Research Article

Determination of Phytochemical and Antimicrobial Activities of Corn Starch Extract on Escherichia coli and Salmonella typhi

Erhimu Loveday Obakpororo1*, Tasie Floretta1, Nwachukwu Ujunwa Felicia1, Obodo Chidubem Nathaniel1
1 Enugu State University of Science and Technology, Faculty of Applied Natural Sciences, Department of Applied Microbiology and Brewing, P.M.B 01660, Agbani, Enugu, Nigeria.
* Corresponding author: nwachukwujiawa77@yahoo.com
Published: Dec, 2019
Pages: 4660-4667

Abstract

The phytochemical and antimicrobial effect of cornstarch extracts was investigated. Corn starch of yellow and white corn variety were extracted successively with ethanol, methanol and distilled water. These crude extracts were assessed for antimicrobial activities against Escherichiacoli and Salmonella typhi. Escherichia coli were sensitive to the methanolic and ethanolic extracts of white corn starch with zones of inhibition of 22mm and 20mm respectively. Escherichia coli was also sensitive to ethanolic extract of yellow corn starch with a zone of inhibition of 22mm. Salmonella typhi was also sensitive to ethanolic and methanolic extracts of white maize starch with zones of inhibition of 20mm and 21mm respectively. The minimum inhibitory concentration of extracts of various corn varieties on E. coli and Salmonella typhi were investigated.The minimum inhibitory concentration of ethanolic (1.56mg/ml) and methanolic extract (0.78mg/ml) of white maize starch had zones of 17mm and 19mm on Escherichia coli respectively. The minimum inhibitory concentration of ethanol and methanol extract of white corn starch on Salmonella typhi where found to possess zones of inhibition17mm and 18mm respectively, while the minimum inhibitory concentration of ethanolic extract (0.78mg/ml) of yellow corn starch on Escherichiacoli had zones of inhibition of 18mm. Phytochemical screening of both varieties of cornstarch revealed the presence of alkaloid, tannin, saponins and terpenoids. Sensitivity testing of the phytochemicals present revealed that tannins had zone of inhibition on the test organisms (Salmonellatyphi and Escherichia coli (23.20mm and 25mm) respectively, while the other phytochemicals had no zones of inhibition.

Keywords

Antimicrobial Corn starch Escherichia coli Phytochemical Salmonella typhi

References

  1. Chopra, S., Cocedolone, S. M., Bushman, S., Sangar, V., McMullen, M.D. and Peterson, T. (2003). The maize unstable factor for orange 1 is a dominant epigenetic, modifier of a tissue specifically silent allele of pericarp color 1. Genetics, 163(3): 1135-1146.http://www.genetics.org/content/163/3/1135
  2. Falana, M. B., Bankole, M. O., Omemu, A. M. and Oyewole, O. B. (2012). Antimicrobial potentials of some selected microorganisms associated with supernatant solution of fermented maize masa conditon from two varieties of maize grains. African Journal of Microbiological Research, 6: 4095-4101. https://doi.org/10.5897/AJMR11.055
  3. Fernandez, A., Torres - Gener, S. and Lagaron, J. M. (2009). Novel route of stabilization of bioactive anti-oxidants by encapsulation in electrospun fibers of zein prolamine. Food Hydrocolloids, 23; 1427-1432.http://scholar.google.com/citations?user=n0w9ZP1MAAAAJ&hl=en
  4. Hagiwara, A., Miyashita, K., Naskanishi, T., Sano, M., Tamano, S., Kadota, J and Shirai, T. (2001). Pronounced inhibition by a natural anthocyanin, purple corn colour, of 2-amino - 1 - methyl-6-phenyl limidazo (4,5-b) pyridine (Plip) - associated colorectal cuciogenesis in male F 344 rats (retreated with 2,2- dimethylhydrazine, Cancer Letters, 171(2):1-25.https://www.ncbi.nlm.nih.gov/pubmed/1148 5824
  5. Higgins, J. A. (2004). Resistant starch: Metabolic effects and potential health benefits. Journal of AOAC International, 87(3): 761-768.https://www.ncbi.nlm.nih.gov/pubmed/152 87677
  6. Himi, E., Mares, D. J., Yanagisawa, A. and Noda, K. (2002). Effect of grain color gene on grain dormancy and sensitivity of the embryo to abscisic acid (ABA) in wheat. Journal of Experimental Botany, 53 (374): 1569-1574.https://doi.org/10.1093/jxb/erf005.
  7. Humphrey, T. (2004). Salmonella, Stress responses and food safety. Nature Reviews Microbiology, 2: 504-509.doi:10.1038/nrmicro907.
  8. Kim, W. K., Chong, M.K., Kang, N.E., Kim, M.H and Park, O.J. (2003). Effect of resistant starch from corn or rice on glucose control, colonic events, and blood lipid concentrations in streptozotocin-induced diabetic rats. The Journal of Nutritional Biochemistry, 14(3): 166-172.https://www.ncbi.nlm.nih.gov/pubmed/1274 2453
  9. Kopsell, D. A., Armel, G. R., Mueller, T. C., Sams, C. C., Deyton, D. E., McElroy, J. S. and Kopsell, D. E. (2009). Increase in nutritionally important sweet corn kernel carotenoids follow mesotrione and atrazine applications. Journal of Agricultural and Food Chemistry, 57:6362-6368. DOI: 10.1021/JP9013313.
  10. Kumar, D. and Jhariya, N.A. (2013). Nutritional Medicinal and Economical Importance of Corn: A mini-review. Research Journal of Pharmaceutical Sciences, 2:7-8.www.isca.in
  11. Omemu, A. M., Oyewole, O.B. and Bankole, M. O. (2007). Significance of yeast in the fermentation of maize for Ogi production. International Journal of Food Microbiology, 24: 571-516. DOI:10.1016/j.fm.2007.01.006
  12. Ostlund Jr. R.E. Racette, S.B. Okeke, A and Stenson, W. F. (2002). Phytosterols that are naturally present in commercial corn oil significantly reduce cholesterol absorption in humans. American Journal of Chemical Nutrition, 75: 1000 - 1004. https://doi.org/10.1093/ajcn/75.6.1000.
  13. Song, X., Yi, L., Baoyun, X., Jamie, H., Jeanne, M. R., Hong, J. Chumnei, Z., Ross, J. M., Richard, D. and David, F. S. (2011). Shotgun glycomics: a microarray strategy for functional glycomics. Nature Methods, 8:85-90.doi: 10.1038/nmeth.1540
  14. Teniolas, O. D. and Odunfa, S. A. (2001). The effects of processing methods on the level of Lysine, Methionine and the general acceptability of Ogi processed using starter cultures. International Journal of Food Microbiology, 63: 19.https://www.infona.pl/resource/bwmeta1.element.elsevier-377fed35-3101-3af3-9564-11009
  15. Wang, X., Brown, I.L, Khaled, D., Mahoney, M. C., Evans, A. J. and Conway, P.L. (2002). Manipulation of Colonic bacteria and volatile fatty acid production by dietary high amylose maize (amylomaize) starch granules. Journal of Applied Microbiology, 93: 390-397.http://onlinelibrary.wiley.com/doi/10.1046/j.1365-2672.2002.01704.x
  16. Williams, R. (2000). Antimicrobial Resistance a global threat. Essential Drug Monitor, 28: 1-2. http://apps.who.int/medicinedocs/pdf/s2248e/s2248.pdf
  17. Zhao, Z., Egashira, Y. and Sanedes, H. (2005). Phenolic antioxidants richly contained in corn bran are slightly bio-available in rats. Journal of Agricultural and Food Chemistry, 53(12): 5030-5035. DOI:10.1021/j0501109
How to Cite

Obakpororo, E. L., Floretta, T., Felicia, N. U., & Nathaniel, O. C. (2019). Determination of Phytochemical and Antimicrobial Activities of Corn Starch Extract on Escherichia coli and Salmonella typhi. Nigerian Journal of Microbiology, 33(2), 4660-4667.

E. L. Obakpororo, T. Floretta, N. U. Felicia, and O. C. Nathaniel, "Determination of Phytochemical and Antimicrobial Activities of Corn Starch Extract on Escherichia coli and Salmonella typhi," Nigerian Journal of Microbiology, vol. 33, no. 2, pp. 4660-4667, December 2019.

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