Research Article

BACTERIOLOGICAL QUALITY OF PROCESSED, PACKAGED AND STORED CONOPHOR (Tetracarpidium conophorum) NUTS

1 Department of Microbiology, Chukwuemeka Odumegwu Ojukwu University, Uli Campus, PMB 02, Uli, Anambra State, Nigeria
2 Department of Microbiology, Chukwuemeka Odumegwu Ojukwu University Uli Campus, PMB 02, Uli, Anambra State, Nigeria
* Corresponding author: alcamow@yahoo.com
Published: Dec, 2015
Pages: 3022-3028

Abstract

The present study dealt with the formulation and bacteriological quality assessment of two novel methods for processing of conophor (Tetracarpidium conophorum) nuts as food. Cooked-and-roasted (CR) and steeped-and-roasted (SR) conophor nuts stored for 28 days in air-tight transparent glass bottles at room temperature (30±2°C) were analyzed for total aerobic heterotrophic bacterial (TAHB) and total coliform counts. The CR sample recorded an insignificant population of TAHB up to day 7 followed by a viable count (cfu/g) of 3.0 x 103, 5.1 x 103 and 3.3 x 105 for day 14, 21 and 28, respectively. However, an insignificant population of TAHB was observed for the SR sample throughout the study period. There was no coliform growth on the nut samples irrespective of the processing method. Also, regardless of the processing method, the bacterial isolates successively encountered in the samples were identified as Bacillus sp., Corynebactcrium sp., Lactobacillus sp. Staphylococcus aureus and Streptococcus sp. In addition, the SR sample recorded the growth of Micrococcus sp. The most predominant bacteria in both the CR and SR samples were Corynebacterium sp. and Lactobacillus sp. There was persistent decline in moisture content from 6.04% at day 0 to 4.25 % at day 28 for CR sample, and 6.00% at day 0 to 4.26% at day 28 for SR sample. The bacteriological quality of conophor nuts which are processed, packaged and stored as reported herein does not appear to suggest a public health concern.

References

  1. Adebesin A. A., Saromi O. T., Amusa N. A. & Fagade S. O. (2001). Chemical and microbial quality of some groundnut products hawked in Bauchi, a Nigerian city. J. Food Technol. Afr. 6: 53–55.
  2. Ajaiyeoba E. O. & Fadare D. A. (2006). Antimicrobial potential of extracts and fractions of the African walnut (Tetracarpidium conophorum). Afr. J. Biotechnol. 5: 2322-2325.
  3. Alwakeel, S. S. and Nasser, L. A. (2011). Microbial contamination and mycotoxin from nuts in Riyadh, Saudi Arabia. Amer. J. Food Technol. 6(8): 613–630.
  4. Collins C. H., Lyne P. M., Grange J. M. & Falkinnam, J. O. (2004). Collins and Lyne's microbiological methods, 8th Edition. Hodder Arnold Publication, London, 464 pp.
  5. Douglas K. A., Miller, M. J. & Eldridge, L. C. (1970). Almond harvesting, processing and microbial flora. Appl. Environ. Microbiol. 20: 208-214.
  6. Edem C. A., Dosumnu M. I. & Bassey F. I. (2009). Determination of proximate composition, ascorbic acid and heavy metals content of Africa walnut (Tetracarpidium conophorum). Pak. J. Nutr. 8 (3): 225-226.
  7. Enujiugha V. N. (2003). Chemical and functional characteristics of conophor nut. Pak. J. Nutr. 2(6): 335-338.
  8. Enujiugha V. N. & Ayodele-Oni O. (2003). Evaluation of nutrient and antinutrients in lesser known underutilized oil seeds. Intl. J. Food Sci. Technol. 38: 525-528.
  9. Frazier W. C. & Westhoff, D. C. (2000). Food microbiology, 4th edition. Tata McGraw-Hill Publication Inc, New Delhi, 17-34.
  10. Hall H. E. (1971). The significance of Escherichia coli associated with nut meats. Appl. Environ. Microbiol. 25: 230-323.
  11. Holt, J. G. (Ed.) (1982). The shorter Bergey's manual for determinative bacteriology, 8th edition. The Williams and Wilkins Inc., Baltimore, Maryland, U.S.A, pp. 45-50.
  12. Ibeabuchi J. C. (2012). Microbial and physicochemical changes in processed cashew nuts stored in different packaging materials. Intl J. Agric. Rural Dev. 15(2): 1119-1128.
  13. Irtwange, S. V. and Oshodi, A. O. (2009). Shelf-life of roasted cashew nuts as affected by relative humidity, thickness of polythene packaging material and duration of storage. Res. J. Appl. Sci. Engr. Biotechnol. 1 (3): 149-153.
  14. Little, C. L., Jemmott, W., Surman-Lee, S., Hucklesby, L. and de Pinna, E. (2009). Assessment of the microbiological safety of edible roasted nut kernels on retail sale in England, with a focus on Salmonella. J. Food Protect. 72 (4): 853-855.
  15. Little, C. L., Rawal, N., de-Pinna, E. and McLauchlin, J. (2010). Survey of Salmonella contamination of edible nut kernels on retail sale in the U.K. Food Microbiol. 27 (1): 171-174.
  16. Nwabunnia, E. and Otogbor, C. C. (2015). Microflora of the shells of ready-to-eat conophor (Tetracarpidium conophorum) nuts on retail sale in Owerri. Nig. J. Microbiol 29 (1): in press.
  17. Odibo, F. J. C. and Umeh, A. I. (1989). Microbiology of the fermentation of Telfaria seeds for Ogiri production. MIRCEN Journal 5: 217-222.
  18. Oke, O. L. (1995). Leaf protein research in Nigeria, 2nd edition. University of Ibadan Press, Ibadan, Nigeria, pp. 89-94.
  19. Oladapo, A. S., Olufunmilola, A. A., Akintoyese, O. A. and Adepeju, A. B. (2014). Effect of packaging materials on moisture and microbiological quality of roasted cashew nuts (Anacardium occidentale L). Res. J. Eng. Appl. Sci. 3(2): 98-103.
  20. PHLS-ACFDP (2000). Guidelines for the microbiological quality of some ready-to-eat foods sampled at the point of sale. Commun. Dis. Pub. Hlth 3(3): 163-167.
  21. Vural, A. and Erkan, M. E. (2008). The research quality in some edible nut kinds. J. Food Technol. 6(1): 25-28.
  22. Willis, C., Little, C. L., Sagoo, E., de Pinna, E. and Threlfall, J. (2009). Assessment of the microbiological safety of retail dried seeds from retail premises in the United Kingdom with focus on Salmonella spp. Food Microbiol. 26 (8): 847-852.
How to Cite

Nwabunnia, E., & Ezeimo, N. E. (2015). BACTERIOLOGICAL QUALITY OF PROCESSED, PACKAGED AND STORED CONOPHOR (Tetracarpidium conophorum) NUTS. Nigerian Journal of Microbiology, 29(2), 3022-3028.

E. Nwabunnia, and N. E. Ezeimo, "BACTERIOLOGICAL QUALITY OF PROCESSED, PACKAGED AND STORED CONOPHOR (Tetracarpidium conophorum) NUTS," Nigerian Journal of Microbiology, vol. 29, no. 2, pp. 3022-3028, December 2015.

Share this article:
Facebook X / Twitter LinkedIn