Abstract

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The study was carried out to investigate the denaturing gradient gel electrophoresis (DGGE) for bacteria isolated from petrochemical industrial effluent. Denaturing gradient gel electrophoresis is a molecular technique for separating double stranded PCR products of similar length, but differing sequence composition. Diversity of the isolated and identified microorganisms from the study showed a prevalence of Gram negative bacteria as compared to Gram positive bacteria with Pseudomonas sp. having the highest frequency of isolation (10.33%). The PCR products from GCV6V8F and V6V8R primers on 20%: 80% denaturing gradient had a better DGGE differentiation pattern compared to 30%: 50% DGGE fingerprints of PCR amplified region of GCV6-V8F and V6-V8R of 16S rDNA from effluent bacterial communities

Keywords: Denaturing gradient gel electrophoresis, Polymerase chain reaction, Guanine cytosineV6-V8, Gram negative, 16SrDNA