Research Article

Phytochemical Screening and Antibacterial Efficacy of Apiary Honey on some bacteria isolated from Diabetic Foot Ulcer

Unegbu Nnachetam Valentine1*, Nwachukwu Obiora Ndubuisi2, Obum-Nnadi Charity Ndidi3, Nkwoemeka Ndidi Ethel4, Emmanuel Nnabuike Ugbo5, Egwuatu Pius Ikenna1
1 Department of Microbiology, Renaissance University, Ugbawka, Enugu State, Nigeria
2 Department of Microbiology, Abia State University, Uturu, Abia State, Nigeria
3 Department of Medical Microbiology, Nnamdi Azikiwe University, Awka, Anambra State, Nigeria
4 School of General Studies/Microbiology Department, University of Nigeria, Nsukka, Enugu State
5 Department of Applied Microbiology, Ebonyi State University, Abakaliki, Nigeria
* Corresponding author: donval4u@yahoo.com
Published: Jun, 2019
DOI: 10.67614/njm.2019.wzih4czj
Pages: 4551-4558

Abstract

Diabetic wounds unlike typical wounds are slower to heal, making treatment with conventional topical medications an uphill process. Among several different alternative therapies, honey is an effective choice because it provides comparatively rapid wound healing. The apiary honey samples used in this study were purchased from Umudike research Institute Umuahia, in Abia State, Southeastern Nigeria. Phytochemical screening, antibacterial susceptibility test, minimum inhibitory concentration and minimum bactericidal concentration were performed using standard methods. Phytochemical analysis revealed the presence of reducing sugar, saponins, glycosides, alkaloids and flavonoids and absence of Terpenoids, Phlobatanins, phenols and tannins. Antibacterial activity of apiary honey on some medically important bacteria including Staphylococcus aureus, Escherichia coli, Klebsiella spp, Bacillus subtilis, Proteus vulgaris and Pseudomonas aeruginosa shows that apiary honey exhibit strong antibacterial activity producing zones of inhibition against the tested bacteria. Also, honey sample used in this study showed antibacterial activity than the commercially available antibiotics, both of which were of the same concentration of 2.5ml. The results from this study shows that apiary honey, apart from its roles as food and supplements, could be used as an antibacterial agent, as they may be an excellent alternative to curtail the further spreading of drug resistant bacteria in Nigeria.

Keywords

Antibacterial susceptibility Diabetic wounds Apiary Honey Phytochemical screening.

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How to Cite

Valentine, U. N., Ndubuisi, N. O., Ndidi, O. C., Ethel, N. N., Ugbo, E. N., & Ikenna, E. P. (2019). Phytochemical Screening and Antibacterial Efficacy of Apiary Honey on some bacteria isolated from Diabetic Foot Ulcer. Nigerian Journal of Microbiology, 33(1), 4551-4558. https://doi.org/10.67614/njm.2019.wzih4czj

U. N. Valentine, N. O. Ndubuisi, O. C. Ndidi, N. N. Ethel, E. N. Ugbo, and E. P. Ikenna, "Phytochemical Screening and Antibacterial Efficacy of Apiary Honey on some bacteria isolated from Diabetic Foot Ulcer," Nigerian Journal of Microbiology, vol. 33, no. 1, pp. 4551-4558, June 2019. doi: 10.67614/njm.2019.wzih4czj

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