Research Article

Production of Bacteriocins by Lactobacillus plantarum and Pediococcus acidilactici Isolated from Cow Milk

Anumudu Christian K1*, Ikimi Charles G2, Zige Douye Victor1, Omeje Faith I1, Gbodo Edoama E1
1 Department of Microbiology, Federal University Otuoke, Bayelsa State Nigeria
2 Department of Biochemistry, Federal University Otuoke, Bayelsa State Nigeria
* Corresponding author: anumuduchristiank@nsmjournal.org.ng
Published: Jun, 2019
DOI: 10.67614/njm.2019.pqas4gd3
Pages: 4373 - 4379

Abstract

A dramatic increase in bacterial resistance towards currently available antibiotics has raised worldwide concerns for public health. Therefore, bacteriocins which are antimicrobial peptides (AMPs) have emerged as a promisingly new group of therapeutic agents for managing infectious diseases and possibly in food preservation. The present study focusses on the isolation of novel bacteriocins from an indigenous sample of cow milk and testing of its antimicrobial activity. Two bacteria isolates (Pediococcus acidilactici and Lactobacillus plantarum) isolated from raw cow milk gotten from Yenegoa, Bayelsa state, Nigeria produced potent bacteriocins on De-Mann Rogosa and Sharpe agar and these were shown to have inhibitory activity against the pathogenic bacteria Escherichia coli. The bacteriocins were heat stable, remaining active at temperatures up to 100°C and functioned well over a wide pH range of 0-10. There was a reduction in activity of the bacteriocins after treatment with proteinase K and peptidase, thus confirming the proteinaceous nature of the compounds. These bacteriocins displayed bacteriostatic and bactericidal activity against E. coli, with a minimum inhibitory concentration of 12.5 μg/ml and minimum bactericidal concentration of 25μg/ml which is lower than that of the conventional antibiotics chloramphenicol (50μg/ml) used as positive control. The bacteriocins produced by lactic acid bacteria isolated from cow milk in this work is effective in inhibiting the growth of E. coli and thus may be of use as a food preservative in the storage of food or as therapeutic agent for the treatment of infections caused by multi-drug resistant E. coli.

Keywords

Bacteriocin Antimicrobial peptide drug resistance

References

  1. Abdelahad B, Koshy P and Sekaran M (2013). Enhanced Production, Purification, Characterization and Mechanism of Action of Salivaricin 9 Antibiotic Produced by Streptococcus salivarius NU10. PLOS ONE. 8(10):1-16
  2. Alakomi, H.L, Skytta, E., Saarela, M, Mattila-Sandholm, T., Latva-Kala, K., and Helander, I.M. (2000). Lactic acid permeabilizes Gram-negative bacteria by disrupting the outer membrane. Appl. Environ. Microbiol. 66:2001–2005.
  3. Cheesbrough, M. (2006). District laboratory practice in tropical countries. Cambridge university press. United Kingdom.
  4. Deegan L.H., Cotter P.D, Colin H., and Ross P., (2006): Bacteriocins: biological tools for bio-preservation and shelf-life extension. International Dairy Journal 16, 1058–1071
  5. Fasoyiro S.B and Adegoke G.O. (2007). Phytochemical characterization and the antimicrobial property of Aframomum danielli extract. Afr. J. Agric. Resr. 2(3):76-79.
  6. Goh, H.F., and Phillip, K. (2015). Purification and characterization of Weissella confusa A3 of dairy origin. PLoS ONE, 10, 1-10.
  7. Harrigan, W.F., 1998. Laboratory Methods in Food Dairy Microbiology. Academic Press, San Diego, CA.
  8. Hati, S., Mandal, S., and Prajapati, J.B. (2013). Novel starters for value added fermented dairy products. Curr. Res. Nutr. Food Sci. 1, 83-91.
  9. Joerger, M. C. and Klaenhammer, T. R. (1986). Characterization and purification of helveticin J and evidence for a chromosomally determined bacteriocin produced by Lactobacillus helveticus 481. J. Bacteriol., 167: 439-446.
  10. Messi, P., Bondi, M., Sabia, C., Battini, R. and Manicardi, G. (2001). Detection and preliminary characterization of a bacteriocin (plantaricin 35d) produced by a Lactobacillus plantarum strain. Int. J. Food Microbiol., 64, 193–8.
  11. Morency, H., Mota-Meira, M., LaPointe, G., Lacroix, C., and Lavoie, M.C. (2001). Comparison of the activity spectra against pathogens of bacterial strains producing a mutacin or a lantibiotic. Can. J. Microbiol. 47, 322-331.
  12. Moreno, I., Lerayer, A.S.L., Baldini, V.L.S. and Leitão, M.F. (2000). Characterization of bacteriocins produced by Lactococcus lactis strains. Braz. J. Microbiol., 31, 184-192.
  13. Nwachukwu MI, Uwaezuoke JC, Nwachukwu IO, Ukaga CN, Anyanwu VE (2009). Phytochemical activities and antimicrobial activities of extracts of calyces of Hibiscus sabdariffa var. altissima on Escherichia coli and Staphylococcus aureus. Nig. J. Microbiol. 23(1):1892-1896.
  14. Ogunbanwo, S.T., Sanni, A.I and Onilude, A.A. (2003). Characterization of bacteriocin produced by Lactobacillus plantarum F1 and Lactobacillus brevis OG1. African Journal of Biotechnology 2:219–227.
  15. Pal, M. (2013): Food spoilage. Ph.D.Lecture Notes. Addis Ababa University, College of Veterinary Medicine, Debre Zeit, Ethiopia.Pp.1-9.
  16. Parada, J.L., Caron, C.R., Medeiros, A.B.P., and Soccol, C.R. (2007). Bacteriocins from lactic acid bacteria: Purification, properties and use as biopreservatives. Braz. Arch. Biol. Technol. 50, 521–542.
  17. Torodov, S. D. and Dicks, L. M. T. (2005). Lactobacillus plantarum isolated from molasses produces bacteriocin active against Gram-negative bacteria. Enzyme and Microbial Technol., 36, 318-326.
  18. Van Geel-Schutten, G.H., Flesch, F., ten Brink, B., Smith, M.R., Dijkhuizen, L. (1998). Screening and characterization of Lactobacillus strains producing large amounts of exopolysaccharides. Appl. Microbiol. Biotechnol. 50, 697–703.
How to Cite

K, A. C., G, I. C., Victor, Z. D., I, O. F., & E, G. E. (2019). Production of Bacteriocins by Lactobacillus plantarum and Pediococcus acidilactici Isolated from Cow Milk. Nigerian Journal of Microbiology, 33(1), 4373 - 4379. https://doi.org/10.67614/njm.2019.pqas4gd3

A. C. K, I. C. G, Z. D. Victor, O. F. I, and G. E. E, "Production of Bacteriocins by Lactobacillus plantarum and Pediococcus acidilactici Isolated from Cow Milk," Nigerian Journal of Microbiology, vol. 33, no. 1, pp. 4373 - 4379, June 2019. doi: 10.67614/njm.2019.pqas4gd3

Share this article:
Facebook X / Twitter LinkedIn
Download PDF View Full Text (PDF) Back to Articles