Volume 28 (2015)

1

Research Article

HEPATITIS B AND C SEROPOSITIVITY IN A COHORT OF HIV-POSITIVE PATIENTS IN ILORIN, NORTH-CENTRAL NIGERIA

Udeze A.O^*, Ali U.M, Adeoye P.A, Odugbesi A.E, Sule W.F, Okonko I.O

Since HIV, HBV and HCV share common routes of transmission and acquisition, HIV infected patients are likely exposed to HB and C viruses. We hypothesised that there was no difference between prevalence rate of HB and C infections among HIV-infected patients accessing healthcare at HIV and AIDS section of University of Ilorin Teaching Hospital, Ilorin, Nigeria. This is a hospital-based cross sec...

Aug, 2015 pp. 2767-2776

2

Research Article

In Vitro DIGESTIBILITY EVALUATION OF RICE BRAN MEAL INCORPORATED WITH PRECIPITATE p-D MANNANASE FROM Penicillium italicum

Ekundayo, T. C^*, Olaniyi, O.O

The effect of addition of crude and ammonium sulphate ((NH^SQi) precipitation of P-D-mannanase produced by Penicillium italicum on in vitro dry matter digestibility (IVDMD) and in vitro protein digestibility (IVPD) of rice bran meal intended for animal feed formulation was studied. Firstly, the crude enzyme (non-precipitated), and (NH-1)SO4 precipitated enzyme were incorporated in the series o...

Aug, 2015 pp. 2760-2766

3

Research Article

MICROBIAL EVALUATION OF MANICURE AND PEDICURE SHOPS ALONG ADEWOLE ESTATE, ILORIN KWARA, NIGERIA

Onajobi I. B. ,, Okerentngba P.O.^*, Adeyemi S. A., Laba S. A.

In this study, isolation and characterization of microorganisms associated with trimmed human nails were investigated. The trimmed nails were collected from three Manicure and Pedicure shops along Adewole Estate, Ilorin, Kwara State over a period of nine weeks. Microbial isolation was done using a standard serial dilution technique. The colonial morphology and biochemical study of the isolates...

Jun, 2015 pp. 2939-2945

4

Research Article

MICROBIAL RESPONSE TO PLANTS' ROOTS AND HYDROCARBON CONTAMINANTS IN DIESEL-OIL IMPACTED TROPICAL SOIL

Adieze, Ifechukwu E.^*, Onogbou, C. D., Ihejirika, C. E., Nwabueze, R. N.

Microbial response to plants' roots and contaminants in diesel-oil impacted soils were assessed in a greenhouse study. Two plants, Pueraria spp. and Panicum maximum were planted in 6% w/w polluted and control soil samples to obtain contaminated planted and uncontaminated planted pots. Contaminated control and uncontaminated control pots were also maintained. Periodically, from week zero to 8 ...

Jun, 2015 pp. 2927-2938

5

Research Article

SELECTION OF MEDIA, SUITABLE FOR Azotobactervinelandii BIOSYNTHESIS OF Poly-[3-hydroxybutyrate AND ITS EXTRACTION WITH CHLOROFORM.

Mbachu, I. A. C^*

Petroleum derived plastics constitute environmental problem because they are not biodegradable. Alternative substrates for manufacture of biodegradable plastics are necessary to safeguard the environment. Some bacterial genera produce Poly-p hydroxybutyrate (PHB), under adverse nutrient conditions, which serve as alternatives for petroleum derived plastics. Azotobacterspis associated with its b...

Jun, 2015 pp. 2919-2926

6

Research Article

PROSPECTIVE STUDY ON THE INCIDENCE OF MALARIA PARASITE INFECTION AMONG CHILDREN (0- 15 YEARS AGE) ATTENDING DUTSE GENERAL HOSPITAL, JIGAWA STATE, NIGERIA

Salisu, A^*, Abdulhadi, Y.

The study was aimed at determining the occurrence state of malaria parasite infection among children (0-lyears age) attending Dutse General Hospital Jigawa state, served as sites for a prospective study. A total of two Hundered (200) blood samples were collected from the subjects and examined using standard microscopic method. The result was recorded as positive or negative to malaria parasite...

Jun, 2015 pp. 2915-2918

7

Research Article

PHYTOREMEDIATION AS AN ALTERNATIVE TECHNOLOGY TO CLEANS UP ENVIRONMENTAL CONTAMINATION IN JIGAWA STATE. NIGERIA: A REVIEW

Abdulhadi, Y., Salisu, A^*

Bioremediation is a technology that utilizes the metabolic potential of microorganisms to clean up contaminated environments. The purpose of this review is to assess the current state of. Phytoremediation as an innovative technology and to discuss its usefulness and potential in the remediation of various impacted sites. Since the dawn of industrial revolution and Agricultural activities manki...

Jun, 2015 pp. 2907-2914

8

Research Article

VOLUNTARY COUNSELLING AND TESTING FOR HEPATITIS B VIRUS IN APPARENTLY HEALTHY SUBJECTS OF A NIGER DELTA REGION, NIGERIA

Awujo, N. Chinedu^*, Uwaezuoke J. C., Anyaorah, C. Frances, Ezeonwumelu, J.O. Chukwujekwu

Sera of some individuals (260 males and 230 females) resident at a Niger Delta region of Osisioma Ngwa Local Government Area of Abia State, Nigeria were tested for the presence of antibodies to hepatitis B virus (HBV) using the rapid chromatographic and enzyme-linked immunosorbent assays (ELISA). Out of a total of 490 collected and examined samples, 4.08% tested positive for HBV antibodies. Th...

Jun, 2015 pp. 2902-2906

9

Research Article

ANTIGENIC CROSS REACTION BETWEEN MALARIA PARASITES AND TYPHOID ORGANISMS IN PATIENTS WITH FEVER OF UNKNOWN ORIGIN IN OWERRI MUNICIPAL IMO STATE

Ajero C.M.U^*, Uzoagba, D. C.

This study reports result of malaria parasite test, Widal test and bacteriological test was carried out on 205 samples. Of these, 160 were cases of patients presenting malaria-like symptoms while 45 were healthy controls. Out of the 160 patients studied, 117(73.1%) had acute malaria, while 18(40%) out of the 45 control sample were malaria parasite carriers. Widal test was done to determine tho...

Jun, 2015 pp. 2892-2901

10

Research Article

DISTRIBUTION OF INTESTINAL PARASITES IN FECAL EFFLUENTS FROM CATTLE SLAUGHTERED AT THE ABAKALIKI ABATTOIR

Agumah N. B, Okonkwo E. C, Igwenagu H.^*, Afmkiva F. N., KNivadiogbu I., Ebiega-Oselebe I. N., Ali.

A study to determine the distribution of intestinal parasites in fecal effluents from cattle was carried out at the Gariki abattoir in Abakaliki; Ebonyi State. Samples were collected from cow slurries. A total of 200 samples were examined for intestinal parasites. The conventional wet preparation procedure by the World health organization and the Formol ether concentration technique were used ...

Jun, 2015 pp. 2878-2882

11

Research Article

DIOXYGENASE SCREENING IN INDIGENOUS HYDROCARBON UTILIZING BACTERIA FROM OIL POLLUTED SITES IN NIGER DELTA, NIGERIA

Chioma Blaise Chikere, Kingsley Omadudu^*

Soil and sediment samples collected from crude oil impacted sites at Ogale-Eleme, Rivers State, and five water samples from effluent discharge points at five different flow stations in Delta State, all in the Niger Delta area of Nigeria respectively were analyzed for the presence of hydrocarbon utilizing bacteria using Bushnell-Haas agar, followed by dioxygenase screening. A total of sixty bact...

Jun, 2015 pp. 2883-2891

12

Research Article

ANTIMICROBIAL RESISTANCE IN ENTEROCOCCI ISOLATED FROM POULTRY IN OWERRI, IMO STATE NIGERIA

Iketneh, C. O, Chikwendu, C. L^*, Amadi, E.S

Poultry are increasingly being associated with carriage of multi-resistant organisms that may cause disease in humans. This study evaluated the prevalence and antimicrobial resistance in enterococci isolated from poultry in Imo State Nigeria. One hundred and thirty (130) Enterococcns spp. were isolated from cloacal swabs, fecal and litter samples from different poultry farms. The isolates were...

Jun, 2015 pp. 2860-2871

13

Research Article

ASSESSMENT OF THE MICROBIOLOGICAL STATUS OF SOME COMMERCIAL YOGHURT BRANDS SOLD IN OWERRI METROPOLIS, IMO STATE, NIGERIA

Chikwendu, C. L^*, Obi, R. K, Amadi, E.S

An assessment of the microbiological quality of seven commercial brands of yoghurt drinks sold in Owerri metropolis was determined using standard microbiological procedures. The physical parameters of the brands at time of purchase were also determined. The results showed that the pH values of the samples ranged from 4.29-4.46, while their temperature readings were between 8 and 17°c. While av...

Jun, 2015 pp. 2872-2877

14

Research Article

MICROBIOLOGICAL EVALUATION OF ROASTED CHICKEN SOLD IN KEFFI METROPOLIS

Makut, M.D.^*, Nsemoh, H. E.

Microbial evaluation of roasted chicken sold in Keffi metropolis was investigated. Samples of the roasted chickens were collected in triplicates from seven different sales locations for an assessment of their microbiological qualities. Spread plate technique was employed for the isolation of the microorganisms following serial dilution of the samples. The hands of the vendors preparing and sel...

Jun, 2015 pp. 2838-2843

15

Research Article

THE PRESERVATION OF KUNUN-ZAKI USING CHEMICAL PRESERVATIVES, REFRIGERATION AND PASTEURIZATION

Omojasola P.F.^*, Olabisi Flora Davies

The potential of three preservation techniques: the use of two chemical preservatives; sodium benzoate and sodium metabisulphite, pasteurization and refrigeration for the preservation of kunun-zaki was evaluated. The treated samples were stored for four weeks. Microbiological analyses and assay of physico-chemical parameters such as pH, titratable acidity, sugar content, mineral content and al...

Jun, 2015 pp. 2844-2859

16

Research Article

ISOLATION OF MICROORGANISMS ON THE SURFACE OF MOBILE PHONES

Anibijuwon I.I.^*, Odaibo D. O., Omojasola P.F., Ibrahim, F. R

This study was conducted to determine microbial contamination of mobile phones in the university of Ilorin campus, in the central north region of Nigeria, and identify the most important microbial species associated with these phones in order to take the necessary remedial measures. The analysis of a total of 202 samples was done to identify fungal and pathogenic bacteria isolates. Sterile swa...

Jun, 2015 pp. 2821-2828

17

Research Article

MICROBIOLOGICAL ASSESSMENT OF JEW'S EAR MUSHROOM (AURICULARIA AURICULAR-JUDAE) SOLD IN PORT HARCOURT, RIVERS STATE.

Odu, N. N., Stanley, H.O.^*, Didia, A

Microbiological assessment of Auricularia auricular-judae sold in three markets in Port Harcourt metropolis was carried out. Nine microbial genera were isolated and identified on the basis of their microbiological, morphological, biochemical and physiological characteristics. The heterotrophic bacterial counts ranged between 1.8 X 104 - 4.7 X 105 Cfu/g whereas the heterotrophic fungal counts r...

Jun, 2015 pp. 2828-2832

18

Research Article

EVALUATION OF DISINFECTANTS BY THE IN-USE TECHNIQUE IN HEALTHCARE FACILITIES IN KOGI STATE NORTH CENTRAL NIGERIA

Akabueze E.C, Obi SC, Nwankivo E.O^*

The aim of this research was to evaluate the efficacy of disinfectants by in-use testing and to identify bacterial isolates contaminating disinfectants in different hospitals. Ninety two samples of disinfectants comprising Purit, Izal, Bleach, Spirit and Dettol being used in twenty hospitals in Kogi State were analyzed for efficacy between July and December 2011 by in-use technique. Samples of...

Jun, 2015 pp. 2833-2837

19

Research Article

ANTIBIOGRAM PATTERN OF Staphylococcus Saprophytics ASSOCIATED WITH ASYMPTOMATIC BACTERIURIA AMONG PREGNANT WOMEN ATTENDING ANTENATAL CLINICS IN BAUCHI METROPOLIS

Iliyasu, M.Y.^*, Ezihe, M.I, Bala, K.

A study to isolate and determine the antibiogram pattern of Staphylococcus saprophytics associated with asymptomatic bacteriuria in pregnant women was carried out. A total of ninety one (91) clinical urine samples were collected from antenatal women attending some hospitals in Bauchi metropolis. Out of these samples 32.9% (30 of 91) were positive with S. saprophytics for significant bacteriuri...

Jun, 2015 pp. 2812-2820

20

Research Article

EFFICACY OF WHEAT STRAW AND COTTON WASTE AS SUBSTRATES IN THE PRODUCTION OF EDIBLE MUSHROOMS

Musa, H.^*, Wuyep, P., Ali, B.D

Wheat straw and cotton waste were used as substrates and some natural additives; nitrogen and carbon sources were used as nutrient supplements to enhance mycelia growth of Pleurotusostreatus (Florida). Sundried wheat straw substrate was cut into small pieces. The minced wheat straw and cotton waste substrates were soaked in boiled water and excess water squeezed out using a clean muslin cloth....

Jun, 2015 pp. 2804-2811

21

Research Article

BACTERIOLOGICAL ASSESSMENT OF TREATED PIPED WATER IN PARTS OF ILORIN METROPOLIS

Eniola, K.I.T^*, 0daibo, A. D, Olayemi A. B, Ajiboye, T.O

Abstract The bacteriological quality of treated piped water in distribution system in Ilorin metropolis was assessed. The pH, suspended solid and microbiological characteristics (heterotrophic bacteria count and coliform count) of treated water collected from thirty different locations within the metropolis were determined. The samples were slightly acidic (pH 5.16 to 6.53); the suspended solid...

Jun, 2015 pp. 2797-2803

22

Research Article

BACTERIOLOGICAL QUALITY OF POTABLE WATER FROM DIFFERENT SOURCES IN KEFFI, NASARAWA STATE, NIGERIA

Makut, M.D.^*, Kelechi, A, Alabi, S.

An investigation was carried out to determine the bacteriological quality of potable water from different locations and sources within Keffi metropolis. Water samples from Tap, Borehole and Well were collected in duplicates from ten (10) different locations within Keffi metropolis. Samples obtained were analyzed bacteriologically using standard methods. The spread plate method following serial...

Jun, 2015 pp. 2791-2796

23

Research Article

ASSESSING THE SANITARY AND MICROBIAL RISK ASSOCIATED WITH HAND-DUG WELLS

Dick, Asiton-A Asifamabia1^*, Abu, Gideon O

The sanitary and microbial risk assessment of the hand-dug wells in Oproama Community was undertaken between February 2010 and January 2011. The sanitary risk assessment of the hand-dug wells reveals very high risk (8-10) based on physical protection of the water point; distance to sources of contamination and open defecation. The quantitative microbial risk assessment (QMRA) of the hand-dug w...

Jun, 2015 pp. 2777-2790