Research Article

MORPHOGENESIS OF ORAL AND GENITOURINARY TRACT ISOLATES OF Candida albicans STRAINS AS INFLUENCED BY DIFFERENT GROWTH CONDITIONS

Uzoechi A. U1*, Vwaezuoke J,C,1, Nwachukwu I. O1, Ahuocha Pauline1, Nwachukwu M.J1
1 Department of Microbiology, Imo State University Owerri Imo State
* Corresponding author: uche4realab@yahoo.com
Published: Dec, 2015
Pages: 3039-3037

Abstract

Growth pattern and mycelia formation of two strains O (Oral isolate) and G (genitourinary tract isolate) of Candida albicans in horse-serum, MSB and RPMI 1640 media indicates that the first medium promotes filamentation in both strains while MSB initiates medium mycelium formation for O strain and RPMI stimulates the yeast growth and low filamentation. However, RPMI promotes moderate filamentation and growth of C. albicans (G). The peak of mycelium production appeared between 1.5 to 4.5h after inoculation of C. albicans O and between 1.5 to 6.0 and 7.5h for C. albicans G. the pH value of 7.4 appeared to be optimal for filamentation on MSB at 37°C for C. albicans. O (Oral pH slightly alkaline), While, pH of 4.4 was responsible for highest mycelia formation of C albicans G. (vaginal pH around 4.5) under same cultural conditions incubation temperature of 37°C was concomitant with the highest germ-tube formation by C. albicans O, on MSB at pH 7.4 while 34°C was optimum for filamentation by C. albicans G. on MSB at pH 4.4. These results indicated that the pattern of filamentation by C. albicans depends mainly on the yeast strain. The nutritional and cultural conditions control the route of yeast dimorphism. The two C. albicans strains (O and G) showed the same phenotypic switching when grown on solid media of MSB, RPMI, horse serum and blood agar base, in presence of 7%C02 (anaerobic) and in absence of 7%C02 (aerobic) conditions). However C. albicans G showed phenotypic switching on chocolate agar (feet appendages in presence of 7%C02 and normal growth in absence of CO2) while C. albicans O showed no switching under the same conditions.

Keywords

Morphogenesis. Dimorphism. Gefm-tube. Filamentation. Phenotypic switching

References

  1. Cetinkaya, Z. and N. kiraz, (2005). In vitro investigation of antifungal activities of phenotypic variation in Cadida albicans. Strains against fluconazole, intraconazple and voriconale. Jap. J. Med. Mycol., 46: 197-201.
  2. Casanova, M, A. Cervera, D. Gozalbo and J. Martinez, (1997). Hemin induces germ tube formation in Candida albicans. Infect. Immun. 65: 4360-4364.
  3. De Berardis, F, F.A. mulhschlegel, A. Cassane and W.A. fonzi 1998. The pH of the host Niche Controls gene expression in and virulence of Candida albicans. Infect. Immun. 66: 3317-1125.
  4. Evans, E.G. F.C Odds. MD. Richardson and K.T Holland. (2004). The effect of growth medium on filament production in Candida albicans. Sabornaudia 52: 112-119
  5. Evans. E.G F.C Odds, M.D. Richardson and K.T. Holland, (2005). Optimum conditions for initiation of filamentation in Candida albicans. Can. J. Microbiol., 21: 338-342
  6. Ernest, K.F. (2000). Transcription factors in Candida albicans, environmental control of morphogenesis. Microbiol. 146: 1763-1774
  7. Enjalbert, B and M. whiteway, 2005. Release from quorum sensing molecules triggers hyphal formation during Candida albicans resumption of growth. Eukaryote, 4: 1203-1210.
  8. Eggimann, P. J. Garbino and D. PITTET, (2003). Epidemiology of Candida species infection in critically-ill immunosuppressed patients. Lancet Infect. Dis. 3: 685-702.
  9. Gow. N.A (2002). Cell biology and cell cycle of Candida. In Calderone, R.A. (ed.) Candida and candidiasis ASM. Press Washington. D.C, PP: 145-158.
  10. Kaur, S.P Mishra and R. Prasad, (1988). Dempsion associated changes in ami 50: 97-100.
  11. Lee, K.L., H.R. Buckley and C.C Campbell, (2005). An amino acid liquid synthetic medium for the development of mycelia and yeast forms of Candida albicans. Sabouraudia, 148-153.
  12. Odds. F.C, (1988). Candida and candidiasis, 2nd ed. Bailliere Tendal, London. United Kindom, pp:42-59
  13. Paranjape, V. and A. Datta, (1991). Role of nutritional status of the cell in pH regulated dimorphism in candida albicans. FEMS Microbiol. Let. 80: 333-336.
  14. Saporito - Irwin S.M., C.E. birse. PS. Sypherd and W.A fanza (1995). PHRI, a pH – regulated gene of Candida albicans. Is required for morphogenesis. Mol. Cell. Bio., 15: 601-613.
  15. Sell. D.R. (2002) Candida. Commensalisms and virulence: the evolution of phenotypic plasticity. Acta Tropica., 81:101-110
  16. Slutsky, R.J Buifo and D R. Sall. (2005). High frequency switching of colony morphology in Candida. albicans. J. Biol 230:660-669
  17. Yang, Y.L. (2003) virulence factors of Candida species. J. Microbiol. Immunol. Infect., 36: 223-228.
How to Cite

U, U. A., J,C,, V., O, N. I., Pauline, A., & M.J, N. (2015). MORPHOGENESIS OF ORAL AND GENITOURINARY TRACT ISOLATES OF Candida albicans STRAINS AS INFLUENCED BY DIFFERENT GROWTH CONDITIONS. Nigerian Journal of Microbiology, 29(2), 3039-3037.

U. A. U, V. J,C,, N. I. O, A. Pauline, and N. M.J, "MORPHOGENESIS OF ORAL AND GENITOURINARY TRACT ISOLATES OF Candida albicans STRAINS AS INFLUENCED BY DIFFERENT GROWTH CONDITIONS," Nigerian Journal of Microbiology, vol. 29, no. 2, pp. 3039-3037, December 2015.

Share this article:
Facebook X / Twitter LinkedIn
Download PDF Back to Articles