Research Article

EFFECT OF STORAGE CONTAINERS ON THE BACTERIOLOGICALQUALITY OF WATER FROM DIFFERENT SOURCES

1 Department of Microbiology, Federal University Of Technology Oiuerri Imo State
2 Department of Microbiology, University Of Port Harcourt, Rivers State
* Corresponding author: malachyosuji760@gmail.com
Published: Dec, 2015
Pages: 3148-3158

Abstract

This research work was therefore carried out to assess the effect of different containers on the quality of different water samples and the effect of long storage time on the bacterial load (bioload) of the water samples. Spread plate count technique was adopted using Nutrient, Salmonella/Shigella, MacConkey and Thiosulphite Citrate bile sucrose agar. Calabash, Glass, Metal and Plastic containers were used in the storage of rainwater, river water and tap water. In rain water stored in calabash, the total heterotrophic bioload decreased from 9.21ogcfu/ml to 5.31ogcfu/ml. For the river water stored in glass container, the bioload decreased from 7.01ogcfu/ml to 5.01ogcfu/ml. In other containers, there were such decreases. Several factors were noticed to be responsible for the bioload changes. The factors include; the toxins and acids produced as secondary metabolites during the stationary phase, temperature variation, nutrient depletion, and pH. Plastic container recorded the highest bioload decrease. People are advised to use plastic containers for water storage and they should filter the water before using so as to remove the biofilms formed at the wall of the containers due to death of bacteria when toxins are produced and nutrient depleted.

References

  1. Berge C.C; Allen MJ and Smith B.B (1991). National field Evaluation Of a defined substrate method for the simultaneous Enumeration of total Coliform and E.coli from Water: Comparison with the standard multiple tube Fermentation method:Collaborative method. Journal of Association of Official Analytical Chemistry 74:526-529
  2. Clean Water Act, Section 303(d), 33 U.S.C. § 1313; Section 305(b), 33 U.S.C. § 1315(b); Section 314, 33 U.S.C § 1324
  3. Diersing, Nancy (May 2009). "Water Quality: Frequently Asked Questions". PDA. NOAA. http://floridakeys.noaa.gov/pdfs/wqfaq.pdf. Retrieved 2009-08-24.
  4. Hodgson K, Manus L. A drinking water quality framework for South Africa. Water SA. 2006;32(5):673-678 [1].
  5. Geo,R.G;Janet,S.B and Morse K.I(2001)Enteric Gram Negative Rods. In: Medical microbiology. 21st edition.Lange Medical book/Me Graw- hill Pp225-228
  6. Harvey H.W. (1992) on changes taking place in seawater during storage. Journal of marine Biology association United Kingdom 25:225
  7. Melvin, D.J,David OJ;John,T.N and James,L.W( 1991)World of Chemistry. Sanders college publisher London pp22
  8. Nester, E. W; Anderson, D.G;Robert,C.E; Pearsall, N. N and Nester M.T (2004) Bacterial Disease of the Upper Alimentary system: In Microbiology, a Human perspective. 4th edition. McGraw-Hill Comp. New York;pp 616-617
  9. Oghulie J. N; Uwaezuoke, J. C and Ogiehqr,S.I (1998) Water Quality Microbiology In Introductory Microbiology practical. Springfield Publisher Nig.pp 114-115.
  10. Okafor, N (1985) Aquatic and waste microbiology. 1st Edition. Fourth Dimension Publisher Enugu.pp 1-3,107-126.
  11. Taylor C.B. and Collins V.G. (2004) Development of Bacteria in water stored in Glass containers. Freshwater biological association. Way castle Ambleside Westmorland.
  12. United States Environmental Protection Agency (EPA). Washington, DC. "Water Quality Standards Review and Revision."2006.
  13. U.S. EPA. Washington, DC."Methods for Measuring the Acute Toxicity of Effluents and Receiving Waters to Freshwater and Marine Organisms." Document No. EPA-821-R-02-012. October 2002.
How to Cite

M.J., O., & N., O. N. (2015). EFFECT OF STORAGE CONTAINERS ON THE BACTERIOLOGICALQUALITY OF WATER FROM DIFFERENT SOURCES. Nigerian Journal of Microbiology, 29(2), 3148-3158. https://doi.org/10.67614/njm.2015.4074hhq2

O. M.J., and O. N. N., "EFFECT OF STORAGE CONTAINERS ON THE BACTERIOLOGICALQUALITY OF WATER FROM DIFFERENT SOURCES," Nigerian Journal of Microbiology, vol. 29, no. 2, pp. 3148-3158, December 2015. doi: 10.67614/njm.2015.4074hhq2

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