Abstract

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Micrococcus spp were among the predominant organisms isolated from earlier developed fermented corn-soybean wastes meal meant for human consumption. Hence the study was aimed at identifying the Micrococcus isolates to their species level. The Micrococcus spp were first subjected to phenotypic analysis and thereafter followed by genotypic analysis using 16SrRNA sequencing method after their DNA isolation and polymerization processes. The phenotypic and genotypic analyses confirmed all the Micrococcus isolates to be Micrococcus luteus Cs which had 99% relatedness to Micrococcus luteus NCTC 2665. The identification of the Micrococcus spp as Micrococcus luteus Cs suggested that the developed meal can be utilized for human consumption since strains of M. luteus are generally regarded as harmless bacteria.

Keywords: Corn-soybean wastes -meal, Identification, Micrococcus luteusCs, Phenotypic, Genotypic