Research Article

Optimized Fermentation Process for Improved Bioethanol Production from Sweet and Bitter Cassava Processing Wastes

Obueh H.O1*, Ikenebomeh, M. J,2, Oshoma C. E3
1 Biology Department, College of Education, P. M. B 1144, Ekiadolor, Benin City, Edo State, Nigeria.
2 Microbiology Department, University of Benin, P. M. B 1154, Benin City, Edo State, Nigeria.
3 Department of Microbiology, Faculty of Life Sciences, University of Benin, P.M.B. 1154, Benin City, Edo State, Nigeria.
* Corresponding author: obuehho@nsmjournal.org.ng
Published: Jun, 2016
Pages: 3350-3357

Abstract

Optimization of fermentation process for 72 h was carried out to determine the effect of pH (3 – 8), temperature (30°C – 50°C) and agitation rate (200 rpm – 500 rpm) on bioethanol production and microbial count using cassava variety samples. Bioethanol yield and microbial count were highest at pH 6, temperature 35°C and agitation rate of 300 rpm. The bacterial species had highest population counts of 5. 21 x 105 cfu/g at pH 6, 4.62 x 105 cfu/g at temperature 35°C and 4.84 x 105 cfu/g at pH 6, 3.94 x 105 cfu/g at temperature 35 °C for sweet and bitter cassava varieties respectively. The fungal species had highest counts of 5.60 x 104 cfu/g at pH 6, 2.53 x 104 cfu/g at temperature 35°C and 5.20 x 104 cfu/g at pH 6, 2.11 x 104 cfu/g at temperature 35°C for sweet and bitter cassava varieties. Bioethanol yield was 56% and 44% resulting in a significant increase of 75% and 63% for the sweet and bitter cassava varieties respectively. The optimization of the fermentation process yielded maximum bioethanol and also detoxified the cassava processing wastes which are environmental pollutants.

Keywords

Bioethanol Bitter cassava Optimized fermentation process Processing Wastes Sweet Cassava

References

  1. Abodjo Kakou, C., Tago Guehi, S., Otio, K., Akissi Kouame, F., Fotti Nevry, R. and Marina Koussemon, C (2010) Biochemical and microbial changes during traditional spontaneous lactic acid fermentation process using two varieties of cassava for production of a "Alladjah" starter. International Food Research Journal 17:563 – 573.
  2. Ado, S. A., Olutokin G. B., Amen, J B and Yabaya, A (2009) Bioconversion of cassava starch to ethanol in a simultaneous saccharification and fermentation process by co – cultures of Aspergillus niger and Saccharomyces cerevisiae World Journal 4(1):19 – 22.
  3. Agbogbo F and Wenger, K (2007) Production of ethanol from corn stover hemicelluloses hydrolysate using Pichiastis. Journal of Industrial Microbiology and Biotechnology 34:723 – 737
  4. Akponah, E and Akpomie, O. O (2012) Optimization of bio-ethanol production from cassava effluents using Saccharomyces cerevisiae. African Journal of Biotechnology 11(32): 8116 – 8119.
  5. Amjj, K. C, Ravinder, R., Lakshmi, M N, Rao, V and Ravinder, P (2007) Economic and environmental impact of bioethanol production technology. Biotechnology Molecular Biology Review 32(1):14 – 32.
  6. AOAC (2000) Association of Official Analytical Chemists. Official Methods of Analysis 17th edition. The Association of Official Analytical Chemists. Arlington, Virginia, Gaithersburg, MD, USA.
  7. AOAC (2001) Bacteriological analytical manual online US Food and Drug Administration Washington DC P.946.
  8. Ballesteros, I., Olivn J. M., Navarro, A., Carrasco, J and Ballesteros, M (2000) Effect of chip size on steam explosion pretreatment of softwood. Applied Biochemistry and Biotechnology 84(86):97 – 110.
  9. Benaeji, D. N. S., Rajini, K., Srinivasa Rao, B., Banerjee, D. R. N., Swaroopa Rani, K., Rajkumar, G and Ayyanna, C (2010) Studies on physicochemical and nutritional parameters for the production of ethanol from malua flower (Madhuca indica) using Saccharomyces cerevisiae 3090 through submerged fermentation (smf). Journal of Microbial and Biochemical Technology 2(2): 46 - 50.
  10. Chiwona-Karltun, L., Mkumbira, J, Salca, J, Bovin, M., Muhungu, N. M., and Rosling, H (1998) The importance of being bitter - a qualitative study on cassava cultivar preference in Malawi. Ecology and Nutrition of Food 37: 219 – 245.
  11. Dixon, A. G, Ngeve, J. M., and Nukenine, E. N (2002) Response of cassava genotypes to four biotic constraints in three agro-ecologies of Nigeria. African Crop Science Journal 10:11 – 21.
  12. Ezeama, C. F (2007) Food microbiology: fundamentals and applications. Natural Prints Ltd. Lagos, Nigeria. P 66 – 72.
  13. Kimaryo, V. W., Massawi, G. A., Olasupo, N. A and Holzapfel, W H (2000) The use of a starter culture in the fermentation of cassava for the production of 'kivunde', a traditional Tanzanian food product. International Journal of Food Microbiology 56: 179 – 190.
  14. Klaenhammer, T. R (1993) Genetics of bacteriocins produced by lactic acid bacteria FEMS Microbiology 12:38 – 55.
  15. Kosogi, A., Kondo, A., Ueda, M, Murata, Y., Vaithanonsat, P., Thanapase, W., et al. (2009) Production of ethanol from steam-exploded bamboo by recombinant zymomonas mobilis expressing xylose utilization genes. Process Biochemistry 44(9): 1013 – 1019.
  16. Mori, T (2009): Production of ethanol from cassava pulp via fermentation with a surface-engineered yeast strain displaying glucoamylase. Renewable Energy 34:1354 – 1358.
  17. Lin, Y and Tanaka, S (2006) Ethanol fermentation from biomass resources: current state and prospects. Applied Microbiology and Biotechnology 69:627-642
  18. Maiorella, B. L., Wilke, C. R. and Blanch, H. W (1981) Alcohol production and recovery. Advanced Biochemistry and Engineering 20: 43.
  19. Miller, G. L (1959) Use of dinitrosalicylic acid reagent for the determination of reducing sugars. Analytical Chemistry 3: 426 – 428.
  20. Neelakandan, T and Usharani, G (2009) Optimization and production of bioethanol from cashew apple juice using immobilized yeast cells by Saccharomyces cerevisiae. American – Eurasian Journal of Scientific Research 4(2): 85 – 88
  21. Nuwamanya, E., Chiwona-Kaltun, L., Kawuki, R S and Baguma, Y (2011) Bio - ethanol production from Manihot esculenta Crantz: AMBIO Journal of Human Environment 10: 1 – 16.
  22. Obadina, A.O, Oyewole, O.B, Sanni, L.O and Abiola, S.S (2006) Fungal enrichment of cassava peels proteins. African Journal of Biotechnology 5 (3): 302 – 304.
  23. Oboh, G and Elusiyan, C. A (2007) Changes in the nutrient and anti-nutrient content of micro-fungi fermented cassava flour produced from low and medium cyanide variety of cassava tubers. African Journal of Biotechnology 6 (18): 2150 – 2157.
  24. Obueh, H O (2014) Bio energy production from cassava fractions and their processing wastes by naturally occurring microbial isolates. Ph. D Thesis University of Benin. UNIBEN Press P.268.
  25. Obueh, H O and Ikenebomeh, M J (2014) Biofuel production and compositional changes during fermentation of cassava processing wastes from a local cassava mill community. International Journal of Current Research in Bioscience and Plant Biology 1(4): 43 – 51
  26. Ocloo, F. C. K and Ayernor, G. S (2010) Production of alcohol from cassava flour hydrolyzate. Journal of Brewing and Distilling 2(1): 15 – 21.
  27. Ogbeibu, A. E (2005) Biostatistics: A practical approach to research and data handling. Mindex Publishing Co Ltd Benin City P 271.
  28. Olofsson, K., Bartilsson, M and Liden, G (2008) A short review on SSF: An interesting process option for ethanol production from lignocellulosic feed stock. Biotechnology of Biofuels 1(7):1 – 14.
  29. Oyeleke, S. B., Dauda, B. E. N., Oyewole, O. A., Okolibole, I N and Ojebode, T (2012) Production of bioethanol from cassava and sweet potato peels. Advances in Environmental Biology 6(1): 241 – 245
  30. Oyewole, O.B (1990) Cassava processing in fermentation for fufu production. Effects of single starter cultures. Journal of Applied Bacteriology 68: 49 – 54.
  31. Raimbault, M (1995) Importance des bacteries lactiques dans les fermentations du manioc. In Agbor Egbe, Brauman, Griffon, Treche, Transformation Alimentaire du Manioc. ed orestom, Paris P.747.
  32. Senthilkumar, P. K., Uma, C and Sarranj, P (2012) Amylase production by Bacillus sp using cassava as substrate. International Journal of Pharmaceutical Biological Sciences 3(2): 306 – 306.
  33. Techi, F. A., Solomon, O., Celah, F.A and Georges, A. N (2012) Effect of cassava variety and fermentation time on biochemical and microbiological characteristics of raw artisan starter for Attieke production. Innovative Rmanian Food Biotechnology 10 (3): 40 – 47.
  34. Tijjani, I. D. R., Jamal, P, Alam, M. Z and Mirghani, M. E. S (2012) Optimization of cassava peel medium to an enriched material feed for the white rot fungi Panus tigrinus M609RQY. International Food Research Journal 19(2):427 – 232.
  35. Ziska, L, Runion, G, Tomecek, M, Prior, S., Torbet, A and Sicher, R (2009). An Evaluation of cassava, sweet potato and field corn as potential carbohydrate sources for bioethanol production in Alabana and Maryland. Biomass and Bioenergy 33: 1503 – 1508.
How to Cite

H.O, O., J,, I. M., & E, O. C. (2016). Optimized Fermentation Process for Improved Bioethanol Production from Sweet and Bitter Cassava Processing Wastes. Nigerian Journal of Microbiology, 30(1), 3350-3357.

O. H.O, I. M. J,, and O. C. E, "Optimized Fermentation Process for Improved Bioethanol Production from Sweet and Bitter Cassava Processing Wastes," Nigerian Journal of Microbiology, vol. 30, no. 1, pp. 3350-3357, June 2016.

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