Research Article

Monitoring of Metabolic Compounds from Degradation of Petrochemicals using Indigenous Consortium of Pseudomonas Strains

Ayogu, C.V1*, Ifeanyi, V.O.2, Obasi, N. P.1
1 Aquatic Bioresources Training Centre, Adiabo, National Biotechnology Development Agency, Nigeria.
2 Department of Microbiology, Michael Okpara University of Agriculture Umudike, Abia State.
* Corresponding author: ayogucv@nsmjournal.org.ng
Published: Dec, 2020
DOI: 10.67614/njm.2020.shhfx7p0
Pages: 5221-5238

Abstract

This study was performed to examine the potential of indigenous consortium of Pseudomonas  strains by assessing the metabolic compounds from degradation of petrochemical contaminated soil.  Native microorganisms were isolated using standard microbiological procedures and molecular  identification technique. The physiochemical analysis was conducted using standard laboratory procedure  of American Public Health Association (APHA). Bioremediation of Total Petroleum Hydrocarbon (TPH)  and assessment of metabolic compounds from degradation of petrochemicals were by gravimetric  technique. The identified bacteria were Pseudomonas aeruginosa strain PAER4 119, Pseudomonas  mendocina strain NK-01 and Pseudomonas putida strain B6-2. Observations revealed that the  physiochemical properties were affected due to high pollution level. Highest percentage reduction of the  TPH was recorded at 74.5 9% by P. putida, 67.57 % by P. aeruginosa, 61.62 % by P. mendocina, and  80.81 % by consortium. Also highest percentage reduction of the metabolic compounds showed; 62%, 75  %, 68 %, and 81% for saturated hydrocarbon, 86.25 %, 87.50 %, 91 % and  92 % for phenolic compound,  94.06 %, 95.05 %, 96.53 %, and 97.03 % for asphaltene and polar compound, and 88.89 %, 94.44 %,  94.42%, and 97.22% for aromatic compound. The percentage reductions recorded were achieved by P.  mendocina, P. putida, P. aeruginosa, and the consortium respectively. This study shows that the TPH and  their metabolic compounds were highly degraded. These strains and the assessment technique can be  employed in biodegradation of petrochemical contaminated environment and also in the monitoring of  biodegradation studies

Keywords

Biodegradation metabolic compounds petrochemical contaminated soil physicochemical parameters and Pseudomonas strains

References

  1. Akubugwo, E.I., Ogbuji, C., Chinyere, C.G. and Ugbogu, E.A. (2009). Physicochemical properties and enzymes activity studies in a refined oil contaminated soil in Isukwuato, Abia state, Nigeria. Biokemisti 21: 79-84.
  2. Alexander, M. (2000). Aging bioavailability, and overestimation of risk from environmental pollutants. Environ. Sci. and Tech. 34(20):4259-4265.
  3. Alkio, M., Tabuchi, T.M., Wang, X. and Colon-Carmona, A. (2005). Stress responses to polycyclic aromatic hydrocarbons in Arabidopsis include growth inhibition and hypersensitive response like symptoms. J. Experim. Botan. 56(421):2983-2994.
  4. American Public Health Association (APHA) (2004). Standard methods for the examination of water and waste water. 21st Edition, American Public Health Association, Washington
  5. Bona, C., Rezende, I., Santos, G. and Souza, L. (2011). Effect of soil contaminated by diesel oil on the germination of Schinus terebinthifolius. Brazilian Archives of Biol. Technol. 54(6):1379-1387.
  6. Bundy, J.G., Paton, G.I and Campbell, C.D. (2002). Microbial communities in different soil types do not converge after diesel contamination. J. Appl. Microbiol. 92: 276-288.
  7. Cai, M., Yao, J., Yang, H., Wang, R. and Masakorala, K. (2013). Aerobic biodegradation process of petroleum and pathway of main compounds in water flooding well of Dagang oil field. Biores. Technol. 144: 100–106.
  8. Calvo, C., Manzanera, M., Silva-Castro, G.A., Uad, I. and Gonzalez-Lopez, J. (2009). Application of biosurfactants in soil bioremediation processes. Future prospects. Sci. Total Environ. 407: 3634–3640.
  9. Da Cruz, F.G., De Vasconcellos, P.S., Angolini, F.F.C., Dellagnezze, M.B., Garcia, N.S.I., De Oliveira, M.V., Neto, S.D.V.E. and Marasoli, A.J. (2011). Could Petroleum Biodegradation Be a Joint Achievement of Aerobic and Anaerobic Microorganisms in Deep Sea Reservoirs? AMB Express 1:47
  10. Das, K. and Mukherjee, A.K. (2007). Crude Petroleum-Oil Biodegradation Efficiency of Bacillus subtilis and Pseudomonas aeruginosa Strains Isolated from Petroleum-Oil Contaminated Soil from North-East India. Biores. Technol. 98: 1339-1345.
  11. Ekundayo, E.O. and C.O. Obuekwe, (1997). Effects of an oil spill on soil physicochemical properties of a spill site in a typic paleudult of midwestern Nigeria. Environ. Monitor. Assess. 45: 209-221
  12. FAO/IIASA/ISRIC/ISSCAS/JRC, (2008). Harmonized World Soil Database (version 1.0). FAO, Rome, Italy and IIASA, Laxenburg, Austria.
  13. Garrity, G.M., Brenner, D.J., Krieg, N.R. and Staley J.T. (2005). Bergey's Manual of Systematic Bacteriology (second ed.), Springer-Verlag, New York, 323–359.
  14. Ghazali, F.M., Rahman, R.N.Z.A., Salleh, A.B. and Basri, M. (2004). Biodegradation of hydrocarbons in soil by microbial consortium. Biores. Technol. 54: 61–67.
  15. Habibarata, T., and Kristanti, R.A. (2012). Identification of metabolites from benzo[a]pyrene oxidation by ligninolytic enzymes of Polyporus sp. S133. J. Environ. Managem. 115: 119–125.
  16. Haramaya, S., Sugiura, K., Ishihara, M. and Shimauchi, T (1997). Physicochemical properties and biodegradability of crude oil. Environ. Sci. Technol. 31: 45-51.
  17. Head, I.M., Jones, D.M. and Röling, W.F.M. (2006). Marine Microorganisms Make a Meal of Oil. Nat. Rev. Microbiol. 4: 173-182
  18. Holt, J.G., Krieg, N.G., Sneathm .P.H.A., Staley, J.T. and Williams, S.T. (1994). Bergey's Manual of Determinative Bacteriology, 9th edn. Williams and Williams.
  19. Holt, J.G., Krieg N.R., Sneath P.H.A., Staley, J.T. and Williams, S.T. (1998). Bergey's Manual of Determinative Bacteriology. Baltimore, M.D., USA: 9th Edition, Williams and Williams.
  20. Jirasripongpun, K. (2000). The characterization of oil-degrading microorganisms from lubricating oil contaminated (scale) soil. 35(4):296-300.
  21. Joutey, N.T., Bahafid, W., Sayel, H. and ElGhachtouli, N. (2013). "Biodegradation: involved microorganisms and genetically engineered microorganisms," in Biodegradation – Life of Science, Chap. 11, eds Chamy, R. and Rosenkranz, F. (Rijeka: Intech).
  22. Ketler, T.A., Doran, J.W., and Gilbert, T.L. (2001). Simplified method for soil particle size determination to accompany soil quality analyses. Soil Sci. Soc. America J. 65: 849-82.
  23. Kibane, J., Rangantahan, R., Cleveland, L., Kayser, K.J., Ribero, C. and Linhares, M.M. (2000). Selective Removal of Nitrogen from Petroleum by Pseudomonas sp. Strain IGTN 9M. Appl. Environ. Microbiol. 66: 688-693.
  24. Kim, J.S. and Crowley, D.E (2007). Microbial Diversity in Natural Asphalts of the Rancho La Brea Tar Pits. Appl. Environ. Microbiol. 73(14):4579-4591.
  25. Kostal, J., Suchanek, M., Klierova, H., Demnerova, K., Kralova, B. and McBeth, D.L. (1998). Pseudomonas C12B, an SDS degrading strain, harbours a plasmid coding for degradation of medium chain length n-alkanes. Int. Biodeterior. Biodeg. 42(4):221-228.
  26. Lin, Q. and Mendelssohn, I. (2009). Potential of restoration and phytoremedion with Juncus roemerianus for diesel contaminated coastal wetlands. Ecol. Eng.35: 89-91.
  27. Lin, H.F., Traver, D., Zhu, H., Dooley, K., Paw, B.H., Zon, L.I., and Handin, R. (2005). Analysis of thrombocyte development in CD41-GFP transgenic zebrafish. Blood 106(12):3803-3810.
  28. Luepromchai, E., Lerthamrongwak, W., Pinphanichakran, P., Thaniyavarn, S., Pattaragulwanit, K. and Juntongjin, K. (2007). Biodegradation of PAHs in petroleum-contaminated soil using tamarind leaves as microbial inoculums. Songklanakarin J. Sci. Technol. 29: 515-527.
  29. Malia, M.P. and Coote, T.E. (2002). Germination of Lipidium sativum as a method to evaluate polycyclic aromatic hydrocarbons (PAHs) removal from contaminated soil. Int. Biodeterior. Biodeg. 50: 107-113
  30. Manal, E. A. (2011). Biodegradation of crude oil by bacteria isolated from produced formation water on onshore oil field in Sudan, University of Khourtum, Sudan.
  31. Mander, L.N., and Williams, C.M. (2003). Oxidative degradation of benzene rings. Tetrahedron 59(8):1105-1136
  32. Margesin, R. and Schinner, F. (2001). Biodegradation and Bioremediation of Hydrocarbons in Extreme Environments. Appl. Microbiol. Biotechnol. 56: 650-663.
  33. Mishra, S., Singh, S.N and Pande, V. (2014). Bacteria induced degradation of flourathene in minimal salt medium mediated by catabolic enzymes in vitro condition. Bioresour. Technol. 164:299-308.
  34. Nnaji, G.U., Mbagwu, S.C and Asadu, C.A (2005). Changes in physical properties of soil under cassava monocropping and organic and inorganic soil amendments. Proceedings of the 19th Annual Conference of Farm Management Association of Nigeria (FAMAN05), October 18-20, 2005, Delsu, Asaba Campus, Delta State, pp: 374-377.
  35. Nogueira, L., Inckot, R., Santos, C., Souza, L., Bona, C. (2011). Phytotoxicity of petroleum contaminated soil and bioremediated soil on Allyphus edulis. Rodriguesia 62(3):459-466.
  36. Nwaogu, L.A and Onyeze, G.O.C. (2010). Environmental impact of Gas Flaring on Ebocha-Egbema, Niger-Delta, Nigeria. Nigeria J. Biochem. and Molec. boil. 25(1):25-30.
  37. Oberhofer, T.R., Rowen, J.W and Cunningham, G.F. (1999). Characterization and identification of gram-negative, nonfermentative bacteria. J. Clin. Microbiol. 5: 208-210.
  38. Oliveira, P.L.F., Vasconcellos, S.P., Angolini, C.F.F., da Cruz, G.F., Marsaioli, A.J., Neto, E.V.S. and Oliveira, V.M. (2012). Taxonomic Diversity and Biodegradation Potential Bacteria Isolated from Oil Reservoirs of an Offshore Southern Brazilian Basin. J. Petrol. Bioresour. Biotechnol. 3: 132.
  39. Onyeike, E.W., Ogbuja, S.I. and Nwainuka, N.M. (2000): Inorganic ion levels of soils and streams in some areas of Ogoni land, Nigeria as affected by crude oil spillage. Environ. Monito. Assess.73: 1991-2125.
  40. Osuji, L.C. (2006). Petroleum Industry Effluents and Other Oxygen-Demanding Wastes in Niger Delta, Nigeria. Chem. and Biodiv. 3(7): 705-717.
  41. Osuji, L. and Nwoye, I. (2007). An appraisal of the impact of petroleum hydrocarbons on soil fertility: the Owaza experience. Afr. J. Agric. Res. 2: 318-324.
  42. Ozen, A. E, and Ussery, D. W. (2012). Defining the Pseudomonas Genus: Where Do We Draw the Line with Azotobacter? Microb. Ecol. 63(2):239–248.
  43. Pathak, H., Saharawat, Y.S., Gathala, M., & Ladha, J.K. (2011). Impact of resource-conserving technologies on productivity and greenhouse gas emission in rice-wheat system. Greenhouse Gases: Sci Technol. 1: 261-277.
  44. Plaza, G.A., Lukasik, K., Wypych, J. Nal¸cz-Jawecki, G., Berry, C. and Brogmon, R.L. (2008). Biodegradation of crude oil and distillation products by biosurfactant-producing bacteria. Polish J. of Environ. Stud. 1: 87-94.
  45. Public Health England, (2015). Identification of Pseudomonas species and other non-Glucose Fermenters. Bacteriology-Identification. Microbiol. Serv. Stand. Unit. 3: 1-31.
  46. Rahman, K.S.M., Rahman, T.J.I aksharnapaeranualsamy, P. and Banat, I.M. (2002). Towards efficient crude oil degradation by a mixed bacterial consortium. Biores. Technol. 85: 257–261.
  47. Rui, L., Rajendrasinh, N.J, Qixing, Z. and Zhe L. (2012). Treatment and Remediation of Petroleum-Contaminated Soils Using Selective Ornamental Plants. Environ. Engin. Sci. 26:
  48. Reynoso-Cuevas, L., Gallegos Martinez, M.E., Cruz Sosa, F., Gutierrez Rojas, M. (2008). In vitro evaluation of germination and growth of five plant species on medium supplemented with hydrocarbons associated with contaminated soils. Bores. Technol. 99(14):6379-6385.
  49. Saadoun, I. (2000). Isolation and characterization of bacteria from crude petroleum-oil contaminated soil and their potential to degrade diesel fuel. J. Basic Microbiol. 40: 420-428Salleh, A.B., Mohamad, Ghazali, F., Abd Rahman, R.N.Z. and Basri, M. (2003). Biodegradation of petroleum hydrocarbon pollution. Ind. J. Biotechnol. 2: 411-425.
  50. Sambrook, J. and Roussel, D.W. (2001). Molecular Cloning: A Laboratory Manual Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York.
  51. Sathishkumar, M., Binupriya, A.R., Baik, S.H and Yun, S.E. (2008). Biodegradation of Crude Oil by Individual Bacterial Strains and a Mixed Bacterial Consortium Isolated from Hydrocarbon Contaminated Areas. Clean, 36: 92-96.
  52. Tao, X.-Q., Lu, G.-N., Dang, Z., Yang, C., and Yi, X.-Y. (2007). A phenanthrene-degrading strain Sphingomonas sp. GY2B isolated from contaminated soils. Process Biochem. 42(3):401–408.
  53. Thapa, B. Kumar, A.K.C and Ghimire, A. (2012). A review on bioremediation of petroleum contaminants in soil. Kathmandu University J. Sci., Engin. Technol. 8(1):164-170.
  54. Uche, O.N., Owhondah, W.M. and Augustine, U.A. (2011). The Omoku oil pipeline oil spill: Total hydrocarbon content of affected soils and the impact on the nutritive value of food crops. Archiv.Appl. Sci. Res. 3: 514-521.
  55. Walkley, A. and Black, I.A. (1999). An examination of the Degtjareff method for determining organic carbon in soils: Effect of variations in digestion conditions and of inorganic soil constituents. Soil Sci. 63: 251-263.
  56. Yergeau, E., Sanschagrin, S., Beaumier, D. and Greer, C.W. (2012). Metagenomic Analysis of the Bioremediation of Diesel-Contaminated Canadian Arctic Soils. PLoS ONE 7(1):e30058.
  57. Yan, Z., Song, N., Cai, H., Tay, J.H and Jiang, H. (2012). Enhanced degradation of phenanthrene and pyrene in freshwater sediments combined employment of sediment microbial fuel cell and amorphous ferric hydroxide. J. Hazard Mater. 199: 217-225.
  58. Yu, H., Zhu, I. and Zhou, W. (2007). Enhanced desorption and biodegradation of phenanthrene in soil-water systems with the presence of anionic-nonionic mixed surfactants. J. Hazard Mater. 142(1-2)354-361.
  59. Zhang, Y. (2008). Geochemical kinetics. Princeton University Press.
  60. Zhang, Z., Hou, Z., Yang, C., Ma, C., Tao, F. and Xu, P. (2011). Degradation of n-alkanes and polycyclic aromatic hydrocarbons in petroleum by a newly isolated Pseudomonas aeruginosa DQ8. Biores. Technol. 102(5):4111-4111.
How to Cite

C.V, A., V.O., I., & P., O. N. (2020). Monitoring of Metabolic Compounds from Degradation of Petrochemicals using Indigenous Consortium of Pseudomonas Strains. Nigerian Journal of Microbiology, 34(2), 5221-5238. https://doi.org/10.67614/njm.2020.shhfx7p0

A. C.V, I. V.O., and O. N. P., "Monitoring of Metabolic Compounds from Degradation of Petrochemicals using Indigenous Consortium of Pseudomonas Strains," Nigerian Journal of Microbiology, vol. 34, no. 2, pp. 5221-5238, December 2020. doi: 10.67614/njm.2020.shhfx7p0

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