Abstract

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he aim of this study was to profile the antimicrobial metabolites synthesized by Streptomycesrimosus-OG95 (KU934251) and to assess the inhibitory activity of the strain against some indicator strains. The production of the antimicrobial compounds was carried out by submerged fermentation. Partial purification of the antimicrobial metabolites was carried out by column chromatography using silica gel while chemical characterization was determined out using FTIR and GC-MS. The Minimum Inhibitory Concentration (MIC) of the partially purified antimicrobial extract was determined by macro broth dilution method. Eleven antimicrobial compounds were identified in the partially purified antimicrobial extract of Streptomyces rimosus-OG95. Bis (2-ethylhexyl) phthalate had the highest abundance at 46.55 %. The MIC ranged between 3.12 to 12.5 mg/L. The antimicrobial compounds produced by Streptomycesrimosus-OG95 possessed antimicrobial activity against Gram positive and Gram negative bacteria.

Keywords: Actinomycetes; antimicrobial metabolites; Broad spectrum activity; Streptomyces rimosus-OG95; Minimum Inhibitory Concentration