Abstract

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Ulcerative colitis (UC) is an idiopathic inflammatory bowel disease that affects the colonic mucosa and it’s clinically portrayed by diarrhea, abdominal pain and so on. Lactic acid bacteria (LAB) are one of the most significant groups of probiotic organisms, commonly used in fermented dairy products. These group of organisms enhance lactose digestion, stimulate the immune system, prevent and treat diarrhea. In the present study, the therapeutic effects of Lactiplantibacillus plantarum PQ104969 and Lactiplantibacillus plantarum PP893151 on acetic acid-induced ulcerative colitis was evaluated in Wistar albino rats. Acetic acid-induced ulcerative colitis was achieved by intrarectal administration of 5% acetic acid after acclimatization. Wistar rats were then treated orally with either 1 ml of normal saline, L. plantarum PQ104969 (5 × 107 Cfu/ml), L. plantarum PP893151 (5 × 107 Cfu/ml) or prednisolone (2 mg/kg) once a day for 7 days. Disease activity index (DAI) was recorded daily after colitis induction by assessing the symptoms. The rats were sacrificed on day 3 and 7 by cervical dislocation, and colon tissues were isolated for the biochemical analysis of oxidative stress parameters. Depletion of total glutathione (GSH) levels in the colitis group was significantly restored in the L. plantarum PP893151 treated groups, while L. plantarum PQ104969 regulated the expression of proteins, thus alleviated inflammatory response. Both lactic acid bacteria inhibited neutrophil infiltration to suppress myeloperoxidase activity in order to mitigate inflammatory reaction and oxidative stress development in acetic acid induced ulcerative colitis. Hence, Lactiplantibacillus plantarum associated with indigenous fermented foods could be used as an alternative treatment of Ulcerative Colitis

Keywords: Acetic acid, inflammation, ulcerative colitis, Lactiplantibacillus plantarum, oxidative stress