Research Article

Susceptibility Pattern of Bacteria Isolated from Postoperative Wounds of Hysterectomy Patients in Catholic Hospitals at Umuahia, Abia State, Nigeria

Nwankwo I U1*, Edward K.C1, Nwoba, C.N2, Nwaka, S.O.1
1 Department of Microbiology, Micheal Okpara University of Agriculture, Umudike
2 Medical Laboratory Department, Micheal Okpara University Of Agriculture, Umudike, Medical Centre.
* Corresponding author: immaugo@yahoo.com
Published: Dec, 2020
DOI: 10.67614/njm.2020.6mh762yj
Pages: 5343-5350

Abstract

The study identified the bacterial isolates associated with the postoperative wound of women that underwent a hysterectomy and determined their susceptibility pattern to some antibiotics. Sixty (60) specimens of a postoperative wound from patients that underwent hysterectomy were aseptically collected and cultured using standard microbiological procedures. The isolates were identified using Gram stain and biochemical methods, and disc diffusion method was used to test for the susceptibility pattern of the isolates. Six bacterial isolates, namely Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, Streptococcus specie, Klebsiella species, and Proteus species, were isolated. Amongst the six isolates, Pseudomonas aeruginosa had the highest frequency of occurrence 12(29.3%), followed by Staphylococcus aureus 10(24.4%), Klebsiella specie 8(19.5%), Escherichia coli, 5(12.5%) while the least was recorded against Proteus specie 2(4.9%). Among the Gram-positive bacteria, the high level of resistance was recorded by Streptococcus specie (80%) against penicillin, followed by Staphylococcus aureus (70%). In comparison, the least level of resistance was observed with ampicillin (25%) against Streptococcus specie. The zone of inhibition of the antibiotics against the isolates ranges from 8.7–17.3mm for the Gram negative and 8.2-17.6mm for Gram-positive isolates. Streptomycin and Gentamicin had the highest inhibition (17.3mm) against Gram-negative, while penicillin produces the highest zone of inhibition against the Gram-positive isolates. The study identifies the pronounce resistance of isolates to commonly used antibiotics, which suggested the need for rational use of the drugs to prevent the emergence of multi drug resistant strains. Appropriate infection control measures and sound antibiotic policy are necessary to reduce postoperative wound infections.

Keywords

Susceptibility pattern Postoperative wounds Hysterectomy Bacteria isolates.

References

  1. Agboeze, J. Robinsia, C.O., Odikika, U.J., Paul, O.E. Chukwuemeka, U., Azubuike, K.O., Conrad, E., and Emmanuel, N. (2014). Antimicrobial profile of post cesarean wound infection at Federal Hospital Abakaliki. African Journal of Medical and Health Sciences, 12(2):99-102.
  2. Agrihotu, N., Gupta, U. and Joshi, R.M. (2013). Aerobic bacterial isolates from burn wound infections and their antibiograms: a five year study. Burns, 30: 241-243
  3. Ahmed, F.E. and Wasti, S. (2001). Infection complications following abdominal hysterectomy in Karachi. Pakistan International Journal of Gynecology and Obstetrics, 73: 27 – 34.
  4. Amoran, O.E., Sogebi, A.O. and Fatugase, D.M. (2013). Rates and risk factors associated with surgical site infections in a tertiary care center in south-western Nigeria. International Journal of tropical Disease and Health 3(1): 25 – 36.
  5. Anguzu, J.R. and Olila, D. (2007). Drug sensitivity patterns of bacterial isolates from septic postoperative wounds in a regional referral hospital in Uganda. African Health Sciences, 7(3):148-154.
  6. Armour, A.D., Shankewsky, H.A., Swanson, T; Lee, J. and Tredget, E.E. (2007). The impact of nosocomially acquired resistant Pseudomonas aeruginosa infection in a burn unit. Journal of Trauma, 63:164-200.
  7. Bach, H.J., Tomanova, J., Scholter, M., and Munch, J.C. (2002). Enumeration of total bacteria and bacteria with genes for proteolysis activity in pure cultures and in environmental samples by quantitative PCR mediated amplification. Journal of Microbiological methods, 49:235-245.
  8. Bahar, M.M., Jangjoo, A., Amouzeshi, A., and Kavianifar, K. (2012). Wound infection incidence in patients with simple and gangrenous or perforated appendicitis. Archives of Iranian Medicine, 13(1): 3-16.
  9. Barie, P. S. (2012). Surgical infections and Antibiotic use In: Courmey M. Townsend, editor. Sabiston Textbook for Surgery: The Biological Basis of Modern Surgical Practice; 19th Ed. New Delhi Elsevier; 240 – 280.
  10. Biadgleyne, F., Abera, B. and Alem, A. (2009). Bacterial isolates from wound infection and their antimicrobial susceptibility pattern in felige Hiwol referral Hospital North-west Ethiopia. Ethiopian Journal of Health Sciences,19: 173 – 177.
  11. Bikowski, J. (1999). Secondarily infected wounds and dermatoses: a diagnosis and treatment guide. Journal of Emerging Surgical Infections, 17: 197-206.
  12. Bowler, P.G., Duerden, B.I. and Armstrong, D.G. (2001). Wound microbiology and associated approaches to wound management. Clinical Microbiology Reviews, 14(2): 244 – 269.
  13. Bowler, P.G., Duerden, B.I. and Armstrong, D.G. (2007). Wound microbiology and associated approaches to wound management. Clinical Microbiology Review, 14(2): 244-269.
  14. Cheesebrough, M. (2006). District laboratory practice in Tropical countries. Cambridge University Press, London, UK pp. 62.
  15. Clinical and Laboratory Standard Institute (CLSI) (2014). Performance standard for antimicrobial susceptibility testing. Twenty- fourth information supplement 34(1) M100- S 241.
  16. Gautam, R., Acharya, A., Nepal, H.P. and Shrestha, S. (2013). Antibiotic susceptibility pattern of bacterial isolates from wound infection in chitwan medical college teaching hospital, chitwan, Nepal. International Journal of Biomedical and Advance Research,4(4): 248 – 252.
  17. Gerald, P. B., Ricardo, B., Victor, F. and Leena, J. (2016). Infections caused by Pseudomonas aeruginosa. Clinical infectious Diseases,5(2): 279 – 313.
  18. Giacometti, A., Cirioni, O., Schimizzi, A.M., Del Prete, M.S., Barchiesi, F., Diacometti, M., Drapeau, C.M.J., Nicastri, E., Martine, C., Ippolito, G., and Moro, M.L. (2000). Surgical site infection in Italian Hospitals: A prospective Multicenter study. Biomedical Complementary and Infectious Disease,8(34):1471 -1480
  19. Houang, E. T. (1991). Antibiotic prophylaxis in hysterectomy and induced abortion. A review of the evidence, Drugs; 41: 19 – 37.
  20. Kamat, A. A., Brancazio, L. and Gibson, M. (2000). Wound infection in gynecologic surgery. Infectious Diseases in Obstetrics and Gynecology,8(5-6): 230 – 234.
  21. Klevens, R. M., Edwards, J.R. and Richards, C. L. (2007).Estimating Health care-associated infections and death in U.S hospitals 2002, Public Health Report, 122(2): 160 – 166.
  22. Kobamatsu, Y., Makinoda, S, and Yamada, T. (1991). Evaluation of cephems on the prophylaxis of pelvic infection after radical hysterectomy. Gynecological and Obstetric Investigation, 32: 102 – 106.
  23. Lee, J., Marvin, D. and Heimbach, R. (1990). Infection Control in burn center, Journal of Burn Care Rehabilitation, 11:575-600.
  24. Liliana, S.P., Jangale, N., Chowdhary, A. and Davel, G.B. (2005). Surgical infection in clean and clean contaminated cases. Indian Journal of Medical Microbiology, 23(4): 249-252.
  25. Lofgren, M. Poromaa, I.S., Sternjadhl, J. H. and Renstrom, B. (2004). Postoperative infections and antibiotic prophylaxis for hysterectomy in Sweden: a study by the Swedish National register for gynecologic surgery. Acta Obstetricia et Gynecologica scand; 83: 1202 - 1207.
  26. Mahy, B.W., Meuten, T. (2007).Topley and Wilsons Microbiology of Microbial infection 10th ed. Bacteriology London Hordder Arnold. P. 163-171.
  27. Mangram, A.J., Horan, T.C. Pearson, M.C. Silver, L.C. and Jarvis, W.R. (1999). Guideline for prevention of surgical site infection. Hospital Infection Control Practices Advisory Committee. Infection Control and Hospital Epidemiology, 20: 250-278.
  28. Merril, R. M. (2013). Hysterectomy surveillance in the United States. 1997 through 2005. Medical Science Monitor 2008: 14: CR 24 – CR 31.
  29. Miranda, C., Alados, J. C. and Molina, J. M. (1993).Post hysterectomy wound infection. A review. Diagnostic microbiology and Infectious Disease, 17:41 – 44.
  30. Nitin, G. I, Nikhil, P., Mahesh, A, Amod Yadau, B. L. Chandharu, A. S. (2013): postoperative wound infection. Bacteriology and antimicrobial sensitivity pattern. Sani, R. A., Garba, S. A. and Oyewole, O. A. (2012). Antibiotic Resistance Profile of Gram negative bacteria isolated from wound in general hospital minna, Bida, Kontagora and suleja Areas of Nigeria State. American Journal of Medical Sciences,2(1): 20 – 24.
  31. Shrestha, B., and Basnet, R. B. (2009). Wound infection and antibiotic sensitivity pattern. Journal of Nepal,20(1): 141-152.
  32. Prakash, K.S. (2010). Antibiotic pattern of acute community infections: a global survey. International Journal of Antimicrobial Agents, 4:20-30.
  33. Prasthisher, P., Rekha, R. and Kumar, F. and Bhavnani, (2017). Aerobic bacterial profile of postoperative wound infections and their antibiotic susceptibility pattern of current international journal of applied medical research, Sciences 9:396 – 411.
  34. Raza, M. and Ranabhat, A. (2013). Antimicrobial Susceptibility patterns of the bacteria isolates from postoperative wound infections in Kathmandu. Nepal Open Journal of Medical Microbiology,3:159 – 163.
  35. Reichman, D.E. and Greenberg, J.A. (2009). Reducing surgical site infections: Review of strategies. Reviewer Obstetrics and Gynecology 2 (4): 212-221.
  36. Rubin, R.H. (2006). Surgical wound infection: epidemiology, pathogenesis, diagnosis and management. Biomed Cerner Infectious Diseases, 6: 171-172.
  37. Zorgani, A; Zaidi, M; Ranka, R. and Shaben, A. (2002). The pattern and outcome of septicemia in a burns intensive care unit. Annual Burns and Fire Disasters, 15:179-182.
How to Cite

U, N. I., K.C, E., C.N, N., & S.O., N. (2020). Susceptibility Pattern of Bacteria Isolated from Postoperative Wounds of Hysterectomy Patients in Catholic Hospitals at Umuahia, Abia State, Nigeria. Nigerian Journal of Microbiology, 34(2), 5343-5350. https://doi.org/10.67614/njm.2020.6mh762yj

N. I. U, E. K.C, N. C.N, and N. S.O., "Susceptibility Pattern of Bacteria Isolated from Postoperative Wounds of Hysterectomy Patients in Catholic Hospitals at Umuahia, Abia State, Nigeria," Nigerian Journal of Microbiology, vol. 34, no. 2, pp. 5343-5350, December 2020. doi: 10.67614/njm.2020.6mh762yj

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