Research Article

Identification Profile of Micrococcus luteus Cs Associated with Fermented Corn-Soybean Wastes-Meal

1 Enugu State University of Science and Technology, Faculty of Applied Natural Sciences, Department of Applied Microbiology and Brewing, P.M.B 01660, Agbani, Enugu, Nigeria.
* Corresponding author: nwachukwuujunwafelicia@nsmjournal.org.ng
Published: Dec, 2019
Pages: 4747-4752

Abstract

Micrococcus spp were among the predominant organisms isolated from earlier developed fermented corn-soybean wastes meal meant for human consumption. Hence the study was aimed at identifying the Micrococcus isolates to their species level. The Micrococcus spp were first subjected to phenotypic analysis and thereafter followed by genotypic analysis using 16SrRNA sequencing method after their DNA isolation and polymerization processes. The phenotypic and genotypic analyses confirmed all the Micrococcus isolates to be Micrococcus luteus Cs which had 99% relatedness to Micrococcus luteus NCTC 2665. The identification of the Micrococcus spp as Micrococcus luteus Cs suggested that the developed meal can be utilized for human consumption since strains of M. luteus are generally regarded as harmless bacteria.

References

  1. Akbar, A., Sitara, U., Imran, A., Muhammad, N. and Shabir, A. K. (2014). Isolation and characterization of biotechnology potent Micrococcus luteus strain from environment. Pakistan Journal of Zoology, 46 (4):967-973.
  2. Fazlani, S., Abubaker, M., Shah, S., Ul Hassan,M. and Arshed, M.(2008). Bio-morphological characteristics of bacterial species identified from mastitic milk samples of camel. The Internet Journalof Veterinary Medicine, 6: 1.
  3. Fox, X., Fox, A., Elssner, T., Feigley, C. and Salzberg, D. (2010).MALDI TOF Mass spectometry speciation of Staphylococci and their discrimination from Micrococci isolated from indoor air of school rooms. Journal of Environmental Monitoring, 12: 917-923.Doi:10.1039/B925250A
  4. Ganz, T., Gabayan, V., Liao, H., Liu, L., Oren, A., Graf, T. and Cole, A. (2002). Increased inflammation in lysozyme M-deficient mice in response to Micrococcus luteus and its peptidoglycan. Blood,101(6):
  5. George-Okafor,U., Nwachukwu,U. and Mike-Anosike, E. (2018). Screening and identification of predominant Lactobacillus spp from fermented milled-mixed corn-soybean wastes. International Journal of Scientific and Engineering Research, 9(2):2050-2060. http://www.ijser.org.
  6. Greenblatt, C.L., Baum, J., Klein, B.Y., Nachtson, S., Koltunov, V. and Cano, R.J. (2004). Micrococcus luteus – Survival in Amber. Microbial Ecology, 48 (1): 120-127.DOI:10.1007/s00248-003-2016-5
  7. Haga, S.,Hirano, Y., Murayama, O., Miller, B.C., Moore, J.E. andMatsuda,M. (2003).Structural analysis and genetic variation of the 16S–23S rDNAinternal spacer region fromMicrococcus luteusstrains.Letters in Applied Microbiology, 37: 314-317. doi:10.1046/j.1472-765X.2003.01399.x
  8. Liu, H., Xu, X., Ma, Y. and Zhou, P.(2000). Characterization of Micrococcus antarcticus sp.nova, a psychrophilic bacterium from Antarctica. International Journal of Systematic and Evolutionary Microbiology, 50: 715-719.DOI:10.1099/00207713-50-2-715
  9. Mohagnia, O. Nagpal, R., Kumar, M. Bhardwaj, A., Yadav, M., Jain, S., Marotta, F., Singh, V., Parkash, O. and Yadav, H. (2008). Molecular approaches for identification and characterization of Lactic acid bacteria. Journal of Digestive Disease, 9:190-198.https://doi.org/10.1111/j.1751-2980.2008.00345.x
  10. Kocur, M., Kloos, W.E. and Schleifer, K.H. (2006). The Genus Micrococcus. In: Dworkin, M., Falkow, S., Rosenberg, E., Schleifer, K.H. and Stackebrandt, E., Eds., The Prokaryotes, Springer,New York,961-971.http://dx.doi.org/10.1007/0-387-30743-5_37
  11. Pawar, Y., Surekha, P., Sarath, M.K., Pradeep, S. and Sailas, B. (2016). Micrococcus luteus strain BAA2, A Novel isolate produces carotenoid pigment. Electronic Journal of Biology,12:1.ejbio.imedpub.com/micrococcus-luteus-strain-baa2-a-novel-isolate-produces-caroteno
  12. Pereira, J. H, Goh, E., Keasling, J. D., Beller, H. R. and Adams, P. D. (2011). Structure of FabH and factors affecting the distribution of branched fatty acids in Micrococcus luteus. Acta Crystallographica Section D, Biological Crystallography, 68(10):1320-1328. doi: 10.1107/S0907444912028351
  13. Young, M., Artsatnov, V., Beller, H. R., Chandra, G., Chater, K. F., Dover, L. G. and Greenblatt, C. L. (2009). Genome sequence of the fleming strain of Micrococcus luteus, a simple free-living Actinobacterium. Journal of Bacteriology, 192(3): 841-860. doi:10.1128/JB.01254-09.
  14. Zhang, Z., Schwart, S., Wagner, L. and Miller, W. (2000). A greedy algorithm for aligning DNA sequences. Journal of Computer Biology, 7: 203-214.DOI:10.1089/106652700050081478
How to Cite

Felicia, N. U., Ogechi, G. U., & Ada, O. D. (2019). Identification Profile of Micrococcus luteus Cs Associated with Fermented Corn-Soybean Wastes-Meal. Nigerian Journal of Microbiology, 33(2), 4747-4752.

N. U. Felicia, G. U. Ogechi, and O. D. Ada, "Identification Profile of Micrococcus luteus Cs Associated with Fermented Corn-Soybean Wastes-Meal," Nigerian Journal of Microbiology, vol. 33, no. 2, pp. 4747-4752, December 2019.

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