Research Article

MICROFLORA OF THE SHELLS OF READY-TO-EAT CONOPHOR (Tetracarpidium conophorum) NUTS ON RETAIL SALE IN OWERRI

Emeka Nwabunnia1*, Chiotna C. Otogbor1
1 Department of Microbiology, Chukwuemeka Odumegwu Ojukwu University, Uli Campus, PMB 02, Uli, Anambra State, Nigeria
* Corresponding author: alcamow@yahoo.com
Published: Dec, 2015
DOI: 10.67614/njm.2015.mxgcpfoz
Pages: 2964- 2971

Abstract

A study was undertaken to determine the bacterial population and diversity of the shells of ready-to-eat conophor (Tetracarpidium conophorum ) nuts retailed in Owerri, Imo State, Nigeria. Fifty samples (5 nuts per sample) of ready-to-eat conophor nuts were purchased from 50 retailers in Ekeonunwo market (EM), Okigwe road motor park (ORMP), Arugo motor park (AMP), New market (NM) and Relief market (RM) and analyzed for total coliform, total aerobic heterotrophic bacteria (TAHB), and the presence of indicator and pathogenic bacteria using standard plate count methods. It was discovered that samples from RM and AMP accounted for the highest and lowest TAHB values of 6.30 and 4.20 logio cfu/g, respectively while total coliform counts in the samples ranged from 1.04 logio cfu/g for RM to 4.18 logio cfu/g for EM. Bacterial species associated with the samples were identified as Salmonella sp., Shigella sp., Enterobacter sp., Klebsiella sp., Bacillus sp., Staphylococcus aureus, Lactobacillus sp., Escherichia coli, Diphtheroids and Actinomycetes. The predominant bacterial isolates were Staphylococcus aureus and Lactobacillus sp. with the later being more predominant. Samples from AMP and ORMP showed E. coli contamination. Salmonella sp. and Shigella sp. were found in samples from AMP, EM and RM. Enterobacter sp. and Klebsiella sp. were confirmed in samples from NM, RM and EM. The presence of indicator of feacal contamination and pathogenic bacteria in the shells of ready-to-eat conophor nuts poses potential risks to the consuming public. The results emphasized the need for appropriate cooking of the nuts to ensure decontamination, maintenance of good hygienic practices during preservation, selling and handling prior to consumption of conophor nuts.

Keywords

Bacterial population and diversity conophor nut shells Owerri

References

  1. Adebesin, A. A., Saromu, O. T., Amusa, N. A. and Fagade, S. O. Microbiological quality of some groundnut products hawked in Bauchi, a Nigerian City. J. Food Technol Afr. 6(2): 53–55.
  2. Adebona, M. B., Ogunusa, A. O. and Oloqunde, A. M. O. (1988). Development of conophor nut-based cereal snack food 1 – Biscuits. J. Food Agric. 2:123–126.
  3. Akpuaka, M. U. and Nwankwo, E. (2000). Analysis and utilization of a drying oil from Tetracarpidium conophorium. Biores. Technol. 73:195–196.
  4. Beuchat, L. R. (1973). Escherichia coli on pecans: Survival under various storage conditions and disinfection with propylene oxide. J. Food Sci. 38: 1063–1065.
  5. Beumer, R. R. (2001). Microbiological hazards and their control-Bacteria. In: Adams, M. R. and Nout, M. J. R (eds). Fermentation and food safety. USA Aspen Publication, Inc., Gaithersburg, Maryland, USA, pp. 141–157.
  6. Bryan, F. I. and Bartleson, C. A. (1985). Mexican style food service operations. Hazard analysis, critical control points and monitoring. J. Food Prot. 48: 509–524.
  7. Collins, C. H., Lyne, P. M., Grange, J. M. and Falkinnam, J. O. (2004). Collins and Lyne's microbiological methods, 8th Edition. Hodder Arnold Publication, New York, pp. 464.
  8. Edem, C. A., Dosuntmu, M. I. and Bassey, F. I. (2009). Determination of proximate composition, ascorbic acid and heavy metals content of Africa walnut (Tetracarpidium canophorum). Pak. J. Nutr. 8 (3): 225–226.
  9. Enujigha, V. N. (2003). Chemical and functional characteristics of conophor nut. Pak. J. Nutr. 2(6): 335–338.
  10. Frazier, W. C. and Westhoff, D. C. (2000). Food microbiology, 4th edition. Tata McGraw-Hill Publication Inc., New Delhi, pp. 17–34.
  11. Hall, H. E. (1971). The significant of Escherichia coli associated with nut meats. Food Technol. 25: 230–323.
  12. Holt, J. G. (ed.) (1982). The shorter Bergey's manual for determinative bacteriology, 8th edition. The Williams and Wilkins Inc., Baltimore, Maryland, USA, pp. 45–50.
  13. Kokal, D. (1965). Viability of Escherichia coli on English walnut mats (Juglans regia). J Food Sci. 30: 325–332.
  14. Little, C. L., Rawal, N., de Pinna, E. and McLauchlin, J. (2009). Survey of Salmonella contamination of edible nut kernels on retail sale in the UK. Food Microbiol. 27 (2): 171–174.
  15. Menseh, P., Yeboah-Manu, D., Owusu-Darko, K., and Ablordey, A. (2002). Street food in Accra, Ghana: How safe are they? Bull. WHO 80: 546–554.
  16. Nester, E. W., Anderson, D. G., Roberts, E. C., Pearsall, N. N. and Nester, M. T. (2004). Microbiology Text: A human perspective, 4th edition. McGraw-Hill Publication, New York, pp. 613–617.
  17. Nwokolo, E. (1987). Composition and availability of nutrients in some tropical grains and oil seeds. Nutr. Rep. Intl 36: 631–640.
  18. Ogunsua, A. O. and Adebona, M. B. (1983). Chemical composition of Tetracarpidium conophorium. J. Food Chem. 10. 173–177.
  19. Oke, O. L. (1995). Leaf protein research in Nigeria. 2nd edition. University of Ibadan Press, Ibadan, Nigeria, pp. 89–94.
  20. Oke, O. L. and Fafunso, M. A. (1975). Lesser known oilseeds: The nutritive value of conophor seeds in vitro. Nutr Rep Intl 12: 41–49.
  21. Oladiji, A. T., Abodunrin, T. P. and Yakubu, M. T. (2009). Toxicological evaluation of Tetracarpium conophorium nut oil-based diet in rats. J. Food Chem. Toxicol. 48(12): 868–902.
  22. Riyaz-UL-Hassan, S., Verma, V., Malik, A. and Qazi, G. N. (2003). Microbiological quality of walnut kernels and apple juice concentrate. Wld J. Microbiol. 19 (6): 845–850.
  23. Vural, A. and Erkan, M. E. (2008). The research of microbiological quality in some edible nut kinds. J. Food Technol. 6(1): 25–28.
How to Cite

Nwabunnia, E., & Otogbor, C. C. (2015). MICROFLORA OF THE SHELLS OF READY-TO-EAT CONOPHOR (Tetracarpidium conophorum) NUTS ON RETAIL SALE IN OWERRI. Nigerian Journal of Microbiology, 29(2), 2964- 2971. https://doi.org/10.67614/njm.2015.mxgcpfoz

E. Nwabunnia, and C. C. Otogbor, "MICROFLORA OF THE SHELLS OF READY-TO-EAT CONOPHOR (Tetracarpidium conophorum) NUTS ON RETAIL SALE IN OWERRI," Nigerian Journal of Microbiology, vol. 29, no. 2, pp. 2964- 2971, December 2015. doi: 10.67614/njm.2015.mxgcpfoz

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