Research Article

Detection of Second Line Drug Resistant Mycobacterium tuberculosis among Patients Attending National Tuberculosis and Leprosy Training Center Zaria, Nigeria

Suleman, M1*, Ado, A. S2, Aliyu, M.S3
1 Department of Microbiology, Ahmadu Bello University, Zaria
2 Department of Microbiology, Faculty of Science Kano University, of Science and Technology, Wudil.
3 National Tuberculosis and Leprosy Training Center, Zaria.
* Corresponding author: mikhasulemada@yahoo.com
Published: Jun, 2020
DOI: 10.67614/njm.2020.xrhkz4z0
Pages: 4987-4997

Abstract

Tuberculosis (TB) is an infectious disease cause by Mycobacterium tuberculosis and it remain one of the major public health problem. This study was to detect resistance to second line anti-tuberculosis among patient attending national tuberculosis and leprosy training centre Zaria, Nigeria using Lowenstein Jensen proportion (phenotypic) methods. A total 6125 patients were recruited, out of which 775 (12.6%) were MTB positive and 100 out of 775 were resistant to rifampicin by Xpert MTB/RIF with a prevalence of 13%. Out of 100, (90%) were culture positive while 7 (7%) were culture negative and 3 (3%) were contaminated. All of the ninety (90) samples that were culture positive were confirmed as Mycobacterium tuberculosis complex using immunochromatoghapic test. Seventy (77.7%) isolates were found to be pan susceptible while twelve (13.3%) and eight (9%) were resistant to Fluoroquinolones and Aminoglycoside respectively. Resistance of Mycobacterium tuberculosis to second line anti-TB drugs in this study was observed to be high among age groups 31-45 and 16-30 years who are male living in urban setting. It was also observed to be high among non-reactive HIV that have not taken alcohol before and among those that were not previously treated with TB drugs, even though there’s no statically association between the drug resistance and social-demographic or risk factors in this study. This study has shown high prevalence of drug resistant tuberculosis among patient attending the National Tuberculosis and Leprosy Training Centre, Zaria, Nigeria. The proportions of resistance detected in this study serve as possible indicator of the future emergence of XDR-TB in Nigeria. There is a need for close monitoring of TB patients for proper treatment and compliance to prevent drug resistant tuberculosis. However for the correct management patients with resistance to any of the SLD, results must be confirmed by phenotypic drug susceptibility testing.

Keywords

Tuberculosis (TB) Immunochromatoghapic National Tuberculosis and Leprosy Training Centre

References

  1. Akerele, O. (1993). Nature's medicinal bounty: don't throw it away. World Health Forum. 14: 390-395.
  2. Archibong Etim, Igboeli J, Chinechendo N., Okoro Nnenna C., and Obika Ifeanyi, (2017). Microbiological Assessment of Some Liquid Herbal Medications Sold in Awka Metropolis, Anambra State. Bioengineering and Bioscience, 5(3): 37-46.
  3. Braide W., Oranusi S. U., Nwaoguikpe R.N., Offor-Emenike I.U., Nwosu I.I., Akobondu C. I., Chike-Reginald C. and Popgbara L.B. (2013). Evaluation of the microbiological status and antibacterial susceptibility pattern of some herbal remedies administered orally in Nigeria. Research Journal in Engineering and Applied Sciences 2(1):35-42.
  4. Cheesbrough M. (2000) Diaric Laboratory Practice in Tropical Countries Part 2: Cambridge University Press, Cambridge;1:70.
  5. Coon, J.T., Emest, E and Parax, G. (2002) "A systematic review of adverse effectand drug interaction." Drug safety;5: 323- 344.
  6. Esimone, CO, Chah, KF, and Ikejide SC (2001). Microbiological quality of herbal preparations marketed in South East Nigeria. Journal of Natural Remedies, 2(1): 42-48.
  7. Idu, M., Erhabor, J. O. and Idele, S. O. (2011). Microbial Load of Some Medicinal Plants Sold in Local Markets of Benin City, Nigeria. International Journal of Medical and Aromatic Plants. 1(3):272-277.
  8. Igbeneghu O.A and Adebayo L. (2016). Assessment of the microbial quality of some oral liquid herbal medicines marketed in Ile-Ife, South-western Nigeria. African Journal of Microbiology Research, 10(38): 1618-1624.
  9. Kunle OF, Egharevba HO, and Ahmado PO (2012). Standardization of herbal medicines – A review. International Journal of Biodiversity and Conservation 4: 101 – 112.
  10. Mendes, E., Herdeiro, M. T and Pimentel, F. (2010). The Use of Herbal Medicine Therapies by Cancer Patients. Acta Medica Portuguese. 23(5):901-908.
  11. Nester, E., W., Roberts, C.E., Pearsall, M T., Anderson, D.G. and Nester, M.T (1998). Microbiology. A Human Perspective, 2nd Edition WBC/McGraws Hills New York, USA, pp. 434 -435.
  12. Odedara O.O. and Memuletiwon E.J., (2014). microbial quality of some locally consumed herbal concoctions in Abeokuta metropolis, Nigeria. Journal of Natural Science, Engineering and Technology, 13:58-66.
  13. Oladosu O. Peters, Umar Y., A, Salawuden A, Izebe K, Adamu M T and Aboh M (2018). Antibacterial Activity of Soaps Indigenously Made inGombe Metropolis, Nigeria. Journal of Natural Remedies, DOI: 10.18311/jnr/2018/22434.
  14. Pelczar, MJ (Jr), Chan, E.C.S and Krieg, N.R 1993). Microbiology: Concepts and Applications, 1st edn. McGraw-Hill Inc., New York, USA. pp. 80-100, 158-161, 370.
  15. Prescott, L.M., Harley, J.P and Klein, D.A (1999). Isolation of Pure Bacterial Culture from Specimen: Microbiology International, 4th Edition: BostonWCB McGraw's Hill Companies: pp: 714 – 796.
  16. Rajapandiyan, K., Shanthi, S. and Vidya, S. (2013). Assessment of Microbial Quality in Marketed Herbal Drugs Sold in Trichy City. International Journal of Pharmaceutical, Chemical and Biological Sciences. 3(3):894-898.
  17. Selvamohan T and Sandhya T (2012). Studies on bactericidal activity of different soaps against bacterial strains. Journal of Microbiology and Biotechnology Research 5:646-650
  18. Sofowora, E.A (1994). Medical Plant and Traditional Medicine in Africa. University of Ife Press, Nigeria: pp: 1 – 23.
  19. Underwood E. (1992) Pharmaceutical Microbiology (Hugo WB, Russel AD, ed.) Blackwell Scientific Publication, 5th edn, Oxford 353-368
  20. United States Pharmacopeia (2009). A harmonized standard for sterility test. 10th June, ReV.1.Cor 3.
  21. United States Pharmacopeia USP (2018). Microbiological Tests. In: The National formulary 36, US Pharmacopeial Convention, Inc. Vol.4, 2929 – 6017.
  22. World Health Organization (1998). Regulatory situation of herbal medicine: A worldwide review. World Health Organization, Geneva.
  23. World Health Organization (2000). General Guidelines on Research and Methodologies for Evaluation of Traditional Medicine.
  24. World Health Organization. Dept. Of Technical Cooperation for Essential Drugs and Traditional Medicine. (2007). Guidelines for assessing quality of herbal medicines with reference to contaminants and residues. Geneva: World Health Organization. P 105.
  25. Wylie, J. L and Norwick, D. L. (2005). Molecular Epidemiology of Community and Health Care- Associated Methicillin Resistant Staphylococcus aureus in Manitoba, Canada. Journal of Clinical Microbiology. 43(6):2830-2836.
How to Cite

M, S., S, A. A., & M.S, A. (2020). Detection of Second Line Drug Resistant Mycobacterium tuberculosis among Patients Attending National Tuberculosis and Leprosy Training Center Zaria, Nigeria. Nigerian Journal of Microbiology, 34(1), 4987-4997. https://doi.org/10.67614/njm.2020.xrhkz4z0

S. M, A. A. S, and A. M.S, "Detection of Second Line Drug Resistant Mycobacterium tuberculosis among Patients Attending National Tuberculosis and Leprosy Training Center Zaria, Nigeria," Nigerian Journal of Microbiology, vol. 34, no. 1, pp. 4987-4997, June 2020. doi: 10.67614/njm.2020.xrhkz4z0

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