Volume 40 (2026)

1

Research Article DOI: 10.67614/njm.2026.qde54ql4

Detection of Antimicrobial Resistance Genes in Alcaligenes faecalis Isolated from Urine Samples of HIV-Positive Individuals

Oladejo B. O.^*

Urinary tract infections (UTIs) pose a significant health challenge for individuals living with HIV, often involving opportunistic pathogens with escalating antimicrobial resistance. This study focused on Alcaligenes faecalis isolates recovered from urine samples of HIV-positive individuals attending a selected health facility in Akure, Nigeria. The study employed 16S rRNA gene sequencing for iden...

Jun, 2026 pp. 7840 - 7845

2

Research Article DOI: 10.67614/njm.2026.396g1pua

Isolation and Screening of Aspergillus niger Mutant for Improved Gluconic Acid Production

Ayodele E. O., Afolabi F. T.^*

Gluconic acid (GA, C6H12O7, or 2, 3, 4, 5, 6-pentahydroxyhexanoic acid) is a mild organic acid resulting from the oxidation of D-glucose. Gluconic acid has low toxicity, low corrosion and form complexes with metal ions giving it wide application in several industries. This study aimed to isolate and screen for Aspergillus niger mutant for improved gluconic acid production. A total of 204 A. niger ...

Jun, 2026 pp. 7943 - 7954

3

Research Article DOI: 10.67614/njm.2026.028ne9ai

Effect of Physiochemical Characteristics and Microbial Water Quality on Tensile Strength of Eichhornia crassipes Harvested from Four Rivers in Southwest Nigeria

Akinmola E. A.^*, Olalemi A. O., Emidun O. B., Adiji B. E.

This study investigated the influence of microbial water quality and physicochemical characteristics of the aquatic environment supporting the growth of water hyacinth on the mechanical properties of its fibre. Water hyacinth and water samples were collected from four rivers (Igbokoda, Makun, Epe and Badagry) in Southwest Nigeria. The load of faecal indicator bacteria in the water samples and the ...

Jun, 2026 pp. 7933 - 7942

4

Research Article DOI: 10.67614/njm.2026.qt3dn6ni

High Prevalence of Multidrug-Resistant Pseudomonas aeruginosa in Pre-Treated Pharmaceutical Wastewater: Evidence of Treatment Failure in Nigerian Manufacturing Facilities

Olaosebikan O. O., Nomeh O. L., Ogba R. C., Peter I. U.^*, Iroha I. R.

Pharmaceutical wastewater treatment plants are intended to mitigate environmental contamination, yet their efficacy against critical priority pathogens like Pseudomonas aeruginosa in low- resource settings remains unverified. This study isolated P. aeruginosa exclusively from pre-treated effluents across three Nigerian pharmaceutical facilities to assess treatment performance and compare inter-fac...

Jun, 2026 pp. 7921 - 7932

5

Research Article DOI: 10.67614/njm.2026.s0ja544x

Evaluation of Chicken Feathers Degraded by Keratinase for Poultry Feed Supplement

Okediji E. F.^*, Adegunloye D. V.

Chicken feathers pose a significant environmental challenge due to their high resistance to natural degradation. It accumulates in the environment and constitutes serious problems to human health. The study was conducted to degrade chicken feathers by keratinase from selected bacteria isolated from soil samples containing chicken feather waste and the resulting hydrolysate used as a supplement in ...

Jun, 2026 pp. 7913 - 7920

6

Research Article DOI: 10.67614/njm.2026.tt1kekj2

Occurrence of Multidrug-Resistant Escherichia coli in Abuja Wastewater Treatment Plant and Wupa River

Stella N Madueke, Ibangha I-A. I., Ozochi A. C., Onyeiwu S. C., Anyanwu C. U., Chigor V. N.^*

The discharge of multidrug-resistant bacteria into the environment can promote the transfer of resistance genes to clinically important bacteria, thereby posing a substantial public health risk. This study evaluated Escherichia coli isolated from the Abuja wastewater treatment plant and the Wupa River in Abuja, Nigeria, for multidrug resistance (MDR) and antibiotic resistance genes (ARGs). Isolate...

Jun, 2026 pp. 7900 - 7912

7

Research Article DOI: 10.67614/njm.2026.vccxp7qp

Biodegradation of Hydrocarbons by Bacillus cereus and Enterococcus casseliflavus Isolated from Crude oil Contaminated Seafood

Akomah-Abadaike O. N.^*, Mbolo P. F.

This study aims to determine the biodegradability capacity of Enterococcus casseliflavus and Bacillus isolated from crude oil contaminated seafood. Fresh seafood samples such as crude oil contaminated periwinkle and prawn were obtained. BOD, COD, pH, nitrate, phosphate, heavy metals (Cr, Cd, Pb, Zn), polyaromatic hydrocarbons (PAHs), total petroleum hydrocarbon (TPH} were determined. Standard micr...

Jun, 2026 pp. 7887 - 7899

8

Research Article DOI: 10.67614/njm.2026.8l64d5uy

Optimisation of Lipases from Fungi Associated with Palm Oil Mill Soils

Abiola O. F. A.^*

Lipases are industrially significant enzymes applied in food processing, detergents, pharmaceuticals, cosmetics, and biodiesel manufacture. Palm oil mill effluent (POME), though nutrient- rich and supportive of microbial growth, is also a major pollutant of the environment. Exploring lipolytic fungi from such soils provides a dual benefit of waste management and enzyme generation. Soil samples fro...

Jun, 2026 pp. 7880 - 7886

9

Research Article DOI: 10.67614/njm.2026.3f8blobr

Cephalosporin, Colistin, and Beta-Lactamase Inhibitor Resistance in Clinical Pseudomonas aeruginosa Isolates: Associations with Patient Age and Gender in a Nigerian Tertiary Hospital

Uzoeto H. O.^*, Mohammed I. D., Ukpai E. G., Peter I. U.

Pseudomonas aeruginosa is a leading cause of healthcare-associated infections with remarkable capacity for antimicrobial resistance. This study investigated the resistance profiles of clinical P. aeruginosa isolates, focusing on cephalosporins, colistin, and beta-lactamase inhibitors, and examined associations with age group and gender. A total of 250 clinical samples (150 urine, 100 wound swabs) ...

Jun, 2026 pp. 7870 - 7879

10

Research Article DOI: 10.67614/njm.2026.vdvtilqx

Prevalence, Antibiotic Susceptibility Profile, and Multiple Antibiotic Resistance Index of Gram-Negative Bacteria Isolated from Skin and Nasal Samples of Goats Bred in Abakaliki, Ebonyi State, Nigeria

Ukpai E. G.^*, Okwuonu C. A.

Antimicrobial resistance (AMR) is a critical global public health threat, with food-producing animals recognized as important reservoirs of multidrug-resistant (MDR) bacteria. In Nigeria, indiscriminate antibiotic use and weak regulatory frameworks have exacerbated resistant pathogen spread. Goats (Capra hircus) are widely reared in Abakaliki, Ebonyi State, serving as major meat and income sources...

Jun, 2026 pp. 7857 - 7869

11

Research Article DOI: 10.67614/njm.2026.3a85uf7r

Antibacterial Activities of Tiger Nuts Extracts against Bacteria Isolated from Diarrheagenic Stools Obtained from Selected Hospitals in Akure, Nigeria

Olalemi A. O., Adetoye A. A.^*

Diarrheal diseases remain a global health burden, especially in developing countries where antibiotic resistance complicates treatment. This study investigated the antibacterial activities of n-hexane and ethanolic extracts of tiger nut (Cyperus esculentus) against bacterial isolates obtained from diarrheagenic stool samples collected from selected hospitals in Akure, Nigeria. Stool samples were c...

Jun, 2026 pp. 7846 - 7856

12

Research Article DOI: 10.67614/njm.2026.05ny4e4b

Molecular Characterization and Taxonomic Profiling of Bacteria Isolates from Naturally Fermented Banana Wine

Akindele S. T., Okoh O. A.^*, Obafebi T. O., Raufu T. T.

Wine is a product of fermentation of fruits by microbes. Banana is a general term embracing a number of species or hybrid in the genus Musa of the family Musaceae. It is a seasonal and highly perishable fruit, which can be available all year round. This study was designed to identify the organisms responsible for the fermentation of wine. One species of banana was gotten from Ushin market in Ijebu...

Jun, 2026 pp. 7748 - 7754

13

Research Article DOI: 10.67614/njm.2026.skqqk1px

Assessment of Gut Fungi and Haematological Parameters of Patients with Different Blood Glucose Levels in Federal Medical Centre, Yenagoa

Isaiah O. G., Etebu E., Etebu E.^*

This study examined gut fungi and haematological parameters in 20 patients with varying blood glucose levels separated into diabetic (≥7.0 mmol/L, n=9), pre-diabetic (5.6-6.9 mmol/L, n=8), and normal (3.9-5.5 mmol/L, n=3) groups. Fungal species and haematological parameters were analyzed using molecular and standard methods. Total fungal populations were comparable across all three groups; mean Lo...

Jun, 2026 pp. 7829 - 7839

14

Research Article DOI: 10.67614/njm.2026.ltb6zmy4

Kocuria: A Potential Problem in Patients with Complicated Urinary Tract Infections

Imanyikwa O. E. E., Ezeonu I. M., Agbo M. C.^*

Kocuria species are Gram-positive, catalase positive and coagulase-negative cocci that were not previously associated with human infections; but have recently emerged as human pathogens. These organisms have been implicated in different infections including urinary tract infections (UTIs), mostly in patients with underlying diseases. The aim of this study was to investigate the possible involvemen...

Jun, 2026 pp. 7820 - 7828

15

Research Article DOI: 10.67614/njm.2026.40n1cbnw

Evaluation of Multidimensional Antimicrobial Efficacy of Extracts of Lawsonia inermis (Henna) Against Multidrug-Resistant Selected Pathogens

Hassan I. A.^*, Yusuf A. O., Taylor J. I., Oladetuyi A. P.

This study investigates the evaluation of multidimensional antimicrobial efficacy of extracts of Lawsonia inermis against multidrug-resistant selected pathogens. Antimicrobial assays of the two extract of Lawsonia inermis (aqueous and ethanolic) demonstrated against bacteria (Staphylococcus aureus, Bacillus cereus, Escherichia coli, and Pseudomonas aeruginosa), fungi (Candida albicans, Aspergillus...

Jun, 2026 pp. 7812 - 7819

16

Research Article DOI: 10.67614/njm.2026.mq9r5i1o

A Review of Urogenital Tuberculosis in Nigeria: Epidemiology, Pathogenesis, Clinical Manifestation, Diagnosis, Treatment Strategies, Risk Factors, and Prevention Control Measures

Garba D. E.^*, Dibua U. M. E., Oka C. U., OZOADIBE Ogechukwu Yvonne, Ajah M. I., Attah F.

Urogenital tuberculosis (UGTB) is a significant public health concern in Nigeria, where tuberculosis (TB) is endemic. Despite its importance, UGTB remains a neglected and underdiagnosed condition, leading to delayed diagnosis, inadequate treatment, and poor health outcomes. This review aims to provide an overview of the epidemiology, pathogenesis, clinical manifestations, diagnosis, treatment stra...

Jun, 2026 pp. 7802 - 7811

17

Research Article DOI: 10.67614/njm.2026.3ei7bn6t

Emergence of Multidrug-Resistant Salmonella enterica in Lagos: Insights from MALDI–TOF MS and β-Lactamase Profiling

Fakorede C. O.^*, Amisu K. O., Akinyemi K. O.

Non-typhoidal Salmonella (NTS) is a major cause of foodborne illness and invasive infections, particularly in low-resource settings. This study aimed to identify and characterise Salmonella enterica isolates from human, animal, and environmental sources in Lagos, Nigeria, using Matrix-Assisted Laser Desorption Ionisation–Time of Flight Mass Spectrometry (MALDI–TOF MS), and to evaluate their antimi...

Jun, 2026 pp. 7792 - 7801

18

Research Article DOI: 10.67614/njm.2026.ntkefqgd

Phytochemical Screening and Determination of Antibacterial Activity of Garlic Extracts against Clinical Isolates of Staphylococcus aureus

Bala H. K.^*, Shuaibu S., Mohammed A., Bala R. K.

The growing threat of drug resistant bacteria, particularly Staphylococus aureus, has become a pressing concern for global public health. Garlic (Allium sativum L.) widely used for culinary and medicinal purposes, is known for its broad spectrum antimicrobial activity, primarily due to the presence of allicin as its key bioactive compound. This study aimed to evaluate the phytochemical constituent...

Jun, 2026 pp. 7781 - 7791

19

Research Article DOI: 10.67614/njm.2026.pfvktzc5

Characterization and Antibiotic Susceptibility Profile of Vibrio cholerae Isolated from Stool of Patients Attending Ahmadu Bello University Medical Centre, Samaru-Zaria, Kaduna State, Nigeria

Ameh E. R., Aguoha E., Idoko M. O.^*, Bishop H. G., Otori M. O., Ubandoma A.

Cholera remains a significant public health concern worldwide, particularly in developing countries like Nigeria. It has the potential to cause widespread fatalities, spread rapidly, and disrupt travel and trade. This study aimed to characterize Vibrio cholerae isolated from stool samples of patients attending Ahmadu Bello University Medical Centre, Samaru-Zaria, Nigeria and assess the antibiotic ...

Jun, 2026 pp. 7770 - 7780

20

Research Article DOI: 10.67614/njm.2026.33t3scef

Exploring the Biocontrol Potential of Endophytes against Phytophthora megakarya in Cocoa Cultivation

Ayanwole S. A.^*, Oladigbolu Y. O., Adeji A. O., Laoye F. T., Adeniyi D. O., Arotupin Daniel Juwon

Cocoa (Theobroma cacao L., Malvaceae) is a perennial tree crop, which over the years been negatively affected by black pod disease which is caused by Phytophthora megakarya. This is a major pathogen responsible for black pod disease in cocoa, significantly reducing the yield and threatening cocoa production. Endophytes are asymptomatically within plant tissues, with antagonistic properties. The po...

Jun, 2026 pp. 7763 - 7769

21

Research Article DOI: 10.67614/njm.2026.a7x20aji

Microbiological Properties of Smoke-Dried Clarias gariepinus and Ethmalosa fimbriata Sold in Abraka, Eku and Obiaruku Markets, Delta State, Nigeria

Akpotor D. A.^*, Oyowe E., Amalokwu J. O.

Smoke-drying remains a sure fish processing-cum-preservation method in Nigeria. However, concerns persist about products microbiological safety afterwards particularly when sold in open markets. This study assessed the microbiological quality of smoked-dried Clarias gariepinus (Catfish) and Ethmalosa fimbriata (Bonga) sold in Abraka, Eku, and Obiaruku markets in Delta State, Nigeria. The fish samp...

Jun, 2026 pp. 7755 - 7762