Research Article

Phytochemicals Screening and Antibacterial activity of Ethanolic leaf extracts of Terminalia catappa on some clinical bacterial isolates

Gurama, A. G1*, Shamsuddeen, U.2, Dahiru, A. T3
1 Department of Microbiology, Bayero University Kano
2 1Department of Microbiology Faculty of Life Science Bayero University Kano PMB: 3011
3 Department of Hospitality Management, School of Technology, Kano State Polytechnic
* Corresponding author: gochop20@gmail.com
Published: Jun, 2019
DOI: 10.67614/njm.2019.wv61lye6
Pages: 4539-4544

Abstract

This study is aimed at evaluating the antimicrobial activity of ethanolic leaves extract of Terminalia catappa on some clinical bacterial isolates. The extracts were tested in vitro on Gram negative bacteria, it was obtained from ethanol using percolation method. The sensitivity of four clinical isolates of Escherichia coli, Proteus mirabilis, Morganella morganiis, Yersina enterocolitic was tested. The extract demonstrated strong in vitro antibacterial activity against these organisms at all concentration used (20μg/disc, 40μg/disc, 80μg/disc and 160μg/disc). Of these bacteria screened for antibacterial activity Morganella morganiis appeared to be the most sensitive organism exhibiting growth inhibition zone diameter of 15mm (160μg/disc), 14mm (80μg/disc), 12mm (40μg/disc), and 9mm (20μg/disc). While the least sensitive organism was observed on Escherichia coli 14mm (160μg/disc), 11mm (80μg/disc), 10mm (40μg/disc), 9mm (20μg/disc). Minimum Inhibitory concentration (MIC) of the extract was determined at 25ug/ml for the entire test organism. Although the extract revealed a strong inhibitory activity against the test organisms, growth was observed when test-tubes which showed absence of growth at MIC were sub-cultured on solid media to determine Minimum Bacterial Concentration (MBC) meaning that the extract was only Bacteriostatic at these concentration.

Keywords

E. coli Terminalia catappa Morganella morganiis Yersina enterocolitic MBC MIC.

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How to Cite

G, G. A., U., S., & T, D. A. (2019). Phytochemicals Screening and Antibacterial activity of Ethanolic leaf extracts of Terminalia catappa on some clinical bacterial isolates. Nigerian Journal of Microbiology, 33(1), 4539-4544. https://doi.org/10.67614/njm.2019.wv61lye6

G. A. G, S. U., and D. A. T, "Phytochemicals Screening and Antibacterial activity of Ethanolic leaf extracts of Terminalia catappa on some clinical bacterial isolates," Nigerian Journal of Microbiology, vol. 33, no. 1, pp. 4539-4544, June 2019. doi: 10.67614/njm.2019.wv61lye6

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