Research Article

In Vitro DIGESTIBILITY EVALUATION OF RICE BRAN MEAL INCORPORATED WITH PRECIPITATE p-D MANNANASE FROM Penicillium italicum

Ekundayo, T. C1*, Olaniyi, O.O1
1 Department of Microbiology, Federal University of Technology, P.M.B 704, Akure, Nigeria
* Corresponding author: cyruseusthem@gmail.com
Published: Aug, 2015
DOI: 10.67614/njm.2015.c86g15f2
Pages: 2760-2766

Abstract

The effect of addition of crude and ammonium sulphate ((NH^SQi) precipitation of P-D-mannanase produced by Penicillium italicum on in vitro dry matter digestibility (IVDMD) and in vitro protein digestibility (IVPD) of rice bran meal intended for animal feed formulation was studied. Firstly, the crude enzyme (non-precipitated), and (NH-1)SO4 precipitated enzyme were incorporated in the series of enzymes activities. Secondly, the effect of p-D-mannanase preparation and incubation time was evaluated on IVDMD and IVPD. Crude enzyme produced in basal medium supplemented with rice bran meal as the carbon source was assayed by dinitrosalicylic acid method and precipitated from culture supernatant with 70% ammonium sulphate concentration. Measurement of IVDMD and IVPD were carried out using pepsin pancreatine assay. Results from statistical analysis showed significant interaction between the kind of enzyme preparations and activities on IVPD and IVDMD. In vitro protein digestibility (IVPD) of rice bran meal prepared with different enzyme preparations increased significantly (P

Keywords

Penicillium italicum; in vitro dry matter digestibility; in vitro protein digestibility; rice bran meal; p-D-Mannanase

References

  1. Agosin, E., Monties, B. and Odier, E. (2006). Structural changes in wheat straw components during decay by lignin-degrading white rot fungi in relation to improvement of digestibility for ruminants. Journal Sciences and Food Agriculture, 36(10): 925-935.
  2. Akinyele, B. J. and Agbro, O. (2008). Increasing the nutritional values of plantain wastes by the activities of fungi using the solid state fermentation technique. Research Journal of Microbiology, 2(2):117-124.
  3. Akinyele, B. J., Olaniyi, O. O. and Adetunji, C. O. (2013). Screening and optimization of nutritional conditions for mannanase production by Penicillium italicum LAD-A5 in solid state cultivation. E3 Journal of Biotechnology and Pharmaceutical Research, 4(2): 35-41.
  4. Aroupin, D. J. and Olaniyi, O. O. (2013). Screening and Identification of Mannanase-Producing Fungi Isolated from Selected Agricultural Wastes. British Microbiological Research Journal (In press).
  5. El-Naggar, M. Y., El-Aassar, S. A., Youssef, A. S., El-Sersy, N. A. and Beltagy, F. A. (2006). Extracellular β-mannanase production by the immobilization of the locally isolated Aspergillus niger. International Journal of Agricultural Biology, 8: 57-62.
  6. Gélinas, P. and Barrette J. (2007). Protein enrichment of potato processing waste through yeast fermentation. Bioresource Technology, 98(5): 1138-1143.
  7. Ghaly, A. E., Kamal, M. and Correia, L. R. (2005). Kinetic modelling of continuous submerged fermentation of cheese whey for single cell protein production. Bioresource Technology, 96(10): 1143-1152.
  8. Iqbal, M. N. T., Muhajumnad, A., Ishtiaq, A. and Shahbaz, H. (2010). Media optimization for hyper-production of carboxymethylcellulase using proximally analysed agro-industrial residues with Trichoderma harzianum under SSF. International Journal of Agricultural Veterinary Medicine and Sciences, 4(2): 47-55.
  9. Jackson, M. E., Fodge, D. W. and Hsiao, H. Y. (1999). Effects of β-mannanase in corn-soybean meal diets on laying hen performance. Poultry Science, 78: 1737-1741.
  10. Ke, L., Wu, Q. and Zhang, D. (2011). Bioconversion of rape straw into a nutritionally enriched substrate by Ganoderma lucidum and yeast. African Journal of Biotechnology, 10(29): 5648-5653.
  11. Khanongnuch, C., Sa-nguansook, C., Lumyong, S. (2006). Nutritive quality of β-mannanase treated copra meal in broiler diets and effectiveness on some fecal bacteria. International Journal of Poultry Science, 5(11): 1087-1091.
  12. Lowry, O. H., Rosebrough, J. N., Farr, L. A. and Randall J. R. (1951). Protein measurement with the Folin-Phenol reagents. Journal of Biological Chemistry, 193: 265-275.
  13. Maronmani, H. K. and Sreekantiah, K. R. (1987). Saccharification of sugar cane baggasse with enzymes from Aspergillus ustus and Trichoderma viride. Enzyme Microbiology and Technology, 9: 484-488.
  14. Miller, L. (1959). Use of dinitrosalicylic acid reagent for determination of reducing sugar. Analytical Chemistry, 31: 208-218.
  15. Mukherjee, R. and Nandi, B. (2004). Improvement of in vitro digestibility through biological treatment of water hyacinth biomass by two Pleurotus species. International journal of Biodeterioration and Biodegradation, 53(1): 7-12.
  16. Parani, K. and Eyini, M. (2010). Effect of cofungal treatment on biodegradation of coffee pulp waste in solid state fermentation. Asian Journal of Experimental Biological Sciences, 1(2): 352-359.
  17. Pitt, J. I. and Hocking, A. D. (1997). Fungi and food spoilage, 2th Edition, Blacki Academic, London, U.K. Pp. 414.
  18. Purwadaria, T. and Haryati, T. (2003). In Vitro digestibility evaluation of coconut meal incorporated with precipitate β-D-mannanase from Eupenicillium javanicum. Journal of Microbiology Indonesia, 8(1): 19-21.
  19. Purwadaria, T., Haryati, T., Darina, J. and Munazat, O. I. 1995 (). In Vitro digestibility evaluation of coconut meal using Aspergillus niger NRRL 337. Rul. Peternaan Special ed. Pp. 375-382.
  20. Ray, S., Pubols, M. H. and McGinnis, J. (1981). The effect of purified guar degrading enzyme on chick growth. Poultry Sciences; 61: 488-494.
  21. Stabnikova, O., Wang, J. Y., Ding, J. H. and Tay, J. H. (2005). Biotransformation of vegetable and fruit processing wastes with yeast biomass enriched with selenium. Bioresource Technology, 96(6): 747-751.
  22. Villas-Bôas, S. G., Esposito, E. and Mendonça, M. M. (2003). Bioconversion of apple pomace into a nutritionally enriched substrate by Candida utilis and Pleurotus ostreatus. World Journal of Microbiology and Biotechnology, 19(5): 461-467.
  23. Zhang, R., Li, X. and Fadel, J. (2002). Oyster mushroom cultivation with rice and wheat straw. Bioresource Technology, 82(3): 277-284.
How to Cite

C, E. T., & O.O, O. (2015). In Vitro DIGESTIBILITY EVALUATION OF RICE BRAN MEAL INCORPORATED WITH PRECIPITATE p-D MANNANASE FROM Penicillium italicum. Nigerian Journal of Microbiology, 28(1), 2760-2766. https://doi.org/10.67614/njm.2015.c86g15f2

E. T. C, and O. O.O, "In Vitro DIGESTIBILITY EVALUATION OF RICE BRAN MEAL INCORPORATED WITH PRECIPITATE p-D MANNANASE FROM Penicillium italicum," Nigerian Journal of Microbiology, vol. 28, no. 1, pp. 2760-2766, August 2015. doi: 10.67614/njm.2015.c86g15f2

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