Research Article

CULTURE-BASED AND MOLECULAR DETECTION OF THE MIROBIAL QUALITY OF BOREHOLE WATER USED IN SOME HOSTELS IN IHIAGWA, IMO STATE

Ogbulie, T.E.1*, Nwanjo, G.O2, Azuwike, C O.2
1 Department of Microbiology, Federal university of Technology, P.M.B.1526, Ozverri, Nigeria.
2 Department of Biotechnology, School of Science, Federal University of Technology Ovoerri, Nigeria.
* Corresponding author: ogbuliete@nsmjournal.org.ng
Published: Dec, 2015
DOI: 10.67614/njm.2015.1bedcv7p
Pages: 3116-3126

Abstract

Culture-based and molecular detection of the quality of borehole water collected at random from seven hostels in Ihiagwa was undertaken. The bacterial isolates identified using culture-based analysis include Staphylococcus sp, Klebsiella sp, Micrococcus sp, Salmonella sp, Escherichia coli, and Pseudomonas aeruginosa. The presence of fungal isolates such as Trichoderma sp, Aspergillus sp and Rhizopus sp was also observed. Molecular identification of isolates using Polymerase Chain Reaction [PCR assay] with specific primers targeting 16sRNA of coliforms, 16sRNA of total bacteria genera, 16sRNA of Vibrio sp and IVsRNA of total fungi was carried out. Bands were obtained during gel electrophoresis depicting the presence of targeted organisms. The absence of Vibrio sp was evident. This study was carried out to ascertain the level of contamination and non potability of drinking water used within these hostels.

Keywords

Culture-based molecular detection borehole water microorganisms PCR assay primers

References

  1. Agbabiaka, T.O and Sule, I.O (2010). Bacteriological assessment of selected borehole water samples in Ilorin metropolis. International Journal of Applied Biological Research 2(2):31 - 37.
  2. Barrow, G. I and Feltham, R. K. A. (2003). Cowon and Steel's Manual for the Identification of Medical Bacteria 3rd Edition ambridge University Press, United Kingdom 310p
  3. Birmingham, M.E., L.A. Lea, N. Ndayiminje, S. Nkurikiye, B.S. Hersh, J.G. Wells and M.S. Ijeming (1997). Epidemic Cholera in Burundi, Patterns of transmission in the Gadat Rift Valley Lake Region. Lancet 349: 981 –983.
  4. Cheesbrough, M. (1994). Medical Laboratory Manual for Tropical Counties. Vol. II. Microbiology University Press, Cambridge. Pg. 58 – 273.
  5. Chukwurah, E.I. (2001) Aquatic Microbiology. Otoba Press Limited, Onitsha, Nigeria.
  6. Fawole, O.O., Lateef, A. and Amaefuna. M (2002). Microbiological examination of drinking water in Ogbomosho metropolis, South West Nigeria. Science Focus,1 (1) : 16 – 20.
  7. Holt JG (1984) The Shorter Berger's Manual of Determinative Bacteriology, Williwms Wilkiins, Baltimore. 356p
  8. Hugenholtz, P., Goebel, B.M. and Pace, N.P. (1998): Impact of Culture-Independent studies on the emerging phylogenetic view of bacterial diversity. J. Bacterial, 180: 4765 –4774.
  9. Ibe, S.N. and Okplenye, J.I. (2005): Bacteriological Analysis of Borehole Water in Uli, Nigeria. African Journal of Applied Zoology and Environmental Biology. 7: 116 – 119.
  10. OECD WHO, (2003). Assessing Microbial Safety for Drinking Water, Improving Approaches and Methods. Organization for Economic Co-Operation Development IWA Publishing Company Pg. 291.
  11. Ogbulie, T. E and Akujiobi, T. C (2006). Bacteriological Qualities of Bottled Water sold in Imo State, Nigeria. International Journal of Natural and Applied Science. 2(2): 122-128.
  12. Ogbulie, T. E., Akujiobi T.C. and Kalu, I. G (2007). Assessment of the Physicochemical Quality of Commercial bottled water sold in Imo State, Nigeria. International Journal of Natural and Applied Science 3(1): 131-137.
  13. Ogbulie, T. E. (2008). Quality assessment of some sachet water sold in Owerri Imo State, Nigeria. International Journal of Tropical Agriculture Food Systems 2(1): 34-38.
  14. Ogbulie, J.N., Duru, C.N., Awujo, N.C. and Ogbulie, T.E. (2009). Physicochemical Quality of Municipal Borehole Water in Imo State, Nigeria. Pakistan Journal of Medical Research 48(1): 1-5.
  15. Okpako, E.C, Osuagwu, A., N., Duke A., E and Ntui, V., O (2009): Prevalence and significance of fungi in sachet and borehole drinking water in Calabar, Nigeria. African Journal of Microbiology Research 3(2): 056 – 061.
  16. Okpokwasili, G.C and T.C. Akujobi (1996) Bacteriological indicators of tropical water quality. Environment Toxicol.Water Quality. 11: 77 – 82.
  17. USEPA (2001) Current Drinking Water Standard. United States Environmental Protection Agency. Washington DC.
  18. USFDA (2015). Factors that influence microbial growth In: Evaluation and definition of potentially hazardous foods. United States Food and Drug Administration. Safe Practices for Food Processes http://www.fda.gov/Food/FoodScienceResearch/SafePracticesforFoodProcesses/ucm094145.htm
  19. Ward, J.V., Malard, F. Tockner, K. and Vehklinger, U. (1998). Influence of ground water on surface water condition in a glacial flood plain of Swiss Alps. Water, Air and Soil Pollution 101 (1 – 4): 15 – 24.
  20. WHO (2008). Guidelines for Drinking Water Quality 3rd Edition Vol. 1. Incorporating 1st and 2nd addenda WHO Press Geneva 668 pp.
How to Cite

T.E., O., G.O, N., & O., A. C. (2015). CULTURE-BASED AND MOLECULAR DETECTION OF THE MIROBIAL QUALITY OF BOREHOLE WATER USED IN SOME HOSTELS IN IHIAGWA, IMO STATE. Nigerian Journal of Microbiology, 29(2), 3116-3126. https://doi.org/10.67614/njm.2015.1bedcv7p

O. T.E., N. G.O, and A. C. O., "CULTURE-BASED AND MOLECULAR DETECTION OF THE MIROBIAL QUALITY OF BOREHOLE WATER USED IN SOME HOSTELS IN IHIAGWA, IMO STATE," Nigerian Journal of Microbiology, vol. 29, no. 2, pp. 3116-3126, December 2015. doi: 10.67614/njm.2015.1bedcv7p

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