Research Article

Isolation and Growth Trend of Selected Strains of Pseudomonas in Petrochemical Contaminated Soil

Ayogu, C.V1*, Ifeanyi, V.O.2, Obasi, N. P.1
1 Aquatic Bioresources Training Centre, Adiabo, National Biotechnology Development Agency, Nigeria.
2 Department of Microbiology, Michael Okpara University of Agriculture Umudike, Abia State.
* Corresponding author: ayogucv@nsmjournal.org.ng
Published: Dec, 2020
DOI: 10.67614/njm.2020.mxd6jvnl
Pages: 5313-5324

Abstract

Some toxic chemicals from petroleum products constitute environmental pollutants which are considered threats to ecological system. This study was performed to monitor the survival growth of some selected indigenous Pseudomonas strains isolated from petrochemical contaminated soil. Standard microbiological procedures and 16S rRNA technique were employed during isolation and identification of the strains. The physiochemical characteristics of the soil sample were conducted using standard laboratory procedure. Optimization of culture conditions were carried out under varying pH concentrations, moisture content, temperature and nutrients (N:P). The identified strains were P. aeruginosa PAER4 119, P. putida B6-2 and P. mendocina NK-01. The result of the soil physiochemical properties showed particle size distribution of 70.60 % for sand, 21.40 % for loam and 8.00 % for clay. The recorded pH value of 5.8, moisture content of 12.5 % and total petroleum hydrocarbons of 7.4 % were observed. The results of the optimization showed highest cell growth at temperature of 30 oC, pH value of 7, moisture content of 20 %, and nitrogen phosphorus ratio of 10:1. Maximum growth recorded during bioremediation by the strains were 2.90 x 1010 cfu/g for P. putida B6-2, 2.86 x 1010 cfu/g for P. aeruginosa PAER4 119 and 2.84 x 1010 cfu/g for P. mendocina NK-01. Cell growth increased with time. This study revealed the ability of Pseudomonas strains to grow and survive in the petrochemical contaminated soil. Hence, this study can be employed for successful bioremediation studies.

Keywords

Petrochemicals contaminated soil Pseudomonas strains.

References

  1. Adeyemi, MM, Adebote, D.A., Amupitan, J.O. and Oyewale, A.O. (2010). Antifeedant activity of Quercetin isolated from the stem bark of Bobgunnia madagascariensis (Desv.) J.H.Kirkbr and Wiersema. (Caesalpiniaceae). Australian J. of Basic and Appl. Sci, 4(8): 3342-3346.
  2. Alexander, M. (2000). Aging bioavailability and overestimation of risk from environmental pollutants. Environ. Sci. and Technol, 34(20): 4259-4265.
  3. American Public Health Association (APHA) (2004). Standard methods for the examination of waste water. 21st Edition, American Public Health Association, Washington.
  4. Atagana, H.I, Haynes, R.J and Wallis, F.M. (2003). Optimization of soil physical and chemical conditions for the bioremediation of creosote-contaminated soil. Biodeg, 14: 297-307.
  5. Bharathi, S. and Vasudevan, N. (2001). Utilization of hydrocarbon by Pseudomonas fluorescens isolated from a petroleum contaminated soil. Environ. Int, 26: 413-416.
  6. Brenner, J., Kreig, R. and Stanley, T. (2005). Bergey's Manual of Systematic Bacteriology. The Probacteria, Part A. Introductory Essay, Springer, New York, pp 27.
  7. Darine, T. and Ridha, M. (2013). Microbial Inoculants and Their Impact on Soil Microbial Communities: A Review. Micro. Diversity for Biotech, 1-11.
  8. Garrity, G.M, Brenner, D.J., Krieg, N.R and Staley, J.T. (2005). Bergey's Manual of Systematic Bacteriology (second ed.), Springer-Verlag, New York, 323-359.
  9. Hamzah, A., Rabu, A., Farzarul, R. and Yussoff, N.A. (2010). Isolation and Characterization of Bacteria Degrading Sumandak and South Angsi Oils. Sains Malaysiana, 39(2): 161-168.
  10. Holt, J.G., Krieg, N.G., Sneath, P.H.A., Staley, J.T. and Williams, S.T. (1994). Bergey's Manual of Determinative Bacteriology, 9th edn. Baltimore, MD: Williams and Williams.
  11. Holt, J.G., Krieg, N.R., Sneath, P.H.A., Staley, J.T. and Williams, S.T. (1998). Bergey's Manual of Determinative Bacteriology. Baltimore, M.D., USA: 9th Edition, Williams and Wilkins.
  12. Kalme, S., Ghodake, G. and Gowindwar, S. (2007). Red HE7B degradation using desulfonation by Pseudomonas desmolyticum NCIM 2112. Int Biodeter Biodegr, 60: 327-333.
  13. Karamalidis, A.K., Evangelou, A.C., Karabika, E., Koukkou, A.I., Drainas, C. and Vondras, E.A. (2010). Laboratory scale bioremediation of petroleum contaminated soil by indigenous microorganisms and added Pseudomonas aeruginosa strain Spet". Biores. Technol, 101(16): 6545-52.
  14. Khashayar, T. and Mahasa, T. (2010). Biodegradation potential of petroleum hydrocarbons by microbial diversity in soil. World appl. sci. jol, 8(6): 750-755.
  15. Kilbane, J., Ranganathan, R., Cleveland, L., Kayser, K.J., Ribiero, C. and Linhares, M.M. (2000). Selective Removal of Nitrogen from Quinoline and Petroleum by Pseudomonas ayucida IGTN 9M. Appl. Environ. Microbiol, 66: 688-693.
  16. Kottler, B.D. and Alexander, M. (2001). Relationship of properties of Polycyclic aromatic hydrocarbons to sequestration in soil. Environ.Poll. 113: 293-298.
  17. Lloyd, A.C. and Cackette, T.A. (2001). Diesel engines: environmental impact and control. J. Air Waste Manag. Assoc, 51(6): 809-847.
  18. Larroche, C., Ricke, S.C., Dussap, C.G., Gnansounou, E. (eds). Biofuels: Alternative Feedstocks and Conversion Processes, pp. 101-122. Academic Press, Elsevier Science, San Diego.
  19. Lee, M., Kim, M.K., Singleton, I., Goodfellow, M. and Lee, S.T. (2006). J. Appl. Microbiol, 100: 325-333.
  20. Lee, S., Speight, J.G. and Loyalka, S.K. (2014). Ethanol from corn. In: Handbook of Alternative Fuel Technologies. CRC Press, Taylor & Francis Group, Boca Raton, FL.
  21. Leys, N.M., Bastiaens, L., Verstraete, W. and Springael, D. (2005). Influence of the carbon/nitrogen/phosphorus ratio on polycyclic aromatic hydrocarbon degradation by Mycobacterium and Sphingomonas in soil. Appl. Microbiol. and Biotechnol, 66(6): 726-36.
  22. Liu, S., Wang, C., Zhang, S., Liang, J., Chen, F and Zhao, K. (2012). Formation and distribution of polycyclic aromatic hydrocarbons (PAHs) derived from coal seam combustion: A case study of the Ulanqab lignite from Inner Mongolia, Northern China. Int. J. of Coal Geol. 90: 126-134.
  23. Luo, Q., Zhang, I-G., Shen, X-R, Sui, X, Fan, Z-Q. (2013). Characterization of a novel diesel oil-degrading Pseudomonas sp. Strain F4. Fresenius Environ Bullet, 22: 689-697.
  24. Maduemo, L., Coppotelli, B.M., Alvarez, H.M. and Morelli, I.S. (2011). Isolation and characterization of indigenous soil bacteria for PAH bioaugmentation of contaminated soil of semiarid Patagonia, Argentina. Intern. Biodeterior. and Biodeg, 65(2): 345-346.
  25. Mahalingam, P.U. and Nithya, S. (2014). Optimization of growth condition for diesel oil degrading bacterial strains. Advan. in Appl. Sci. Resear, 5(6): 91-96.
  26. Mishra, S. and Jyot, J., et al., (2001a). In situ bioremediation potential of an oily sludge degrading bacterial consortium. Current Microbiol, 43: 328-329.
  27. McPherson, M.J. and Muller, S.G. (2000). PCR-The basic from background to bench, 1st ed.; Bios Scientific Publishers, Oxford, New York.
  28. Oberhofer, T.R., Rowen, J.W and Cunningham, G.F. (1999). Characterization and identification of gram-negative, nonfermentative bacteria. J. Clin. Microbiol, 5: 208-209.
  29. Prescott, M., Lansing, Harley, P., John, Klein, A. and Donald, (2005). Microbiology, sixth edition, McGraw-Hill Higher Education.
  30. Potin, O., Veignie, E and Rafin, C. (2004). Biodegradation of polycyclic aromatic hydrocarbons (PAHs) contaminated soil, FEMS Microbiol. Eco, 51-71.
  31. Public Health England, (2015). Identification of Pseudomonas species and other non-Glucose Fermenters. Bacteriology-Identification. Microbiol. Serv. Stand. Unit, 3: 1-41.
  32. Raieta, K., Muccillo L., Colantuoni, V. (2015). A novel reliable method of DNA extraction from olive oil suitable for molecular traceability. Food Chem, 172: 596.
  33. Rajasekar, A., Babu, T.G., Pandian, S.T., Maruthamuthu, S., Palaniswamy, N. and Rajendran, A. (2007). J. Ind. Microbiol. Biotechnol, 34: 589-598.
  34. Razavi, D.S. and Lakzian, A. (2007). Evaluation of chemical and biological consequences of soil sterilization methods. Caspian J. of Environ. Sci, 5: 87-91.
  35. Renner, R. (1999). EPA to strengthen persistent, bioaccumulative and toxic pollutant controls—mercury to be targeted. Environ Sci Technol, 33: 62-63.
  36. Rodriguez-Martinez, E.M. (2006). Microbial diversity and bioremediation of a hydrocarbon contaminated aquifer. Intern. J of environ. Resea. and pub. Health, 3(3): 290-300.
  37. Sathishkumar, M., Binupriya, A.R., Baik, S.H. and Yun, S.E. (2008). Biodegradation of Crude Oil by Individual Bacterial Strains and a Mixed Bacterial Consortium Isolated from Hydrocarbon Contaminated Areas. Clean, 36: 92-96.
  38. Shallu, S., Hardik, P. and Jarodi, D.P. (2014). Factors Affecting the Rate of Biodegradation of Polyaromatic Hydrocarbons. Inu. J. Pure App. Biosci, 2(3):182-192.
  39. Shkidchenko, A.N., Kobzev, E.N., Petrikevich, S.B., Chugunov, V.F. and Boronin, A.M. (2004). Biodegradation of black oil by microflora of the Bay of Biscay and biopreparations. Process Biochem, 39: 167-1-169.
  40. Shukor, M.Y., Dahalan, F.A., Jusoh, A.Z., Muse, R., Shamaan, N.A., and Syed, M.A., (2009). J. Environ. Biol, 30(1): 145-150.
  41. Soccol, C.R., Faraco, V., Karp, S., Vandenberghe, L.P.S., Thomaz-Soccol, V., Woiciechowski, A. and Pandey, A. (2011). Lignocellulosic bioethanol: current status and future perspectives. In: Pandey, A.,
  42. Tamura, K., Peterson, D., Peterson, N., Stecher, G., Nei, M. and Kumar, S. (2011). MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Molec. Biol. and Evol, 28(10): 2731.
  43. Vidali, M. (2001). Bioremediation: An overview. Pure Appl. Chem, 73(7): 1163-1172.
  44. Yergeau, E., Sanschagrin, S., Beaumier, D. and Greer, C.W. (2012). Metagenomic Analysis of the Bioremediation of Diesel-Contaminated Canadian High Arctic Soils. PLoS ONE 7(1)e30058.
  45. Zouaoui, B. and Bouziane, A. (2012). Production, optimization and characterization of the lipase from Pseudomonas aeruginosa .Rom. Biotech. Lett, 17(2): 7187-93.
How to Cite

C.V, A., V.O., I., & P., O. N. (2020). Isolation and Growth Trend of Selected Strains of Pseudomonas in Petrochemical Contaminated Soil. Nigerian Journal of Microbiology, 34(2), 5313-5324. https://doi.org/10.67614/njm.2020.mxd6jvnl

A. C.V, I. V.O., and O. N. P., "Isolation and Growth Trend of Selected Strains of Pseudomonas in Petrochemical Contaminated Soil," Nigerian Journal of Microbiology, vol. 34, no. 2, pp. 5313-5324, December 2020. doi: 10.67614/njm.2020.mxd6jvnl

Share this article:
Facebook X / Twitter LinkedIn
Download PDF View Full Text (PDF) Back to Articles