Research Article

Microbial Assessment and Antibiogram of Bacteria Isolated from Air samples around Dumpsites in Igando, Lagos, Nigeria

Fashola, M. O.1*, Grillo, J. A1, Obayori, O. S1, Opere, B. O.1, Eguakun, E. A.1
1 Department of Microbiology, Faculty of Science, Lagos State University, Ojo, Lagos Nigeria.
* Corresponding author: muibat.fashola@lasu.edu.ng
Published: Jun, 2020
DOI: 10.67614/njm.2020.17vwx93f
Pages: 4829-4841

Abstract

This study assessed the level of microbial contamination of air around three municipal dumpsites in Igando and determined the antibiogram of the bacterial isolates. Total aerobic bacteria count (TABC) and total enterobacterial count (TEC) of air around the dumpsites nearby amusement park and gas station were measured using the “sedimentation method. The microbial isolates were identified using phenotypic and biochemical characteristics and their antibiotic sensitivity was determined using Kirby Bauer disk diffusion. Total aerobic bacteria count (TABC) at the three dumpsites ranged between 1.9 x 107 and 2.6 x107 cfu/m3 while the amusement park and gas station recorded 2.2 x 107 and 2.4 x 107 cfu/m3 respectively. The TECs of air at the dumpsites varied between 6.6 x 106 and 7.9 x 106 cfu/m3, while the amusement park and gas station recorded 6.4 x 106 and 2.9 x 106 cfu/m3 respectively. Staphylococcus sp. had the highest percentage occurrence of 47 followed by Bacillus 17. Among fungi, Rhizopus sp. had the highest percentage occurrence 26.67 followed by Aspergillus 26.47. For the Gram-positive bacteria, Bacillus spp. recorded the highest percentage resistance (HPR) of 40 against tetracycline. Twenty percent of the Gram positive bacterial isolates were resistant to tetracycline and erythromycin respectively. Among the Gram negative bacterial isolates, 33.33% were resistant to cefuroxime and 22.22% were resistant to gentamicin and ofloxacin respectively. The high level of microbial contamination of the air at and around the dumpsites constitute potential risk to health

Keywords

Air quality Antibiogram Dumpsites Igando Microbial quality

References

  1. Agbor, R., Ekpo, I. Ekalwo, U., Okigbo, A., and Kalu, S. (2013). Groundwater quality assessment of some selected boreholes in Calabar. World Rural Observers, 5(3):37-41.
  2. Akoteyon, I. S Mbata, U. A. and Olalude, G.A. (2010). Investigation of Heavy Metal Contamination in Groundwater Around Landfill Site in a Typical Sub-Urban Settlement in Alimosho, Lagos State. Journal of Applied Sciences in Environmental Sanitation, 6(2):155-163
  3. Amuda, O. S., Adebisi, S. A., Jimoda, L. A. and Alade, A. O. (2014). Challenges and Possible Panacea to the Municipal Solid Wastes Management in Nigeria. Journal of Sustainable Development Studies, 6(1): 64 -70.
  4. Angaye, Tariwari C.N. Daokoru-Olukole, Chidinma and Abowei, Jasper F.M. (2018). Microbiological Assessment of Municipal Solid Waste Dumpsites in Yenagoa Metropolis, Bayelsa State, Nigeria. Biotechnological Research, 4(1); 24-33.
  5. Barrow M. R. and Feltham R.K.A. (1995). Cowan and steels manual for identification of Medical bacteria, 3rd edition. Cambridge University, Cambridge.330 p
  6. Breza-Boruta, B. (2012). Bioaerosols of the municipal waste landfill site as a source of Microbiological air pollution and health hazard. Ecological Chemistry and Engineering, 19 (8): 851-862.
  7. Burge, HA. (2001). Fungi toxic killers or allergy nuisances? Annals of Allergy Asthma and Immunology, 87(3): 52-56
  8. Cadena, M., Durso, L. M., Miller, D. N., Waldrip, H. M., Castleberry, B. L., Drijber, R. A and Wortmann, C. (2018). Tetracycline and sulfonamide antibiotic resistance genes in soils from Nebraska organic farming operations. Frontiers in Microbiology, 9 (1283): 1– 10.
  9. Cheesebrough. M. (2006). District Laboratory Practice in Tropical Countries, part 2, second edition Cambridge University press, The Edinburgh Building, Cambridge, United Kingdom, 38, 62-69.
  10. CLSI (2005). Performance standards for antimicrobial susceptibility testing; fifteenth informational supplement, Clinical and Laboratory Standard Institute Wayne, Pa. M100-S15, vol.25, no.1.
  11. Dada, E.O. and Aruwa, C.E. (2014). Microorganisms associated with urine contaminated soils around lecture theatres in Federal University of Technology, Akure. Nigeria. Inernational Journal of Applied Biotechnology and Biotechnology Research. 2: 79-85
  12. Després, V. R., Huffman, J., A., Burrows, S.M., Hoose, C., Safatov A.S., Buryak, G., Frohlich-Nowoisky, J. Elbert, W., Andreae, M. and Pöschl, U. (2012). Primary biological aerosol particles in the atmosphere: a review. Tellus B: Chemical and Physical Meteorology. 64(1):155-168.
  13. Douwes, J. (2003). Bioaerosol health effects and exposure assessment: Progress and prospects. Annals of Occupational Hygiene., 47(3): 87-200
  14. Ekpo, I. Agbor, R., Albert, P., Okpako, E. and Inyang, P. (2013). Assessment of heavy metal status and bacteria population in municipal boreholes water in landfill and non-landfill areas in Calabar Municipality, Cross-River State, Nigeria. International Journal of Engineering and Applied Science, 24(3):31-41.
  15. Eni, D., Ubi, I. Arikpo, E., and Digha, N. (2014). Vulnerability assessment of boreholes located close to lemman landfill in Calabar metropolis, Nigeria. International Journal of Physical and Human Geography, 2(2):6-15
  16. Ezenwa, Y. (2014). Geochemistry and petrogenesis of mafic-ultramafic rocks of Northwest Obudu Plateau, Southeastern Nigeria: major element evidence. Global Journal of Pure and Applied Science, 20:37-44.
  17. Fraczek, K. and Ropek, D. (2011). Municipal waste dumps as the microbiological threat to the natural environment. Ecological Chemical and Engineering, 18 (1): 93-110.
  18. Golofit-Szymczak, M. and Górny, Rafał L. (2010) Bacterial and fungal aerosols in air-conditioned office buildings in Warsaw, Poland—The winter season International Journal of Occupational Safety and Ergonomics. 16(4): 465-476
  19. Guevart, E., Noeke, J. Sole, J.M. Essomba and M. Edienguele et al., (2006).Factors contributing to endemic cholera in Douala, Cameroon. Medical. Tropical. (Mars), 66: 283-291.
  20. Heo, Y., Park, J., Lim, S.I., Hur, H.G., Kim, D. and Park, K., (2010). Size-resolved culturable airborne bacteria sampled in rice field, sanitary landfill, and waste incineration sites. Journal of Environmental moniroring. 12 (8): 1619-1624.
  21. Huang, C.H., Lee, C.H., Li, F., Ma, Y.and Su, H., (2002). Characterisation of bioaerosols in municipal landfill sites. Atmospheric Environment. 36(4): 438-442.
  22. Igborgbor, J.C. and Ogu, G.I. (2015). Microbial assessment of air in the vicinity of some dump yards in Delta State. International Organization of Scientific Research. Journal of Engineering, 05 (01): 07-15.
  23. James, G. C. and Natalie, S. (2001). Microbiology. A laboratory Manual (ed.). Pp. 211-223
  24. Kalwasińska, A., Burkowska, A. and Swiontek Brzezinska, M., (2014). Exposure of workers of municipal landfill site to bacterial and fungal aerosol. CLEAN-Soil, Air, Water, 42(42: 1337-1343
  25. Kazmierczuk, M. and Bojanowicz-Bablok, A., (2014). Bioaerosol concentration in the air surrounding municipal solid waste landfill. Environmental Protection and Natural Resources. 25(2): 17-25.
  26. Kim, K.H, Jahan, S.A. and Kabir, E. (2013). A review on human health perspective of air pollution with respect to allergies and asthma. Environment Inernational, 59: 41-52.
  27. Kummer, V., and Thiel, W.R., (2008). Bioaerosols: sources and control measures. International Journal of Hygiene and Environmental Health 211:299-307.
  28. Lagos State Waste Management Authority (LAWMA), (2010). Report. Statistical Analysis of Landfill Report by Various Agencies.
  29. Lawnickei-Walczyk, A., Gorny, R. L., Glofit-Szymczak, M., Niesler, A and Wlazlo, A. (2013). Occupational exposure to airborne microorganisms, endotoxins and beta-glucans in poultry houses at different stages of the production cycle. Annals of Agricultural and Environmental Medicine. 20.
  30. Le Goff, O., Godon, J.J., Bachelery, H., Steyer, J., Wery, N. (2012). A new combination of microbial indicators for monitoring composting bioaerosols. Atmospheric Environment, 61: 428-43
  31. Li, Y.P., Yang, L.W., Meng, Q.L., Qiu, X.H., Feng, Y. (2013). Emission characteristics of airborne microbials in a municipal sewage treatment plant in Xian, China. Aerosol Air Quality Research. 13: 343-349
  32. Madhukar R. and Srikantaswamy S. (2013). Assessment of Air Quality in Bidadi Industrial Area, Ramanagaram District, Karnataka, India. Journal of Environmental Science, Computer, Science Engineering and Technology. 2(4): 1135-1140.
  33. Mansour, F.A., El-Dohlob, S.M., Abdel Hameed, A.A., Kamel, M.M., El-Gendy, S.A. (2012). Microorganisms in the air over a bio-solid waste landfill in Egypt. The Journal of America, 851-862.
  34. Marchand, G., Lavoie, J., Lazure, L. (2012). Evaluation of bioaerosols in a municipal solid waste recycling and composting plant. Journal of Air and waste Management Association, 45: 778-781.
  35. Mbata, T. (2007). Prevalence and antibiogram of urinary tract infections among prison inmates in Nigeria. Inernet Journal of Microbiology, 3(2): 8 – 16.
  36. Mitra, S., Mukherjee, S., Naha, S., Chattopadhyay, P., Dutta, S. and Basu, S. (2019). Evaluation of co-transfer of plasmid –mediated fluoroquinolones resistance genes and blaNDM gene in Enterobacteriaceae causing neonatal septicaemia. Antimicrobial Resistance and Infection Conurol, 8946: 2 -15.
  37. Mwaikono, K. S., Maina, S and Gwakisa, P. (2015). Prevalence and antimicrobial resistance phenotype of enteric bacteria from a municipal dumpsite. Journal of Applied and Microbiology, 3(3): 82 – 94.
  38. Mwanze, P.I., Nwaru, L. M., Oranusi, S., Dimkpa, U., Ukwu, M. U., Babatunde, B. B., Anake, A. T., Jatto, W. and Asagwara, C. E (2007). Urinary tract infection in Okada village. Prevalence and antimicrobial susceptibility pattern. Scientific Research and Essays, 2(4): 112 – 116.
  39. National Population Commission 2006 NPC (2006). Federal Republic of Nigeria Official Gazette 96(2).
  40. Nwaka, G. I. (2005). The urban informal sector in Nigeria: towards economic development, environmental health and social harmony.Global Urban Development Magazine 1(1): 1-11
  41. Nyandjou, Y. M. C., Yakubu, S. E., Abdullahi, I. O. and Machido, D. A. (2017). Multidrug resistant Escherichia coli 0157:H7 from refuse dumpsites. Nigerian Journal of Scientific Research, 16(3).
  42. Obasi, N., Akubugwo, E., Ugbogu, O. and Otuchristian, G. (2012). Assessment of physicochemical properties and heavy metal bioavailability in dumpsite along Enugu- Port Harcourt express ways, Southeastern, Nigeria. Asian Journal of Applied Sciences, 5(6):342-356.
  43. Obire, O., Nwabueta, O. and Adule, S. (2002). Microbial community of a waste dumpsite. Journal of Applied Science and Environmental Management, 6(1):78-83.
  44. Odeyemi, A. T. (2012). Antibiogram status of bacterial isolates from air around dump sites of Ekiti State Destitute Centre at Ilokun, Ado – Ekiti, Nigeria. Journal of Microbiology Research, 2(2): 12 – 18.
  45. O'Gorman, C.M. and Fuller, H.T., (2008). Prevalence of culturable airborne spores of selected allergenic and pathogenic fungi in outdoor air. Atmospheric. Environment, 4: 4355-4368.
  46. Oliveira, D., Borges, A. and Simões, M. (2018). Staphylococcus aureus toxins and their molecular activity in infectious diseases. Toxins, 10 (6): 252-256
  47. Oyedele, O.A. and Oyedele, A.O (2017). Impacts of Waste Dumps on the Health of Neighbours: A Case Study of Olusosun Waste Dumpsite, Ojota, Lagos State Nigeria. Journal of Civil, Consruction and Environmental Engineering, 2(1): 27-33
  48. Panthi, V., Chand and Shrestha, U. (2008). Microbial Air Pollution ; A case study of Sodisol sanitary landfill. Asian Journal of Applied. Science, 1(1): 94-97.
  49. Roodbari, A., Naddafi, K. and Javid, A., (2013). Measurements of bioaerosols in the air around the facilities of waste collection and disposal. Environmental Protection and Engineering, 39 (4): 105-109.
  50. Stanley, C. N., Ezealisiji, K. M. and Chukwuemeka-Eze, N. (2018). Antimicrobial susceptibility of soil bacteria isolated from rusting metal solid waste dump site, municipal waste dump site and mechanic/engine oil contaminated soil samples in Port Harcourt. Inernational Journal of Current Advanced Research, 7(2K): 10343 – 10347.
  51. Ugboma C. J., Ndubuisi-Nnaji U. U. and Ugwu, C. C. and Ugwuanyi, O. W. (2015). Prevalence and diversity of generic extended spectrum β-lactam-resistant enterobacteria in healthy pigs. Notulae Sciemia Biologicae, 7(3): 272 – 280.
  52. Vilavert, L., Nadal, M., Figueras, M. J. and Domingo, J. L. (2012). Volatile organic compounds and bioaerosols in the vicinity of a municipal waste organic fraction treatment plant. Human exposure risks. Environmental Science and Pollution. Research. Inernational, 19: 96-104.
  53. William G. L., Brett J. G., Francoise M. B., Stephen B.M., Brandon F. L, Paul A. J. and Millie P. S. (2017). Sampling and Characterization of bioaerosols. National Institute for Occupational Safety and Health (NIOSH) Manual of Analytical Methods (NMAM) 5th Edition Pp 1-115.
  54. Williams J. O. and Hakam K. (2016). Microorganisms associated with dump sites in Port Harcourt Metropolis, Nigeria Journal of Ecology and the Natural Environmen, 8 (2): 9-12.
  55. Yassin, M.F. and Almouqatea, S. (2010). Assessment of airborne bacteria and fungi in an indoor and outdoor environment. International Journal of Environmental Science and Technology, 7(3): 535-544,
  56. Zucker, B.A. and Muller, W., (2004). Airborne endotoxins and airborne Gram-negative bacteria in a residential neighborhood. Waer Air Soil Pollution, 158: 67-75.
How to Cite

O., F. M., A, G. J., S, O. O., O., O. B., & A., E. E. (2020). Microbial Assessment and Antibiogram of Bacteria Isolated from Air samples around Dumpsites in Igando, Lagos, Nigeria. Nigerian Journal of Microbiology, 34(1), 4829-4841. https://doi.org/10.67614/njm.2020.17vwx93f

F. M. O., G. J. A, O. O. S, O. B. O., and E. E. A., "Microbial Assessment and Antibiogram of Bacteria Isolated from Air samples around Dumpsites in Igando, Lagos, Nigeria," Nigerian Journal of Microbiology, vol. 34, no. 1, pp. 4829-4841, June 2020. doi: 10.67614/njm.2020.17vwx93f

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