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Two cases of allergic alveolitis are described which resulted from traditional way of treating malaria using local herbs. The contents of the mixture used in this treatment are not universally known and there are local variations. A few of the items appear to be known in most areas qf the country. The particular antigen responsible for this reaction will only be found if the ingredients used in th...
"abstract": "A total of 120 samples from patients in two hospitals in Calabar were examined for Pseudomonas aeruginosa. About 45% of the cases — burns, ulcers, septic wounds—contained the organism. Sinks and wash basins from wards and theatres were found to constitute permanent reservoirs of the organism and a constant source of cross-infection. The relationship between factors such as age, du...
Quantitative bacteriology of 200 sputa from patients with chronic bronchopulmonary disorders was carried out. Mycobacterium tuberculosis was the most frequently isolated pathogen (19%), followed by Streptococcus pneumoniae (13.6%), Haemophilus influennzae (9.0%), Staphylococcus aureus (7%), Klebsiella pneumonia (4%), Pseudomonas aeruginosa (4%), Escherichia coli (4%), Nocardia asteroides in associ...
The bacterial flora of the vagina was assessed in healthy Nigerian women (twenty pregnant and twenty-four non-pregnant women). They all harboured many types of microorganisms in their vagina. The mean number of microbial types isolated was five for the pregnant and three for the non-pregnant women. Staphylococcus was detected in 18 of the pregnant women and 16 of the non-pregnant women. Amongst th...
West African Dwarf sheep were inoculated with a low mouse brain passage of Dugbe (Nairobi sheep disease group virus). Mild febrile reaction and low level of neutralising antibody were demonstrated in experimental animals
The range of produceable extracellular enzymes on solid media by the yam rot-causing pathogens was investigated, and the pathogens were found to produce abundant cellulase, polygalacturonase, amylase, and urease. All pathogens, except Botryodiplodia theobromae, also produced abundant lipase. Although all the pathogens produced phosphatase and pectate lyase, the production was low in Botryodiplodia...
Penicillium sclerotigenum Yamamoto grew in synthetic liquid medium with pectic substance as sole carbon source, releasing proteins which exhibited polygalacturonase (PG; poly (1,4-α-D-galacturonide) glycohydrolase, EC 32.1.15) and pectin lyase (PL; poly (methoxygalacturonide) lyase, EC 4.2.2.10) activities. The enzymes were also present in mycelial extracts and Penicillium-infected yam tissues but...
A study of the bacteria found growing on and spoiling beef in a meat shop in Ibadan is made. Those in the genera Micrococcus, Lactobacillus, Flavobacterium, and Pseudomonas were found to be the main spoilage organisms. Pseudomonas spp. were isolated under both warm and cold storage conditions and hence they constitute the major bacteria that will spoil stored meat.
The ability of Pseudomonas fluorescens to use carbon and nitrogen sources for growth was investigated by growing the organism in various carbon sources (Histidine, glucose, succinate, and glutamate) and nitrogen sources (Histidine, and ammonium sulphate) at pH 7.2 and 30°C temperature. The fastest growth rate was observed when glutamate served as the carbon source followed by glucose, while growth...
Perhaps the greatest danger associated with drinking water is contamination by human excrement (Bacteriological Examination of Water Supplies (BEWS), 1970). If such excrement contains pathogenic microorganisms, then consumers of the water may become infected by the diseases caused by the pathogens. The coliforms are generally present in large numbers in human excrement and can be detected in numbe...
A new synthetic medium was developed for growth of rhizobia. The medium consisted of mineral salts amended with 0.5% mannitol and one or two growth factors. The vitamins calcium pantothenate, thiamine mononitrate, inositol, and biotin stimulated rhizobial growth. In particular, calcium pantothenate was found to be almost as effective as a combination of all the other vitamins in promoting growth o...
Inoculation of 2 × 10⁶ cells of the yeasts Candida tropicalis and Pichia membranaefaciens into fruits of the large red pepper induced soft watery rot within 4 days of incubation at room temperature, 28 ± 2°C. Germination of the seeds on wet sterile filter papers was reduced to less than 10% in one week. Abnormal germination was observed after the 4th day, and dead seeds were prominent after the 6t...
A cold-labile trypsin-stimulated adenosine triphosphatase, ATPase (EC. 3.6.1.3) activity has been found associated with the partially purified cytoplasmic soluble fraction of Agrobacterium tumefaciens strain C-58. Chromatographic, electrophoretic, and catalytic studies are also reported. Trypsin activated the enzyme 31% at the pH optimum of between 8.0 and 9.0. The optimum temperature of activity ...
The ability of some soil bacteria to degrade the herbicides, chloroxuron (N-4-(4-chlorophenoxy) phenyl)-N,N-dimethyl-urea) and metobromuron (N-bromo-phenyl)-N-methoxy-N-methylurea) in laboratory experiments was investigated. The Rf and maximum absorption range values of the original herbicides and the end products after incubation with soil microorganisms indicated that new products were formed. T...
