Volume 31, Issue 2 (December)

1

Research Article

Microbial Assessment of Herbal Products in Ota And Its Environs

Alao, Felix Oluwasegun^*, Nejo, Victoria Modupe

This study was carried out to assess the microbial quality of herbal medicinal products being sold in Ota and its environs. A total of 24 bacterial isolates were identified from 32 herbal products purchased from 8 sellers in different parts of Ota. The total plate count (TPC) for bacteria in the herbal samples analysed ranged from 1.1 x 10 to 9.3 x 1OCFU/ml; while the total fungal count ranged ...

Dec, 2017 pp. 3914-3919

2

Research Article

Effect of Microgravity on Most Frequently Isolated Microorganisms from Cosmetics

Arotupin, D. J^*, Adegoke, T. V, Awojobi, K. O

Microorganisms associated with commonly used cosmetics and effects of microgravity on most frequently isolated microorganism were investigated. The microorganisms isolated from the cosmetics were Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, Proteus mirabilis, Bacillus cereus, Proteus vulgaris, Bacillus subtilis, Trichoderma piluliferum and Neocosmospora vasinfecta. Fifty per...

Dec, 2017 pp. 3920-3925

3

Research Article

Characterization and Identification of Antibiotic-resistant Acinetobacter baumannii from Hospital Wastewaters

Etinosa O. Igbinosa^*, Abeni Beshiru

The present study was designed to characterize antibiotic-resistant Acinetobacter baumannii from some selected hospital environments in Benin City, Nigeria. A total of sixty (60) wastewater samples (15 samples from each hospital) were collected from hospital drains in University of Benin Teaching Hospital (UBTH); Health Centre (University of Benin); Central Hospital Benin and Faith Mediplex, Be...

Dec, 2017 pp. 3926-3932

4

Research Article

Fabrication of a Continuous Flow System for Biofilm Studies

Mohammed Jibrin Ndejiko^*, Wan Rosmiza Txina Wan Dagang

Modern and current models such as flow cell technology which enhances a non-destructive growth and inspection of the sessile microbial communities revealed a great understanding of biofilms. A continuous flow system was designed to evaluate possibility of biofilm formation by Escherichia coli DH5a on the stainless steel (type 304) under continuous nutrient supply. The result of the colony iormi...

Dec, 2017 pp. 3933-3941

5

Research Article

Phytochemical Analysis and Antibiofilm Activity of Allium Sativum Against Food-Borne Microorganisms

Ohaegbu C. G^*, Achi N.K., Asamudo N. V

This work evaluates the chemical composition and antibiofilm potential of extracts from Allium sativum in the search for green and effective alternatives for overcoming menace of biofilms. The work evaluated the antimicrobial activity of the extracts against Staphylococcus aureus and Pseudomonas aeruginosa, minimum biofilm inhibitory concentration, inhibition of initial cell attachment as well ...

Dec, 2017 pp. 3942-3952

6

Research Article

Antibacterial Potential of Orange Peels Extracts Against Some Bacterial Isolates Associated With Tomato Rot in Jos Metropolis

Pandukur, S.G^*, Onyimba, LA,, Itelima, J.U, Plangnan, G.A, Dum, V.

Study was carried out to determine the antibacterial potential of orange peels extracts against some isolated bacteria associated with tomato rot in Jos. Rotten ripe tomato fruits were aseptically collected from Terminus and Farin-gada markets of Jos metropolis. The plant extract was obtained from dried orange peels by Soxhlet extraction using n-hexane. Agar well diffusion method was used for t...

Dec, 2017 pp. 3953-3961

7

Research Article

Microbiological Quality of Plantain (Musa paradisiacal)

Ajayi A.O.^*

This study on microorganisms associated with plantain sources was carried out in the Microbiological laboratory of Adekunle Ajasin University, Akungba-Akoko, Nigeria. Plantain {Musa Paradisiacal) is a rhizomatous nutritional perennial crop used as a source of starchy staple for millions of people in Nigeria. The total heterotrophic bacterial count for sample sources in cfu/mL ranged from 68 x 1...

Dec, 2017 pp. 3962-3969

8

Research Article

Beta-lactamase Genes in Multi-resistant Aeromonas spp. isolated from River and Aquaculture Water Sources in Nigeria

Chikwendu, C. L^*, Egbadon, E. O., Amadi, E.S, S. N. Ibe, Okpokwasili, G. C

This study examined multi-resistant Aeromonas spp. isolated from river and aquaculture water sources for determinants of resistant genes. These species can provide a reservoir for resistant genes capable of transfer to other water-borne and human pathogens. The isolates were confirmed with API 20NE. Resistance profiles of 206 Aeromonas isolates were determined for 11 antimicrobials by the Kirby...

Dec, 2017 pp. 3970-3978

9

Research Article

Antibacterial Activity of Lactic Acid Bacteria Isolated From Fermented Cereal Products Against Organisms Implicated in Gastrointestinal Tract Infections

Onipede, G.O^*, Fawole, A.O

The present study evaluates the in-vitro antibacterial activities of lactic acid bacteria (LAB) isolated from two fermented cereal products - ogi and kunun-zaki, against organisms implicated in gastrointestinal tract infections. A total of fifteen (15) LAB strains with 5 each from white maize agi, yellow maize ogi, and kunun-zaki were isolated and characterized. The LAB strains were tested agai...

Dec, 2017 pp. 3979-3985

10

Research Article

Studies of Antimicrobial Properties of Indigenous Medicinal Plants on Escherichia Coli 0157:H7 Isolates from Cattle Faeces

Umeh, S.I^*, Nwachukwu, I.N., Ozuruonye, CM.

The antimicrobial properties of indigenous medicinal plants Azadirachta indica (Neem seeds), Vernonia amygdalina Linn (Bitter leaves), Ocimum gratissimumlinn (Scent leaves), Moringa oleifera seeds, Zingiber offtcinale(G\\nger), Allium sativum (Garlic), Pterocapus santalinoides (Nturukpa) against Escherichia coli 0157:H7 isolated from cattle faeces using Cefixime-Pottasium Tellurite Sorbitol McC...

Dec, 2017 pp. 3986-3989

11

Research Article

Investigation of the Effects of Some Plant-Leaf Stem Preservatives on Palm Wine

Nwachukwu, I.N.^*

A preliminary investigation was carried out on the effect of certain plant leaves; Cola hispida, Mallotusoppositfoilus, Manihotesculenta and Newbouldia leavis on palm wine during storage. The leaves are used by traditional palm wine tappers and retailers in the south eastern Nigeria for the preservation of palm wine. Palm wine from Raphiaraphia and Elaensisguineensis were used in this study. Th...

Dec, 2017 pp. 3990-3995

12

Research Article

Comparative Study of Leaf Litter Decomposition of Two Exotic Tree Species In Afaka Kaduna State, Savanna Ecological Zone, Nigeria

Godwin Brian Onwumere^*, ThankGod Ositadinma Ndibe

There is high demand for nutrients by exotic tree species due to their rapid growth rate. The decomposition of leaf litters of two plant species, Tectona grandis and Eucalyptus camaldulensis were investigated using litterbag study. Soil and leaf litters were collected from six sampling sites in each plantation designated as El - E6 for E. camaldulensis and Tl - T6 for T. grandis respectively. Th...

Dec, 2017 pp. 3996-4009

13

Research Article

Antifungal Activities and Phytochemical Constituents of Nicotiana tabacum Leaf Extracts on Selected Dermatophytes.

Nwachukwu, I.N.^*

Extracts of Nicotiana tabacum were investigated for phytochemical constituents and antifungal activities against certain dermatophytes: Epidermophyton floccosum , Microsporum canis and Trichophyton rubrum . The alcohol extract was made using 95% ethanol and 95% methanol as extracting solvent in the ratio of 2:1. The hot water extract was made by boiling sample in distilled water. The phytochemi...

Dec, 2017 pp. 4010-4013

14

Research Article

Comparative Studies of Ugba Qualities Using Six(6) Processing Methods

Archibong, E. J.^*, Alor, C. J., Okoro, N. C

Ugba an indigenous Nigerian fermented food condiment is rich in substantial amount of protein, dietary fibre and minerals. Traditional processing method reduces the level of nutrients and minerals in the processed Ugba after long boiling. This study was therefore undertaken to determine the effects of processing methods on nutritional composition of Ugba. The control sample (CI) and the modifie...

Dec, 2017 pp. 4014-4021

15

Research Article

Keratinophilic Fungi Isolated from Second Hand Wears in Anambra State, Nigeria

Ekwealor Chito Clare^*, Eboh Chiamaka Cynthia, Uzondu Adanna Flora, EzembaChinyere Constance

Samples were collected from one hundred and twenty second hand wears and inoculated into Sabouraud Dextrose Agar Petri dishes supplemented with 0.5mglml cyclohexamide and 0.05mg/ml chloramphenicol. The fungal isolates were tested for their ability to degrade keratin using modified hair-baiting technique and eight species of fungi were recovered. The organisms isolated belong to dermatophyte and...

Dec, 2017 pp. 4022-4025

16

Research Article

Microbiological Assessment of different species of apples (Granny smith, Red delicious and Goldrennette) sold in Owerri Metropolis, Imo State

Ibe IJ^*, Emeh AA, Ononogbo CN, Nwaehiri UL, Anukam US, Nwakire EC

The microbiological assessment of different species of apples (Granny smith, Red delicious and Gold rennette apples) sold in Owerri Metropolis, Imo Slate was carried out using standard microbiological procedures. Three species of apples were bought from four different vendors at different locations within Owerri. Total viable bacterial counts recorded ranged from 1.0 x 103cfu/g to 1.32 105cfu/g...

Dec, 2017 pp. 4026-4031

17

Research Article

Prevalence of Urinary Tract Infections Among Pregnant Women Attending Antenatal Clinic In Some Selected Hospitals In Anambra State

Okonkwo Nonye G^*, Umeaku C. N.

Urinary Tract Infection is one of the most frequently seen medical complications in pregnancy. This study reports the prevalence of urinary tract infections among pregnant women attending antenatal clinics in some selected hospitals in Anambra State, Southeastern Nigeria. The aim of this work was to determine the prevalence of urinary tract infection among the pregnant women, the trimester peak...

Dec, 2017 pp. 4032-4039

18

Research Article

The Synergistic Effect of Garcinia Kola on Some Pathogenic Bacteria And Fungi

Bolarinwa O.O.^*, Adesokan LA, Onifade D.A., Fawole, A.O, Fawole O.

Due to increasing rate of antimicrobial drug resistance in recent years, this brought about research on use of natural plant for herbal medicine, by the use of Garcinia kola pericarp and the seed extract. The ethanolic extract shows a significant inhibition of growth against the tested bacteria (Klebsiella pneumoniall.OOmm,Escherichia coli 13.00mm, Staphylococcus aureus 10.00mm and Pseudomonas ...

Dec, 2017 pp. 4040-4046

19

Research Article

Qualitative and Quantitative Phytochemical Screening, and Antibacterial Activity of Phyllanthusamarus Plant Extracts

Adomi, Patience O^*

Phytochemical screening and antimicrobial activities of water, ethanol and methanol extracts of parts of Phyllanthusamarus plant were investigated. Qualitative results from the different extracts, showed the presence of alkaloids, tannins phenols, saponins and flavonoids. However, phenol was absent in ethanol seed extract, but present in other crude extracts obtained with water and ethanol. The...

Dec, 2017 pp. 4047-4053

20

Research Article

Screening of Some Antifungal Drugs on Fungal Isolates from Barbering Equipment

Chidi-Onuorah, Lilian C^*, Oyeka, Christie A, Kyrian-Ogbonna, Evelyn A., Ezeadila, Joachim O.

This work was aimed at ascertaining the antifungal potency of some antifungal drugs on fungal isolates from barbering equipment. Two hundred (200) samples were collected from barbering equipment pre and post sterilization. Samples were inoculated onto Sabouraud Dextrose Agar and incubated at room temperature for 5 7days.Subcultures were carried out to purify the isolates. One hundred (100) isol...

Dec, 2017 pp. 4054-4058

21

Research Article

Nasal Carriage of Cloxacillin-Resistant Staphylococcus aureus among University Students in Umudike, Abia State, Nigeria

Ngoka, D. I^*, Ekundayo, E. O

Strains of Staphylococcus aureus resistant to beta-lactamase stable antibiotics such as methicillin, oxacillin and cloxacillin, commonly called Methicillin resistant Staphylococcus aureus (MRSA) have become a serious public health concern worldwide. Nasal carriage is a major risk factor for infection with MRSA. In this study, nasal carriage rate of Cloxacillin resistant S. aureus was assessed a...

Dec, 2017 pp. 4059-4062

22

Research Article

Low Incidence of Extended Spectrum Beta Lactamase (ESBL) Detection From Enterobacteriaceae Isolated From Pig Faeces

Itaman, V.O^*, Nwankwo, E.O, Erhunmwunse, A.S, Adeleye, LA

This study assessed the low incidence of ESBL detect and associated antimicrobial/antibiotic resistance from Enterobacteriaceae isolated from pigs. A total of twenty-six (26) isolates from the forty-five (45) faecal piggery samples studied were Gram negative bacteria which were further characterized using standard biochemical procedures as : Citrobacter freundii 7(26.9%), E. coli 5(19.2%), Kleb...

Dec, 2017 pp. 4063-4067

23

Research Article

Effect of Nitrogen Sources on Crude Oil Utilization by Mangrove Bacterial Strains

Nwanyanwu, C. E^*, Adieze, Ifechukwu E., Nwauzor, A.R.

Batch experiment was conducted to investigate the effect of nitrogen source on biodegradation of crude oil by hydrocarbon utilizing bacteria isolated from Eagle Island mangrove ecosystem of Niger Delta, Nigeria. The organisms were identified as Pseudomonas sp. MW3 and Bacillus sp. SD4. The ability of the organisms to solubilize crude oil was indicated by having emulsification index values of 62...

Dec, 2017 pp. 4068-4073

24

Research Article

Profiles of aflatoxin biosynthetic key genes in non aflatoxigenic Aspergillus flavus isolated from in-process wheat flour from parts of Northern Nigeria

Wartu, J.R.^*, Whong, C.M.Z, Abdullahi, I.O, Ameh, J.B

Aflatoxigenic molds are strains that produce aflatoxins. This study used yeast extract sucrose agar supplemented with 0.3% beta methyl cyclodextrin and 0.6% sodium desoxycholate to differentiate between aflatoxigenic and atoxigenic strains. ELISA and multiplex PCR techniques were also employed to assess aflatoxin production and detect key aflatoxin genes (aflR-1, omt-A, ver-1, nor-1). Isolates 1,...

Dec, 2017 pp. 4072-4079