Abstract

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Fenugreek is an annually globally grown medicinal plant known for its various bioactive components, including antibacterial activities. It have been consumed medically for food as condiments. The proximate analysis of fenugreek seed showed that carbohydrates (37.79 %) had the highest value followed by fibre (22.48%), protein (20.76%), moisture (8.96%), fat (6.57%) and lowest value was recorded for ash (3.44%). The antibacterial activities of fenugreek oil and fenugreek seed extract (ethanol and methanol of 60 g/200 ml) was tested against pathogenic bacteria using open well diffusion and paper disc method at 10 μl, 20 μl, 40 μl, 50 μl and 100 μl concentration. The higher the concentrations, the more significant were the antibacterial activities of both fenugreek oil and seeds extracts. It was observed that the ethanolic extract was the most active against Escherichia coli with the highest zone of inhibition of 14 mm, followed by Klebsiella pneumoniae with 13 mm, while Staphylococcus aureus and Pseudomonas aeruginosa had 12 mm each zone of inhibition. Streptococcus pyogenes had the lowest zone of inhibition of 11 mm. The findings of this study revealed that fenugreek samples extracts were potent against the test bacteria

Keywords: Antibacterial, fenugreek, pathogenic bacteria, proximate composition.