Abstract

---

Among the great diversity of etiological agents attributed to urinary tract infections, bacteria are the major causative organisms that are responsible for more than 95% of Urinary Tract Infections ( UTIs). This study aimed at the isolation and identification of bacteria in urine from diabetics and non-diabetics. A total of 300 mid-stream early morning urine specimens were collected from diabetic and non-diabetic patients attending some Hospitals in Rivers State. The specimens were inoculated aseptically onto CHROMagar Orientation plates and incubated aerobically for 24-48 hours at 37oC. Glucose in urine was also measured using uristrip (Combi 9). A total of 95 isolates belonging to 12 genera were isolated. Some of the isolates include Staphylococcus sp., Tatumella sp., Enterobacter sp., Klebsiella sp., Kluyvera sp., Rauotella sp., Proteus sp., Serratia sp., Enterococcus sp., Escherichia sp., Pragia sp., and Photorhabdus asymbiotica with Staphylococcus aureus having the highest occurrence of 19%. Others include Escherichia coli 9%, Kluyvera ascobata 7%, Klebsiella aerogenes 8%, Serratia liquefactens, Enterococcus sp., Pragia fontium constituted 5% each, Enterobacter cloacea , Enterobacter pyrinus, Rauotella planticola, Serratia ficana, Tatumella terrea constituted 4% each, Enterobacter cancergenes, Enterobacter hormaechie constituted 3% each, Klebsiella oxytoca, Proteus mirabilis, Serratia odorifera, Serratia fonticola and Photorhabdus asymbiotica constituted 2% each while Kluyvera intermedia constituted 1% respectively. The study showed different types of bacteria isolated which in most cases were significantly higher in diabetic than non-diabetic patients.

Keywords: Diabetic, Non-Diabetic, Urine, Klebsiella aerogenes, Staphylococcus aureus