Research Article

Low Incidence of Extended Spectrum Beta Lactamase (ESBL) Detection From Enterobacteriaceae Isolated From Pig Faeces

Itaman, V.O1*, Nwankwo, E.O1, Erhunmwunse, A.S2, Adeleye, LA3
1 Department of Microbiology, Michael Okpara University of Agriculture, Umudike, Abia, Nigeria.
2 Department of Soil Science, Obafemi Awolowo University, lle-Ife, Nigeria
3 Department of Microbiology, University of Lagos, Lagos, Nigeria
* Corresponding author: itamanvivien@yahoo.com
Published: Dec, 2017
DOI: 10.67614/njm.2017.cl0n3mdd
Pages: 4063-4067

Abstract

This study assessed the low incidence of ESBL detect and associated antimicrobial/antibiotic resistance from Enterobacteriaceae isolated from pigs. A total of twenty-six (26) isolates from the forty-five (45) faecal piggery samples studied were Gram negative bacteria which were further characterized using standard biochemical procedures as : Citrobacter freundii 7(26.9%), E. coli 5(19.2%), Klebsiella pneumonia 5(19.2%), Enterobacter aerogenes 2(7.7%), Salmonella spp. 6(23.1%), and Shigella spp. 1(3.9%). Antimicrobial susceptibility of these isolates to the following antibiotics: gentamicin (GEN) lOug, cefuroxime (CRX) 30 ug, ofloxacin (OFL) 5 ug, ceftazidime (CAZ) 30 ug, augmentin (AUG) 30 ug, cefixime (CXM) 5 ug, nitrofurantin (NIT) 30 ug, ciprofloxacin (CPR) 5 ug was determined using the Kirby-Bauer disc diffusion method; twelve (12) of the isolates showed resistance to two or more of the antibiotics. The major finding is the presence of multi-drug resistant E.coli and Klebsiella pneumonia to commonly used antibiotics such as augmentin, ceftazidime, cefixime, and cefuroxime. These multi drug resistant isolates were screened for possible ESBLs production using the double disc synergy test (DDST) and none was ESBL positive. Pig faeces do not pose a high risk of contamination with ESBL producing bacteria of the Enterobacteriaceae group in the environment.

Keywords

ESBL Enterobacteriaceae pig faeces

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How to Cite

V.O, I., E.O, N., A.S, E., & LA, A. (2017). Low Incidence of Extended Spectrum Beta Lactamase (ESBL) Detection From Enterobacteriaceae Isolated From Pig Faeces. Nigerian Journal of Microbiology, 31(2), 4063-4067. https://doi.org/10.67614/njm.2017.cl0n3mdd

I. V.O, N. E.O, E. A.S, and A. LA, "Low Incidence of Extended Spectrum Beta Lactamase (ESBL) Detection From Enterobacteriaceae Isolated From Pig Faeces," Nigerian Journal of Microbiology, vol. 31, no. 2, pp. 4063-4067, December 2017. doi: 10.67614/njm.2017.cl0n3mdd

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