Green Synthesis, Characterization and Antimicrobial Effect of Silver Nanoparticles Using Alchornea laxiflora and Spondia mombin Against Microorganisms from Wound and Gastrointestinal Tracts Infections
Abstract
Silver nanoparticles play a huge roles in creating new anti-microbial properties to tackle a quite number of disease-causing microbes. This study aimed at determining the in vitro anti-microbial properties of silver-nanoparticles (AgNPs) green synthesis on multidrug resistance organisms from Nigerian Institute of Medical Research, Lagos State and University college Hospital, Ibadan. The AgNPs was synthesized using extracts of Spondia mombin and Alchornea laxiflora and characterized using UV-vis spectroscopic and fourier-transform infra-red spectroscopy analysis, scanning electron microscopy and Energy-dispersive X-ray analysis. Phytochemical analysis of the ethanolic extract was determined using standard methods. The synthesized AgNPs was assayed for their antimicrobial efficacy using standard methods against some pathogenic organisms. A colour change in the solution from colourless to dark-brown suggested AgNPs formation and spectra of UV-vis at sharp peak of 380–450 nm ascertained the synthesized AgNPs. The fourier-transform spectrum confirmed the presence of some functional groups like hydroxyl group, ammonium ion, aliphatic iodo-compounds and many more which showed major absorption peaks amongst others. The phytochemical screening of ethanolic extracts showed the presence of phenol, saponins, flavonoids, alkaloids among others. The synthesized nanoparticles showed a significant anti-microbial potentials against the tested strains. The minimum inhibitory concentration and minimum bactericidal concentration values ranged between 25 – 50 mg/ml. The medicinal plant extracts used in this study have varied antibacterial properties and ability to mediate the synthesis of AgNPs. The synthesized AgNP suggested their application as a potential therapeutic agents which can be used to formulate new therapeutic drugs for medical use to combat antimicrobial resistance.
Keywords: Alchornea laxiflora-silver nanoparticles; gastrointestinal tract infections; Spondia mombin extracts; green synthesis; wound Infections