Microbial Assessment of Herbal Products in Ota And Its Environs
1 Department of Biological Sciences, College of Natural and Applied Sciences, Bells University of Technology, P. M. B. 1015, Ota, Ogun State, Nigeria
* Corresponding author: foalao@bellsuniversity.edu.ng
* Corresponding author: foalao@bellsuniversity.edu.ng
Abstract
This study was carried out to assess the microbial quality of herbal medicinal products being sold in Ota and its environs. A total of 24 bacterial isolates were identified from 32 herbal products purchased from 8 sellers in different parts of Ota. The total plate count (TPC) for bacteria in the herbal samples analysed ranged from 1.1 x 10 to 9.3 x 1OCFU/ml; while the total fungal count ranged from 1.2 x 10 to 9.8 x lOCFU/ml. The bacterial and fungal counts of the herbal products increased with the period of storage (24, 48, 96 hours). Bacteria! isolates obtained from this study belong to four genera; Escherichia coli, Vibrio cholerae, Salmonella typhi and Staphylococcus aureus. Sixty percent of the herbal products analysed were contaminated with Escherichia coli, 50% by Vibrio cholerae, 30% of Staphylococcus aureus, and 40% with Salmonella typhi. The antibiotic resistance and susceptibility patterns of the isolates showed 45% and 36% of Escherichia coli were resistant to ofloxacin and ciprofloxacin respectively; all the Staphylococcus aureus were susceptible to ofloxacin and ciprofloxacin; 80% of the Vibrio cholerae were resistant to ofloxacin; and 50% of the Salmonella typhi isolates were resistant to ofloxacin and ciprofloxacin. All the bacterial isolates recovered from herbal products in this study were resistant to cefuroxime, ampicillin, and ceftazidime. The result of this study revealed the need for adequate quality control measure to be put in place for herbal preparations used for commercial purpose in order to safeguard the health of the public.
Keywords
Microbial
herbal products
isolates
storage
Ota.
References
- Abba, D., Inabo, H.I., Yakubu, S.E. and Olonitola, O.S. (2009). Contamination of herbal medicinal products marketed in Kaduna Metropolis with selected pathogenic bacteria.
- African Journal of Traditional, Complementary and Alternative Medicine.6: 70-77.
- Adewunmi, C.O. and Ojewole, J.A.O (2004). Safety of traditional medicines, complementary and alternative medicines in Africa, African Journal of Traditional, Complementary and Alternative Medicines. 1: 1-3.
- Akerele, O. (1988). Medicinal plants - and primary healthcare needs: an agenda for action. Fitoterapia. 59:355-363.
- CDC. (2002). Coliform bacteria and drinking water. Centers for Disease Control and Prevention. http://www.bfnd.wa.gov/2002.
- Izzo, A. A. and Ernst, E. (2009). Interactions between herbal medicines and prescribed drugs: an updated systematic review.Drugs. 69(13): 1777-1795.
- Lowy, F. D. (1998). Staphylococcus aureusinfections. TheNew England Journal of Medicine.339(8): 520-532.
- Nascimento, G. G. F., Locatelli, J. Freitas, P. C. and Silva, G. L. (2000). Antibacterial activity of plant extracts and phytochemicals on antibiotic resistant bacteria. Brazilian Journal of Microbiology. 31:247-256.
- Ogunsile, A. A. O. (2004). Characterization and selection of Lactobacillus species as probiotics for the control of infantile gastroenteritis. PhD thesis, University of Ibadan, Nigeria.
- Okeke, I. N. and Nataro, J. P. (2001). Enteroaggregative Escherichia coli. The Lancet: Infectious Diseases.1:304-307.
- Okunlola, A., Adewoyin, B. A. and Odeku, O. A. (2007). Evaluation of pharmaceutical and microbial qualities of some herbal medicinal products in South Western Nigeria. Tropical Journal of Pharmaceutical Research, 6(1): 661-670.
- Onyambu, M. O., Chepkwony, H. K., Thoithi, G. N., Ouya, G. O. and Osanjo, G. O. (2013). Microbial quality of unregulated herbal medicinal products in Kenya. African Journal ofPharmacology and Therapeutics. 2(3): 70-75.
- Prescott, L., Harley, J. P. and Klein, D. A. (2005). Microbiology. 6th edition pp.501-502.WCB/McGraw Hill, USA.
- Rajapandian, G., Narayanan, N., Maheswaran, A. and Chanchal, P. (2013). Dangerous world of counterfeit drugs – Pharmacist's role and its prevention. International Journal of Pharmaceutical Sciences and Research. 4(5): 1709-1713.
- Rowe-Tatit, C. R., Shubin, Y. S., Angel, R. and Ligler, F. S. (2004). Detection of Salmonellaenteriticaservophimurium by using a rapid-array based immunosensor. Applieed Environmental Microbiology.69:152-158.
- Varnam, A. H. and Evans, M. G. (1991). Foodborne Pathogens - An Illustrated Text. Wolfe Publishing, London, England.
- World Health Organization (WHO) (2003). General guidelines on good agricultural and field collection practices (GACP) for medicinal plants. World Health Organization, Geneva.
How to Cite
Oluwasegun, A. F., & Modupe, N. V. (2017). Microbial Assessment of Herbal Products in Ota And Its Environs. Nigerian Journal of Microbiology, 31(2), 3914-3919. https://doi.org/10.67614/njm.2017.1u2l54bf
A. F. Oluwasegun, and N. V. Modupe, "Microbial Assessment of Herbal Products in Ota And Its Environs," Nigerian Journal of Microbiology, vol. 31, no. 2, pp. 3914-3919, December 2017. doi: 10.67614/njm.2017.1u2l54bf