P., Gauvin, D., Gingrasl, S., Dewailly, E and Letarte, R. (1999). Comparison of the microbiological quality of water coolers and that of municipal water system. Journal of Applied Environmental Microbiology, 60(117): 4-1178.
  11. Mengesha, A., Mawo, W and Baye, G. (2004). A survey of bacteriological quality of drinking water in North Gondar, Ethiop. J. Health Dev., 18: 112-115
  12. Olayemi, B.A., Adekunle, I. M., Adekunle, M. T., Gbadebo, A. M and Banjoko, O. B. (2007).Assessment of Ground Water Quality in a typical Rural Settlement in Southwest Nigeria. Int. J. Environ. Res. Public Health,4(4): 307-318.
  13. Oyedeji, O., Olutiola, P. O and Moninuola, M. A. (2010). Microbiological quality of packaged drinking water brands marketed in Ibadan metropolis and Ile-Ife city in south western Nigeria. Afri. J. Microbiol. Res.,4(4): 96-102.
  14. Oyeleke S.B. and Manga B.S. (2008). Essentials of Laboratory practice's in Microbiology (1st Edition) Nigeria: TOBEST Publishers Pp 20-39.
  15. Payment, (1993). Australian environmental water management report. NWC, Canberra. Pp 2-4
  16. Ryan, K. T., Wolf, A. T. (2004) "Water and Human Security."Journal of Contemporary Water Research and Education , 29: 116-120.
  17. WHO (2006). Guidelines for Drinking – water Quality. Third Edition, Geneva Volume 1, pp2-7.
How to Cite

J., B., M, M. H., L, M. I., M, A., & A.B., O. (2018). Isolation and Identification of Coliform Bacteria from Selected Well Water in Lapai Metropolis, Niger State, Nigeria. Nigerian Journal of Microbiology, 32(1), 4087-4091.

B. J., M. H. M, M. I. L, A. M, and O. A.B., "Isolation and Identification of Coliform Bacteria from Selected Well Water in Lapai Metropolis, Niger State, Nigeria," Nigerian Journal of Microbiology, vol. 32, no. 1, pp. 4087-4091, June 2018.

Share this article:
Facebook X / Twitter LinkedIn
Download PDF Back to Articles