Volume 39 (2025)

1

Research Article

Evaluation of Bacteriocins against Multi-drug Resistant Bacteria Implicated in Urinary Tract Infections

Ikott I. U.^*, Antia U. E., Bassey M. E., Daniel O. E.

As the economic burden of treating urinary tract infections (UTIs) is gravely increasing due to multi-drug resistance (MDR) among uro-pathogens, the search for new sources of antimicrobial compounds including bacteriocins has been a priority recently. This study therefore, evaluated the antibacterial activity of bacteriocins against multi-drug resistant bacteria implicated in UTIs. Fermented food ...

Dec, 2025 pp. 7719 - 7730

2

Research Article

Comparative In Vitro Antifungal Activities of Zingiber officinale, Justicia carnea, Hibiscus sabdariffa Nanosynthesized Extracts against Uropathogenic Candida albicans

Oladunmoye M. K^*, Idu M., Amoo I. A, Oladejo B. O., Bodunrinde R. E, Akinterinwa E. T.

Abstract: Urogenital infections caused by Candida species (candiduria) are a growing public health concern, exacerbated by the rise of antifungal-resistant strains. This necessitates the search for novel, effective antifungal agents, with medicinal plants being a promising source. Ethanol, chloroform, and hot water (aqueous) extracts of Zingiber officinale (rhizome), Justicia carnea (leaf), and Hi...

Dec, 2025 pp. 7731 - 7740

3

Research Article

Resistance pattern of Mycobacterium tuberculosis Complex Isolated from Re-treatment Patients to First Line Anti-Tuberculosis Drugs in Zaria Nigeria

Mamuda K^*, Ahmadu I., Aliyu, M.S, Suleman, M, Ibrahim A. K, Song M. A, Garba B. Y, Aminu D. A, Dan-Inna M. Z., Mathew G

Tuberculosis (TB) is one of the leading infections that cause death worldwide. The emergence of drug-resistant Mycobacterium tuberculosis is of great concern for National TB control programmes wich hinders the control of tuberculosis; therefore it has become important to screen infected patients for resistant TB. A total of 437 sputum samples were collected from re-treatment TB patients. The sampl...

Dec, 2025 pp. 7741 - 7747

4

Research Article

Assessment of the Level of Cytokines in People living with HIV, HCV and HIV/HCV Individuals in Port Harcourt Metropolis, Rivers State, Nigeria

Glory Barinuaka Baeka^*, Oloke J. K

Cytokines, play a crucial role regulating the immune response and coordinating the communication between cells of the immune system and other tissues. The co-infection of HIV/HCV in patients compared to mono infections has been discovered to have reduced anti-inflammatory cytokines in peripheral blood mononuclear cells (PBMC) and dehydroepiandrosteronesulphate in plasma. The aim of this study ther...

Dec, 2025 pp. 7701 - 7708

5

Research Article

Seroprevalence of COVID-19 and Its Associated Factors Among Nigerian University Students: A Cross-Sectional Study

Kelly Elimian^*, Afamefuna Dunkwu-Okafor

Universities were potential hubs for SARS-CoV-2 transmission in Nigeria; however, empirical data on the infection burden among students have been limited. The study assessed the seroprevalence of SARS‑CoV‑2 and associated factors among undergraduates at a public University to inform educational and public health policy. The study used a cross-sectional design and was conducted among systematically...

Dec, 2025 pp. 7709 - 7718

6

Research Article

Antioxidant, Functional and Sensory Properties of Wine Produced from Yellow Mombin (Spondias mombin L.) Using a Wild Yeast (Saccharomyces cerevisiae) from Palm Wine

Osaro-Matthew, R. C^*, Itaman, V.O, Oti V. C

Yellow mombin is an underutilized indigenous fruit characterized by unique flavor and high sugar content making it suitable for exotic wine production though fermentation. This study was aimed at exploiting the fermentative capability of palm wine Saccharomyces cerevisiae in the production of wine and evaluating the functional, antioxidant and sensory attributes of wine produced from yellow mombin...

Dec, 2025 pp. 7688 - 7700

7

Research Article

Microbiological, Parasitological and Heavy Metals Assessment of selected Vegetables in Offa Metropolis

Olawore T. R^*, Said R. O, Awe, Sunday

Vegetables are vital dietary components, supplying essential nutrients for human health. However, their safety can be compromised by various contaminants. This study assessed the microbiological, parasitological, and heavy metal contamination of ten commonly consumed vegetables— Telfairiaoccidentalis, Amaranthus spp., Celosia argentea, Corchorusolitorius, Vernonia amygdalina, Solanumlycopersicum, ...

Dec, 2025 pp. 7677 - 7687

8

Research Article

Incidence and Antibiotic Susceptibility Profile of Plesiomonas shigelloides Isolated from Fish, Fish Storage Water and Fish Seller’s Towel

Temitope Deborah Agboola^*, Oguntimehin M. O, Obadofin I. O, Oyeneye O. R.

Plesiomonas shigelloides is an emerging pathogen commonly found in aquatic environment that had been implicated in diverse gastrointestinal diseases. This study evaluated the incidence of Plesiomonas shigelloides and their antibiogram pattern in fish storage water, fish and fish seller’s towel swab. Samples were collected from two major markets in Southern part of Ondo State, Nigeria and broth cul...

Dec, 2025 pp. 7656 - 7668

9

Research Article

Microbial Quality and Public Health Risk Assessment of Ready-to-Eat Suya Meat in Lagos, Nigeria: Prevalence of Pathogenic Bacteria and Implications for Food Safety

Oladeinde Olatunde Ogundipe^*, Folounso T. V, Abisoye F. S, Adefehinti O. J, Teniola O. D

Suya, a popular Nigerian skewered meat (pH 5.7–7.2; aw 0.98–0.99), is a low-acid, high- moisture food that is highly susceptible to microbial contamination. Pathogens such as Escherichia coli, Salmonella spp., and Staphylococcus aureus pose severe public health risks in developing regions, where contaminated meat often serves as a primary transmission pathway. These risks are exacerbated by inadeq...

Dec, 2025 pp. 7669 - 7676

10

Research Article

Bacteriological and Physicochemical Assessment of Digestates after Anaerobic Digestion

Oyem I. M, Amrasa O. S, Ajieh M. U, Oshoma C. E^*

The demand for an alternative to inorganic fertilizer is steadily increasing due to the drawbacks associated with its usage. The utilization of digestates has gained significant recognition recently due to their remarkable benefits even after being applied to the soil. Hence, this investigation aimed to assess the bacteriological profile of digestates, effluents, and feedstock originating from var...

Dec, 2025 pp. 7645 - 7655

11

Research Article

Microbial and Physicochemical Evaluation of a Communal Piggery Wastewater and Its Bioremediation Using Autochthonous Bacterial Isolates

Olubukola Olayemi Olusola-Makinde^*

Piggery activities necessitate large amount of wastewater generation with high concentrations of organic matter and pathogens. This study characterized the microbial and physicochemical properties of discharged piggery wastewater and its bioremediation potential. Wastewater samples collected weekly from May to July, 2024 at piggery farm, Federal University of Technology Akure, were subjected to mi...

Dec, 2025 pp. 7634 - 7644

12

Research Article

Keratinophilic Fungi Isolated from Soil of School Playgrounds in Abakpa Nike Town, Enugu State, Nigeria

Olisaka F. N^*, Ohakwe R. O, Oyeka, Christie A

In nature, keratinous substrates are broken down by keratinophilic fungus and a major habitat for this fungus is the soil. Their ability to breakdown keratin contributes to the pathogenicity potential of many of these fungi. Their actions may lead to worsen of respiratory diseases and development of a condition known as dermatomycosis, in both humans and animals. This study was aimed at isolating ...

Dec, 2025 pp. 7626 - 7633

13

Research Article

Culture and Molecular Profiling of Microorganisms Associated with Crops Grown on Abattoir Wastewater-Irrigated Soil in Agbor, Delta State, Nigeria

Oghonim P. AN^*, Onuoha, S .C

Untreated abattoir wastewater contains high microbial loads that may persist in irrigated soils and transferred to edible crops. This study investigated the culture and molecular profiles of microorganisms isolated from abattoir wastewater, it’s irrigated soils and crops cultivated on the receiving soils with particular emphasis on soil and crop-associated microbiomes in Agbor, Delta State, Nigeri...

Dec, 2025 pp. 7616 - 7625

14

Research Article

Isolation and Identification of Bacterial and Fungal Isolates from Door Handles in University of Benin Male and Female Post-Graduate Hostels

Ikponmwosa I. J^*, Igiehon O. N, Akinnibosun F. I.

The hands are the chief organs for physical manipulation of the environment. They serve as a medium for the propagation of microorganisms from place to place and from person to person. Human hands usually harbour microorganisms both as part of the body’s normal flora as well as transient microorganisms contracted from the environment. This study was aimed at isolating and identifying bacterial and...

Dec, 2025 pp. 7606 - 7615

15

Research Article

Assessment of Microbial and Physicochemical Characteristics of Groundwater in Selected Local Government Areas of Oyo State, Southwest Nigeria

Gbadebo A. M^*, Ishola S. A, Gbadebo O. O, Adebambo O. A

Negative impacts of contaminated groundwater on human health are chronic and are very difficult to detect. This study assessed the extents of groundwater contaminations based on the level of microbial and physicochemical characteristics in selected local government areas (LGAs) of Oyo-State, Southwestern Nigeria. Groundwater samples were collected from 10 wells per each LGA (50 wells in total), in...

Dec, 2025 pp. 7580 - 7605

16

Research Article

Undergraduate Microbiology Research at the University of Benin, Nigeria: Experiences, Challenges, and Strategic Opportunities

Kelly Elimian^*, Ajuzie C. U

Undergraduate research is a core component of Microbiology training and a crucial pathway for enhancing scientific capacity. Despite its recognised value, students in resource-limited settings often complete research projects under substantial infrastructural and institutional constraints. This study assessed the research experiences of final-year Microbiology undergraduates at a University in Sou...

Dec, 2025 pp. 7571 - 7579

17

Research Article

Characterization and Stability of Biosurfactant Produced by Xanthomonas campestris Isolated from Tannery Effluent

Usman Ali Bukar^*, Kawo, A. H., Yahaya, S, Inuwa A. B, Fardami A. Y, Ismail H. Y, Sani I. M

The structural characterization of biosurfactants produced by Xanthomonas campestris isolated from tannery effluents was performed using Fourier Transform Infrared Spectroscopy (FT-IR) and Gas Chromatography–Mass Spectrometry (GC–MS). FT-IR analysis revealed distinct functional groups consistent with amphiphilic biosurfactants. A broad band at 3339 cm⁻¹ indicated O–H stretching associated with hyd...

Dec, 2025 pp. 7564 - 7570

18

Research Article

Mangrove Ecosystem and Crude oil: Reactions of Mangrove Ecosystem to Acute and Chronic Crude oil Contamination

Aina, O.R^*, Olaleye, O.N.

Oil spillages in mangrove ecosystems can be problematic hence, the need to source for bioremediation strategies from within the ecosystem. Red mangrove seedlings were carefully uprooted from mangrove soil of Ugbokodo community of Uvwie Local Government Area of Delta State, Nigeria. These acclimatized seedlings were exposed to acute treatment of one-time application of 150 ml crude oil and chronic ...

Dec, 2025 pp. 7550 - 7563

19

Research Article

Green Synthesis, Characterization and Antimicrobial Effect of Silver Nanoparticles Using Alchornea laxiflora and Spondia mombin Against Microorganisms from Wound and Gastrointestinal Tracts Infections

Thonda O. A^*, Daramola O. O, Aladejana O. M., Wilkie E. D, Olowookere B. D., Olopade E. O., Amodu S, Fagbemi K. O., Oyaniyi A. A.

Silver nanoparticles play a huge roles in creating new anti-microbial properties to tackle a quite number of disease-causing microbes. This study aimed at determining the in vitro anti-microbial properties of silver-nanoparticles (AgNPs) green synthesis on multidrug resistance organisms from Nigerian Institute of Medical Research, Lagos State and University college Hospital, Ibadan. The AgNPs was ...

Jun, 2025 pp. 7539 - 7549

20

Research Article

Serological and Molecular Detection of Hepatitis A Virus among Women of Childbearing Age Attending Some Health Facilities within Maiduguri Metropolis, Borno State, Nigeria.

Papka I. M^*, Bukbuk D. N, Ladan J, Babagana B

Hepatitis A virus (HAV) is the primary cause of acute viral hepatitis globally. It is primarily spread by eating or drinking contaminated food or water, as well as by direct contact (either through sexually or through exchange blood transfusion) with individuals who have been infected. The virus is widespread in low-income nations with unsanitary and poor sociodemographic conditions. In this st...

Jun, 2025 pp. 7528 - 7538

21

Research Article

A Study of Malaria Infection Suppression by Bioactive Compounds from Extracts of a Tropical Medicinal Plant

Chidiebere Agha Otuu^*, Udeh E. O, Eke S. S, Usman-Yamman H, Ekuma I. C, Nwosu C. G, Otuu Q. A, Obiezue N. N

The properties exhibited by natural products from plants, including the medicinal activity, can be ascribed to the type and nature of the biologically active compounds they contain. The scientific study of these bioactive compounds is a very important step in the research to discover new molecules for potential drugs development from such natural products. This study was carried out with the ai...

Jun, 2025 pp. 7521 - 7527

22

Research Article

Phytochemical Screening and Antibacterial Properties of Ethanol and Aqueous Extracts of Allium sativum (Garlic) on Staphylococcus aureus (ATCC 9144), Pseudomonas aeruginosa (ATCC 27853) and Klebsiella pneumoniae (ATCC BAA 1705)

Mohammed S. B^*, Idirisu J. L., Ojodale P. I, Makolo D., Olajugbagbe T. E, Yusuf S. S, Waseve V. K, Chidiebere G. N, Shehu S

The misuse of antibiotics has led to antibiotic resistance which is a major challenge in controlling of infectious diseases despite efforts towards discovery and production of new antibiotics. The search for novel antimicrobial agents to combat pathogens has become crucial. This study aimed at investigating antibacterial activity of ethanol and aqueous extracts of garlic (Allium sativum) against t...

Jun, 2025 pp. 7423-7432

23

Research Article

In-Vitro Biofilm Formation and Antimicrobial Resistance of Metallo Beta-lactamase Producing Pseudomonas aeruginosa of Clinical Origin

Nwankwo F. M.^*, Iroha I. R, Awoke O. A, Ugbo E. N, Oke B

Pseudomonas aeruginosa is an important cause of morbidity and mortality in hospitalized patients and patients with underlying medical conditions. The prevalence of biofilm formation and muti drug resistant strains of P. aeruginosa isolates has been on the increasing. This study was aimed at in-vitro biofilm formation in metallo beta-lactamase producing Pseudomonas aeruginosa of clinical origin. ...

Jun, 2025 pp. 7433 - 7440

24

Research Article

Plasmodium falciparum Multidrug Resistant Gene (Pfmdr-1) Prevalence among Patients Attending Selected Hospitals in Kaduna North and South Local Government Areas, Kaduna State, Nigeria

Dikwa K. B.^*, Musa, H., Musa H, Onwumere G. B, Abdulsalami, M.S, Muhammed M

Malaria is one of the most common causes of morbidity and mortality, Plasmodium falciparum is the most pathogenic species responsible for most malaria cases in Nigeria. Increased prevalence occurs due to evolution of new genotypes resulting in resistance to most common antimalarial drugs used. In malaria endemic regions, drug resistance is a serious public health issue and a major constraint to...

Jun, 2025 pp. 7410 - 7422

25

Research Article

Postharvest Control of Black Rot on African Star Apple (Chrysophyllum albidum L.) Fruits Following Treatment with Hot Water

Oladele O. O.^*, Oyedokun F. D, Ola-Salawu B. B, Ogunsakin O. E., Aderemi O. G.

Black rot is one of the most destructive diseases of African star apple fruits, caused by Aspergillus niger. This study aimed to explore the potential of using hot water (HW) in combating black rot on African star apple fruits. After harvest, the fruits were sorted and selected for maturity and uniform homogeneity and thereafter washed, disinfected in 10% NaOCl for 10 minutes and allowed to air...

Jun, 2025 pp. 7403 - 7409

26

Research Article

Screening and Characterisation of Yeast Species for Citric Acid Production Using Glycerol and Agro-waste

Afolabi Folake Titilayo^*, Adeoye A. E.

The present study was aimed to isolate and screen yeast species for the production of citric acid using glycerol and agro-waste. Fruit samples (orange, lime, lemon and pineapple) were collected from Bodija market in Ibadan. The samples were subjected to microbiological and physicochemical analyses. A total of 43 yeast isolates were recovered from the fruit and 17 isolates had the potential to ...

Jun, 2025 pp. 7390 - 7402

27

Research Article

Significance of “GRAS” Fungi in the Fermentation and Enrichment of Cassava Peels for the Production of Low Cost Feeds for Poultry and Domestic Animals

Adamu J. E.^*, Adiele G. C, Ilesanmi I. A., Ofobuike P. O., Ajewole, O. J., Abiodun O. D., Owolabi S. A., Oduniyi M. A

This study used solid-state fermentation methods to nutritionally enhance cassava peel using a strain of Aspergillus niger, Trichoderma viride and Saccharomyces cerevisiae. Cassava peel were collected, dried and made into crunches. This was fermented for 14 days at room temperature and oven dried. Fermented and unfermented crisp of the peels was analysed for crude protein, fat, fibre, dried mat...

Jun, 2025 pp. 7380 - 7389

28

Research Article

Amylase-Producing Fungi from Cassava Peels: Isolation, Morphological Identification, and Hydrolytic Capability

Abdulmumini S. A^*, Yusuf-Salihu, B.O, Awe, Sunday

Amylases are enzymes of considerable economic importance, extensively employed in the food, fermentation, textile, and biofuel sectors. Utilizing agro-industrial leftovers, like cassava peels, as substrates for enzyme-producing fungi is a sustainable and economical method for discovering microbial biocatalysts. This research sought to extract and evaluate fungal strains from degraded cassava pe...

Jun, 2025 pp. 7374 - 7379

29

Research Article

Assessment of Fishpond Sludge in Bioremediation of Mechanic Workshop Polluted Soil

Stephen E^*, Sau A, Jonathan F, Aminu S, Abubakar S

The use of fish pond sludge in bioremediation of mechanic workshop polluted soil was studied for a period of eight weeks. Soil samples were collected from mechanic village along Mayo Dasa in Jalingo, and transported to Biological Science laboratory Taraba State University, Jalingo. Fifteen kilogram (15 Kg) of the mechanic workshop soil was weighed into four different plastic perforated bowls l...

Jun, 2025 pp. 7367 - 7373

30

Research Article

Bacteriological Quality of Periwinkles (Littorina littorea) and Clams (Mercenaria mercenaria) and Antibiotic Sensitivity Pattern of Isolated Bacteria

Olalemi, A. O^*, Badmus A. O., Baiyere O. L., Arotupin Daniel Juwon

Periwinkles (Littorina littorea) and clams (Mercenaria mercenaria) are vital components of aquatic ecosystems, each harbouring diverse bacterial communities including potential pathogens. This study investigated the bacteriological composition and antibiotic sensitivity profiles of periwinkles and clams sampled from different markets in Ondo and Lagos States, Nigeria. The load and array of bact...

Jun, 2025 pp. 7358 - 7366

31

Research Article

Ecofriendly and Cost Effective Carbon Dot and Microbial Culture in Remediation of Petroleum Hydrocarbon Spill on Soil

Okorhi-Damisa F. B^*, Ogeleka D. F

Bioremediation technology plays a crucial role in ensuring ecological security when addressing petroleum hydrocarbon contamination in the environment. Its advantages include cost-effectiveness, high efficiency, minimal environmental impact, and the absence of secondary pollutants. Recently, carbon dots (CDs) have gained attention due to their exceptional properties, including quantum size, stro...

Jun, 2025 pp. 7346 - 7357

32

Research Article

GC-MS Profiles of Spent Engine Oil Biodegradation: Influence of Bacillus Species Isolated from Oil Contaminated Soil of Auto Mechanic Workshop

Ikuesan F. A^*, Okonji H. I, Adebayo A. J

Spent engine oil, a petroleum hydrocarbon, is often indiscriminately released into the environment thereby posing significant risks to both ecosystems and human health. This study evaluated the biodegradation potential of Bacillus species isolated from oil-contaminated soil. Soil samples were aseptically collected from an auto-mechanic workshop in Okitipupa, Nigeria, and analyzed using standard...

Jun, 2025 pp. 7335 - 7345

33

Research Article

A Preliminary Quality Assessment of Water from Vulnerable Wells in Ago-Iwoye, Part of South-Western Nigeria

Adebisi N. O.^*, Egberongbe H. O, Adekola H. A, Bamidele J. O., Olufemi S. T., Oyesanya O. A., Adewale O. A.

The Ago-Iwoye community is dominated by shallow wells that are prone to the various sources of pollution. This calls for the need to evaluate the groundwater quality using its integrated basic physicochemical feature and the microbial content. Twenty-five (25) samples were collected in a week from different wells at various locations in the studied rural community. This was done in accordance ...

Jun, 2025 pp. 7316 - 7321

34

Research Article

Bacteriological Assessment of some Fish Ponds in Edo State, Nigeria

Idemudia I. B.^*, Okodugha O. L., Ekhaise F. O.

Fish cultivation in a controlled environment has been found to be influenced by microbial contamination. This study was aimed at assessing the bacteriological qualities of three types of fish ponds in the three Senatorial districts in Edo State, Nigeria. The study was carried out between January, 2017 and December, 2017. The bacteriological analyses were carried out using standard microbiologic...

Jun, 2025 pp. 7322 - 7334

35

Research Article

Antibiogram Profile of Pseudomonas aeruginosa from a Cattle Farm Located in Epe Lagos State, Nigeria

Amarachukwu Obayiuwana^*, Akaa T. G, Nwose A. M, Kanife U. C

Antibiotic resistant bacteria (ARB) may be transmitted from livestock to humans through the oral-faecal route. Pseudomonas aeruginosa has been reported to have intrinsic resistance to many antibiotics combined with its ability to easily acquire antibiotic resistance determinants, which poses a significant threat to public health. This study investigated the presence of Pseudomonas aeruginosa, a...

Jun, 2025 pp. 7441 - 7448

36

Research Article

Comparative Assessment of the Efficacy of Selected Antibiotics against Bacterial Isolates from Wound and Urinary Tract Infections

Ilang D. C^*, Onu E. N, Umahi N. E, Orji C. E

This study conducted a comparative assessment of the efficacy of selected antibiotics against bacterial isolates from wound and urinary tract infections from Alex-Ekwueme Federal Teaching Hospital in Abakaliki, Ebonyi State, Nigeria using antimicrobial disc diffusion method. Bacteria were isolated from wound and urine samples, the isolates were identified through biochemical tests and were all ...

Jun, 2025 pp. 7449 - 7507

37

Research Article

Antimicrobial Evaluation of Selected Antibacterial Handwash Brands Marketed in Nigeria against Multiple Antibiotic Resistant Palmar Bacterial Flora of Students in a Tertiary Institution

Osungunna M. O^*, Musa O. R, Akinwumi F. O, Onifade D. I

Hands remain a potent medium of transmission of infectious diseases, while hand hygiene using handwash remains an effective tool for the prevention of this transmission. This study aimed at investigating the susceptibility profiles of multiple antibiotic resistant bacterial isolates associated with the palms of students of Obafemi Awolowo University, Ile-Ife to selected anti-bacterial handwashe...

Jun, 2025 pp. 7508 - 7520