Research Article

Microbiology of Semen from Men with Fertility Problems in Imo State, Nigeria

Nwofor C. N1*, Uwaezuoke J. C.2, Okoro C. L2, Ohalete C.N2, Ogbulie J.N3
1 Department of Microbiology, Imo State University Owerri, Nigeria
2 Department Of Microbiology, Faculty of Science, Imo State University, P.M.B. 2000, Owerri, Nigeria.
3 Department of Microbiology,School of Science,Federal University of Technology, Owerri, Imo State,Nigeria.
* Corresponding author: chiomanwofor@gmail.com
Published: Jun, 2017
DOI: 10.67614/njm.2017.2b8ulb2l
Pages: 3649-3652

Abstract

This work is aimed at determining whether presence of microorganisms in seminal fluid might be the cause of male infertility.Three hundred and eighty-eight semen samples were submitted and analyzed microbially by routine semen analysis and culture techniques. Bacteriological examination of the semen samples revealed five bacterial isolates, including Staphylococcus aureus (39.2%), Klebsiella sp. (13.7%), Escherichia coli (9.8%), Proteus mirabilis (15.7%) and Neisserria gonorrhoeae (8.8%). The motility of sperm cells showed that 208 (53.6%) had 70 -100% actively motile cells, 116 (29.9%) had 50-60% actively motile cells and 64 (16.5%) had below 50% actively motile cells. Analysis of the duration of sperm motility showed that 20 (5.2%) had duration of 1- 10 hours, 276 (71.1%) had duration of 11 – 20 hours and 60 (15.5%) had duration of 21 – 24 hours. Analysis of the semen viscosity showed that 16 (4. 1 %) had highly viscous semen, 100 (25. 8%) had slightly viscous semen, 100 (25.8%) had watery semen and 172 (44. 3%) had moderately viscous semen. This study revealed that, infertility in men could be as a result of the presence of microorganisms in semen.

Keywords

Semen bacterial isolates men fertility problem Imo State

References

  1. Auroux MR Jacques C, Mathieu D, Auer J (1991).Is the sperm bacterial ratio a determining factor in impairment of sperm mortality: an in vitro study in man with Escherichia coli. Internat. J. Androl. 14(4): 225-229.
  2. Caldwell J.C., Caldwell P (1978). "A theory of fertility, from Platean to demographic" population and development review No 4.
  3. Carison E, Griwemann A, Keiding N (1992) Evidence for decreasing quality of semen during past 50years.Bio med.J, 305: 609-13.
  4. Carr, D.W, Uvsdmo, M.C. and Acott, T.S. (1983). Effects of pH, Lactate and viscolidase drag on sperm motility: a species comparison. Biology of Reproduction 33: 588-595
  5. Cheesbrough, M (2002).Medical laboratory manual for tropical countries Vol 2.Microbiology.
  6. Ekhaise F.O and Richard F.R.(2008).Common bacteria isolates associated with semen of men complaining of infertility in UB.T.H Benin City Nigeria.World Jhed Sci 3(1):28-33.
  7. Gomez C.I, Stanback W.A., James A.N, (Priwell B.S. Williams R.P (1979). Attachment of Neisseriagonorrhoeae to human sperm. Microscopial study of the effect of trypsin and iron. Brit. J Ven. Dis. 55: 245-255
  8. Guvton A (1992). Human Physiology and Mechanism of Diseases (7th edition). W.B. Saunders publishers, p.690
  9. Holt. J.G. Krieg, N.R, Sneath, P.P.A, Stanley J.T. and Williams S.T. (1996), Bergy's manual of determinative bacteriology. Baltimore, Williams and Wilkins publishing in Maryland.
  10. Huwe P, Diemer T, Ludwig M, Liu J, Schiefer HG, Weidner W. (1998). Influence of different uropathogenic microorganisms on human sperm parameters in an in-vivo experiment.Andrology, 30(supp1 1): 55-9.
  11. Ikeagwu I J, Amadi E.S , Iroha I.R(2008).Antibiotic Sensitivity Pattern of Staphylococcus aureusin Abakaliki Nigeria. Pak J med Sci 24(2):231-235.
  12. Ingermann R.L, Holcomb M, Robinson M.L. and Cloud T.G. (2002).CarbondioxidepK pH affect sperm motility of white sturgeon. J experimental biology.
  13. Merino G, Carranza L.S, Murrieta S, Rodriguez L, Cuervac and Morna L (1995)? Arch Androl 35 (7): 43-7.
  14. Obiajuru I.O.C. and Orumba U.O (2009) Laboratory method for medical microbiology and parasitology. Life way analysationsOwerriISBN: 2277107. P 183.
  15. Okonofua F.E. Dinna Harrish, AdetunwoOdabiye, Thomas K and Rachel .C.Snow9 (1997) The social meaning of infertility in south-west Nigeria. Transition Review 7: 20-220.
  16. Okonofua F.E (1997). Infertility and women's reproductive health in Africa, Afr. J. Reprod. Health, 13 (1):7-8.
  17. Romaniuk A (1998), "Infertility in Tropical Africa". In Okonjo, O and Caldwell, J.T. The population of Tropical Africa. Longman Press, London, P. 233.
  18. Sancka-Maciejewska D, Ciupinska M, and E, Kurpisz (2005) Ureaplasma Urealyticum, Ureaplasma parvum, Mycoplasma Hominis and Mycoplasma genitalium infections and semen quality of infertile men. J reproduimmunol7(1-2): 51-6.
  19. Stephen PB, Russel OD, David FR (1985), Current problems in obstetrics, gynaecology and fertility. Year book medical publishers, Incorporated P. 199.
  20. World Health Organization (2010) WHO laboratory manual for the examination of Human Semen and sperm cervical mucus interaction. 4th ed. Cambridge University press.
How to Cite

N, N. C., C., U. J., L, O. C., C.N, O., & J.N, O. (2017). Microbiology of Semen from Men with Fertility Problems in Imo State, Nigeria. Nigerian Journal of Microbiology, 31(1), 3649-3652. https://doi.org/10.67614/njm.2017.2b8ulb2l

N. C. N, U. J. C., O. C. L, O. C.N, and O. J.N, "Microbiology of Semen from Men with Fertility Problems in Imo State, Nigeria," Nigerian Journal of Microbiology, vol. 31, no. 1, pp. 3649-3652, June 2017. doi: 10.67614/njm.2017.2b8ulb2l

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