Abstract

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Nanoparticles has shown to be effective in drug delivery, though, its synthesis are time and energy consuming while, the process is toxic to the environment, hence, the utilization of green synthesis method with plants extracts. The study focused on the green synthesis of nanoparticles and its application on isolates of diseased fish. Four different isolates namely; Bacillus infantis, Klebsiella pneumonae, Escherichia coli, and Vibrio paraheamoliticus were from diseased cat fish (Clarias gariepinus). The silver nanoparticle synthesis was from Psidium guajava L (Guava leaves) and Azadirachta indica (Neem plant leaf) extracts. Phytochemical analysis was carried out on the plants leaf extracts to determine its contents using standard methods. The isolates were obtained through culture method and molecular identification. However, the synthesis of plants extract nanoparticles was characterized using LANMAN spectrophometer, while, antimicrobial activities was carried out using agar well diffusion method and minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were also determined. The influence of the microbial activities of the plants extracts silver nanoparticles synthesized shows the level of impact on the isolated pathogenic organisms. The efficacy was dose dependent, 500 um/mg from guava leaf extract had the highest effect on E. coli while, the least impact was observed on dosage of 62.5 um/mg of E. coli. Neem leaves extract has the highest effect on V. parahaemolyticus at 500 um/mg while, the least was on E. coli at 250 um/mg. The MBC of neem leaf nanoparticle extracts were more potent as it has more clearance level than the guava ‘Therefore, the study revealed that the plant leaf extracts silver nanoparticles synthesized could be used in the treatment of diseases caused by the test organisms

Keywords: Silver nanoparticle, Clarias gariepinus, Minimum inhibitory concentration, Minimum bactericidal concentration