Abstract

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A bacterial strain tolerance to 4-Chlorophenol and Zinc was isolated from sediment of Okrika River that receives effluent from petroleum refinery plant. Out of fifteen (15) bacterial strains isolated, only one of the isolates identified as Bacillus sp. SD12 with highest adhesion to soil (47.4 %) was found to be tolerant to high concentrations of both 4-CP (400 ug/ml) and Zn (200 ug/ml), and hence was selected for further studies. In BH agar medium with mixture of 4-CP and Zn, growth stimulation was observed at 50 ug/ml. The isolate exhibited tolerance to mixtures of 4 CP and other heavy metal ions (Cu, Co and Ni) with growth values of 3.6, 4.7 and ^ 0 cfu/ml for Cu, Co and Ni respectively. Therefore from the results obtained, the organism was found to be good candidate for detoxification of industrial phenolic wastewaters.

Keywords: Sediment Bacteria, Tolerance, 4-Chlorophenol, Zinc, Heavy metals,