Research Article

Multiple Antibiotic Resistance among Escherichia coli Isolated from Selected Abattoirs in Northwestern Nigeria

Shiaka G.P1*, Yakubu S.E2, Amin Mukhtar M2, Whong, C.M.Z3, Adeleye A.O4, Dashen M.M.5
1 Department of Microbiology and Biotechnology, Federal University Dutse, Jigawa State
2 Department of Microbiology, Ahmadu Bello University, Zaria Kaduna State, Nigeria.
3 Department of Microbiology, Faculty of Life Sciences, Ahmadu Bello University, Zaria, West Africa Nigeria
4 Department of Environmental Science, Federal University Dutse, Jigawa State, Nigeria.
5 Department of Microbiology, University of Jos, Plateau state, Nigeria.
* Corresponding author: shiaka.g@fud.edu.ng
Published: Dec, 2020
DOI: 10.67614/njm.2020.9h9tamsd
Pages: 5351-5359

Abstract

Escherichia coli is one of the major contaminants in the abattoir because of its frequent  association with both living and cattle carcasses. It is used as indicator for both contamination and  prevalence of antibiotic resistance. Samples were collected from water, effluent and swabs of various  surfaces from selected abattoirs in northwestern Nigeria. They were analyzed using microbiological  techniques for isolation of E. coli. Fifty of these bacteria were randomly selected and tested against nine  selected antibiotics: amoxicillin-clavulanic acid (30 µg), cefoxitin (30 µg), gentamicin (10 µg), tetracycline  (30 µg), ciprofloxacin (5 µg), sulfonamides and trimethroprim (25 µg), chloramphenicol (30 µg),  vancomycin (30 µg) and erythromycin (15 µg)to determine their level of resistance to each of the  antibiotics using disc diffusion method. The results showed highest resistance of E. coli isolates to  vancomycin (92%), followed by amoxicillin-clavulanic acid (76%) and erythromycin (76%). None of the  isolates was resistant to gentamicin and ciprofloxacin. However, resistance against chloramphenicol (8%),  sulfonamides and trimethroprim (16%), and cefoxitin(24%) were low. Multiple antibiotic resistant index  (MARI) was determined and 46 (92%) of the isolates were found to be multi-drug resistant. Indiscriminate  use of antibiotics for treatment, as growth promoter in animal foods and poor hygiene practices could be  responsible for this level of resistance.  The high resistant E. coli could be a significant threat to public  health due to the risk of transferring the bacteria into food chain hence, monitoring antimicrobial resistance  and virulence is indispensable.

Keywords

Abattoir Antibiotic resistance E. coli Isolation.

References

  1. Ahaduzzaman, M.D., Hassan, M.M, Alam, M., Islam, I and Uddin, I. (2014). Antimicrobial resistance pattern against Staphylococcus aureus environmental effluents. Research Journal for Veterinary Practioners,2(1):13-16.
  2. Ariel, E.S., Padua, G.T., Moreira, C.N., Martins, P.S. and Montes, M.C. (2020). Frequency of antibiotic resistant entropathogenic E. coli in bovine carcasses at a slaughterhouse in Brazil. Research Society and Development, 9(7): 1-15.
  3. Atnafie, B., Paulos, D., Abera, M., Tefera, G. and Hailu, D. (2017). Occurences of E. coli 57:H7 in cattle carcass and contamination of carcass and various contact surfaces in abattoir and butcher shops of Hawassa, Ethiopia. BMC Microbiology, 17(24): 2989.
  4. Bauer, A.W., Kirby, W.M.M., Sherris, J.C and Turch, M. (1996). Antibiotic testing by standardized single disk method. Annual Journal of Clinical Pathology, 45:493-496.
  5. Bok, E., Mazurek, J., Stosik, M., Wojciech, M and Baldy-Chudzik, K. (2015). Prevalence of virulence determinants and antimicrobial resistance among commensal Escherichia coliderived from dairy and beef cattle. International Journal of Environmental Research and Public Health, 12: 970-985.
  6. Caruso, G. (2018). Antibiotic Resistance in Escherichia coli from Farm Livestock and Related Analytical Methods: A Review. J AOAC Inc, 101(4): 916-922.
  7. Cheesbrough, M. (2010). District Laboratory Practice in Tropical Countries. 2nd edn. Cambridge University Press, New York, pp. 35-70, 132-143, 157-234.
  8. Clinical and Laboratory Standards Institute (CLSI). (2013). Performance Standards for Antimicrobial resistance of E. coli to aminoglycosides. Adv. Med. Sci, 63(1): 9-13.
  9. Capita, R. and Alonso-Calleja, C. (2013). Antibiotic-resistant bacteria: a challenge for the food industry. Critical Review Food Science of Nutrition, 53:11-48.
  10. Davies, J. and Davies, D. (2010). Origins and evolution of antibiotic resistance. Microbiology Molecular Biology Reviews,74:417–433
  11. Fair, R. J. and Tor, Y. (2014). Antibiotics and bacterial resistance in the 21st century. Perspecs Medicin Chem, 6: 25-64. doi:10.4137/pmc.s14459
  12. Falodun, O.I. and Ajala, F.A. (2018).Physicochemical Studies of Environmental Samples and Antimicrobial Susceptibility Profile of Escherichia coli isolated from Akinyele Abattoir,Ibadan, Nigeria. Journal of Applied Life Sciences International, 17(2): 1-8
  13. Fontcubetra, M., Planell, R., Torrents, A., Sabaté, S., Gonzalez, R., Ramoneda, M. and Simon, M. (2016). Characterization of Shiga Toxin- Producing Escherichia coli O157 Isolates from Bovine Carcasses. Journal of food protection, 79: 1418-1423.
  14. Krumperman, P.H. (1983). Multiple antibiotic resistance indexing of E. coli to identify high-risk sources of faecal contamination of foods. Applied and Environmental Microbiology, 46(1):165-170.
  15. Leung, E., E Weil, D., Mario, R and Nakatani, H. (2011). World Health Day Antimicrobial Resistance Technical Working Group. Bull World Health Organization, 89:390-392.
  16. Loiko, M.R., De Paula, C.M., Langone, A.C., Rodrigues, R.Q., Cibulski, S., Rodrigues, R.D.O.and Tondo, E.C. (2016). Genotype and antimicrobial resistance of Shiga toxin-producing Escherichia coli O157:H7 recovered from cattle at different stages of slaughtering in southern Brazil. Meat Science, 116:193-200.
  17. Magiorakos, A.P., Srinivasan, A., Carey, R.B., Carmely, Y., Falagas, M.E., Giske, C.G., Harbarth, S., Hindler, J.F., Kahlmeter, G., Olsson-Liljequist, B., Paterson, D.L., Rice, L. B., Stelling, J., Struelens, M.J., Vatopoulos, V, Weber, J.T. and Monnet, D.L. (2012). Multidrug-resistant, extensively drug-resistant and pandrug-resistant bacteria: an international expert proposal for interim standard definitions for acquired resistance. Clinical Microbial Infection, 18:268–281.
  18. Manyi-Loh, C., Mamphweli, S., Meyer, E., and Okoh, A. (2018). Antibiotic Use in Agriculture and Its Consequential Resistance in Environmental Sources: Potential Public Health Implications. Molecules, 23(4): doi:10.3390/molecules23040795
  19. Marshall, B. M., & Levy, S. B. (2011). Food animals and antimicrobials: impacts on human health. Clin Microbiol Rev, 24(4): 718-733.
  20. Murutu, R., Luanda, C., Rugumisa, B., Mwanyika, G., Subbiah, M., Call, D.R. and Buza, J. (2016). Detection of microbial surface contamination and antibiotic resistant Escherichia coli on beef carcasses in Arusha, Tanzania. African Journal of Microbiology Research, 10: 1148-1155.
  21. Mulvey, M. R., and Simor, A. E. (2009). Antimicrobial resistance in hospitals: how concerned should we be? Cmaj, 180(4): 408-415.
  22. Nagai, A.C.S., Pizzolitto, E.L., Bruno, G.S.B and Queiroz, L.C. (2016). Detection of Klebsiella pneumonia and Escherichia coli. Rev.Cien. Farm. Basic. Apl, 37: 1.
  23. Ojdana, D., Sieriko, A., Sacha, P., Wieczorek, P. and Trynizewska, E. (2018). Genetic basis of enzymatic resistance of E. coli to aminoglycosides. Adv. Med. Sci, 63(1): 9-13.
  24. Park, S., Navratil, S., Gregory, A., Bauer, A., Srinath, I., Szonyi, B., Nightingale, K., Anciso, J., Jun, M., Han, D., Lawhon, S. and Ivanek, R. (2014). Farm management, environment, and weather factors jointly affect the probability of spinach contamination by generic Escherichia coli at the preharvest stage. Applied and Environmental Microbiology, 80:2504-2515.
  25. Park, D., Stanton, C., Ciezki, K., Parrell, D., Bozile, M., Pike, D., Forst, S.A., Kozel, K.C. and Ivanek, R. (2013). Evolution of the Stx2-encoding prophage in persistent bovine E coli O157:H7 strains. Applied and Environmental Microbiology, 79:1563–1572.
  26. Pissetti, C, Werlang, G.O, Kich, J.D. and Cardoso, M. (2014). Detection and isolation of Escherichia coli multirresistente enoticipamenteclacionadosemfezese carcaçassuínas. Acta Scieniae Veterinariae,44: 1376.
  27. Rajesh, V.K. (2014). Antimicrobial and Their Proper Use in Livestock: A Case for Global Concern. Rubina, L., Jeyakumar, E. and Thomas, G. (Eds). Narosa pub. House PVT. LTD, pp 253-262.
  28. Rychlik, I, Gregorova D and Hadaka, H. (2006). Distribution and function of plasmids in Salmonella enteric. Veterinary Microbiology, 112(1):1-10.
  29. Shiaka, G.P., Wakili, S., Mansur, O.A. and Odigoi, G.I. (2017). Antibiotic susceptibility profile of E. coli from diarrhoea stool of some children attending General hospital, Dutse, Jigawa state. Dutse Journal of Pure and Applied Sciences, 3(2): 372-380.
  30. Shamsul, B.M.T, Adamu, M.T., Mohd-Desa, M.N., Khairani-Bejo, S. (2016). Prevalence of Escherichia Coli O157:H7 and Enterobacteriaceae on hands of workers in halal cattle abattoirs in peninsular Malaysia. Malaysia J Med Sci.23(5):65-71.
  31. Shehabi, A. A., Odeh, J. F., and Fayyad, M. (2006). Characterization of antimicrobial resistance and class 1 integrons found in Escherichia coli isolates from human stools and drinking water sources in Jordan, J Chemother, 18(5): 468-472.
  32. Shitandi, A. A. and Sternesjo, A. (2004). Prevalence of multidrug resistant S. aureus in milk from large and small-scale producers in Kenya. Journal of Dairy Science, 87:445-4349.
  33. Subramani, S. and Vignesh, S. (2012). Multiple antibiotic resistance index study and multidrug resistance character analysis of a few golden staph isolates. Asian Journal of Pharmacy and Life Science, 2(2):151-154.
  34. Tadesse, D.A., Zhao, S., Tong, E., Ayers, S., Singh, A. and McDermott, P.F. (2012). Antimicrobial Drug Resistance in E. coli from humans and food animals, United States, 1950-2002 pp 18-19.
  35. Tambekar, D.H., Dhanorkr, D.V, Gulhare, S.R, Khadelwal, V.K, and Dudhane, M.N. (2006). Antibiotic susceptibility of some UTI pathogens commonly used antibiotics. African Journal of Biotechnology, 1562-1565.
  36. Tiwari, R.P., Hoondal, G.S. and Tiwari, R. (2009). Laboratory techniques in Microbiology and Biotechnology. Abhishek Pubs. Chandigarh, India. Pp 10-12, 17-21, 51-58.
  37. Togoobaatar, G., Ikeda, N., Ali, M., Sonomjamts, M., Dashdemberel, S. and Mori, R. (2010). Survey on non-prescribed use of antibiotics for children in an urban community in Mongolia. Bull World Health Organization, 88:930-936.
  38. Unamba-Oparah, I.C., Abiade C.U., Chah K.F., Opara M.N. and Okoli C.C. (2012). Detection of Verotoxigenic Escherichia Coli from cattle slaughtered at Nsukka municipal abattoir, South Eastern Nigeria. Journal of Veterinary Advancement,6(2):279-284.
  39. US Food and Drug Administration. (2010). National antimicrobial resistance monitoring system -enteric bacteria (NARMS): 2008 executive report. Rockville MD.
  40. Wheatley P., Giotis, E.S & McKevitt, A.I. (2010). Effects of slaughtering operations on carcass contamination in an Irish pork production plant. Irish Veterinary Journal, 4: 382-388
  41. WHO (2015). Global Action Plan on Antimicrobial Resistance. Retrieved from http:apps.who.int/iris/bitstream/handle/10665/193736/9789241509763_eng.pdf?sequence=1
  42. WHO (2017). Guidelines on use of medically important antimicrobials in food-producing animals. Geneva: World Health Organization. In (Licence: CC BY-NC-SA 3.0 IGO. ed.)
How to Cite

G.P, S., S.E, Y., M, A. . M., C.M.Z, W., A.O, A., & M.M., D. (2020). Multiple Antibiotic Resistance among Escherichia coli Isolated from Selected Abattoirs in Northwestern Nigeria. Nigerian Journal of Microbiology, 34(2), 5351-5359. https://doi.org/10.67614/njm.2020.9h9tamsd

S. G.P, Y. S.E, A. . M. M, W. C.M.Z, A. A.O, and D. M.M., "Multiple Antibiotic Resistance among Escherichia coli Isolated from Selected Abattoirs in Northwestern Nigeria," Nigerian Journal of Microbiology, vol. 34, no. 2, pp. 5351-5359, December 2020. doi: 10.67614/njm.2020.9h9tamsd

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