Abstract

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In an era of multidrug resistant bacteria, evaluation of more natural therapeutic options becomes necessary. This study was conducted to determine the antibacterial activity of honey on some clinical bacterial isolates from wound of patients attending Dutse General Hospital. Forty (40) wound swab samples were collected and analyzed out of which 33 demonstrated the growth of organisms. These were identified using morphologic characteristics on selective and differential media, as well as Gram’s and biochemical reaction. Several organisms were isolated including; Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus pyogenes, Escherichia coli, Proteus spp, Klebsiella spp and Pseudomonas spp. S. aureus is the most predominant organism isolated (34%). The antibacterial assay was determined using disk diffusion method with four different concentrations of two honey samples viz: 100% (v/v), 70% (v/v), 50% (v/v) and 30% (v/v). The tested organisms were sensitive to the different concentrations of honey used; however the highest activity was observed in 100% (v/v), the zone of inhibition ranges from 15 19mm in diameter. The highest zone of inhibition was observed in S. aureus (19mm) while Pseudomonas spp had the least (15mm). The antibacterial activity increased with increase in concentration. The MIC of the honey sample 1 and 2 were 1.25v/v for S. aureus, E. coli, Klebsiella spp and 2.5v/v for pseudomonas spp respectively. The MBC of the honey samples (1&2) were 2.5v/v for S. aureus, E. coli, Klebsiella spp and 5.0v/v for Pseudomonas spp respectively. The results of the study revealed that honey has a good antibacterial activity and as such can be used to treat wound infections.

Keywords: Pathogens, Assay, Honey, Isolate. Wound,